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Utilization of Chlorella Biomass Grown in Waste Peels-Based Substrate for Simultaneous Production of Biofuel and Value-Added Products Under Microalgal Biorefinery Approach

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Abstract

Two indigenous microalgae strains Chlorella sorokiniana KMBM_I and Chlorella sorokiniana KMBM_K were tested for their growth and adaptability in the peel wastes. Growth kinetic parameters of the strains were analyzed in varying culture conditions. This study highlights the potential of a bio-based refinery of novel microalgal isolates in producing lipid, bioethanol and pigments etc. The isolates demonstrated significant amount of lipid, protein, carbohydrate and pigments like chlorophyll-a, chlorophyll-b and carotenoids. The spent biomass after extraction of lipids were reused and recycled for sustainable biofuel synthesis. Experimental results indicated that a waste-based refinery could lead to efficient production of value-added products from microalgae utilizing the organic wastes, in turn contributing to the establishment of a ‘green society’. The highest biomass yield of 2.56 ± 0.09 g L−1 and lipid content of 26.34 ± 0.24% was observed when the microalgal strain ‘K’ was cultivated in the mixed peel extract of potato, banana and sweet lime. Also, 7.16 ± 0.43 g L−1 bioethanol was derived from its spent biomass after the lipid extraction.

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Acknowledgements

The authors would like to thank the Central Instrument Facility (CIF), IIT Guwahati for the FESEM and EDX analysis and the Guwahati Biotech Park for the CHN analysis.

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Authors and Affiliations

  1. School of Energy Science and Engineering, Indian Institute of Technology Guwahati, Guwahati, 781039, India

    Barasa Malakar, Debasish Das & Kaustubha Mohanty

  2. Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, 781039, India

    Debasish Das

  3. Department of Chemical Engineering, Indian Institute of Technology Guwahati, Guwahati, 781039, India

    Kaustubha Mohanty

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  1. Barasa Malakar
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by BM. The first draft of the manuscript was written by BM and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Kaustubha Mohanty.

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Malakar, B., Das, D. & Mohanty, K. Utilization of Chlorella Biomass Grown in Waste Peels-Based Substrate for Simultaneous Production of Biofuel and Value-Added Products Under Microalgal Biorefinery Approach. Waste Biomass Valor 14, 3589–3601 (2023). https://doi.org/10.1007/s12649-023-02058-y

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