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Alternative Nitrogen Source for Producing Crude Extracted IAA and Suitable Method for Enhancing the Germination of Jerusalem Artichoke

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Abstract

Auxin is a useful compound which has an effect on plant root and shoot elongation. Thus, the aim of this study was to determine a suitable medium formula to produce indole-3-acetic acid (IAA). Adding various nitrogen sources such as beef extract, chicken manure solution, soybean residue, yeast extract, soybean powder and casein, supplemented with and without inducible substances such as tryptophan, optimized incubation temperature. IAA content in crude extraction was detected by TLC chromatography. Moreover, both the optimum concentration of IAA crude and the most suitable method (spreading or dipping) for application on Jerusalem artichoke were investigated. Five isolates revealed positive results on IAA product. The alternative medium for IAA production in the formula that consisted of soybean residue showed significantly highest contents of substances like IAA product when the rhizobacteria was cultured at 25 and 30 °C. Sixty µg/ml of crude extracted IAA was the optimum concentration, promoting Jerusalem artichoke (Helianthus tuberosus L.), growth with 100% germination. In addition, the root and shoot lengths exhibited were 3.79 and 7.14 cm, respectively. Dipping seed method was the most effective approach for encouraging seedling development, with a percentage of germination of 86.67 and root and shoot lengths of 4.48 and 5.39 cm, respectively. Our results demonstrated that a simple approach and appropriate concentration of crude extracted IAA could boost Jerusalem artichoke seedling development.

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Acknowledgements

The author would like to gratefully acknowledge Department of Microbiology, Faculty of Science, Khon Kaen University. The authors acknowledge to Mr. Matthew Graham Savage for proofing this manuscript.

Funding

This work was supported by Office of National Higher Education Science Research and Innovation Policy Council under Program Management Unit - B (Project B05F630053).

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Authors and Affiliations

  1. Graduate School of Microbiology, Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand

    Natnicha Onrit

  2. Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand

    Sophon Boonlue, Wiyada Mongkolthanaruk & Nuntavun Riddech

  3. Peanut and Jerusalem Artichoke Improvement for Functional Food Research Group, Khon Kaen University, Khon Kaen, 40002, Thailand

    Sanun Jogloy

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Correspondence to Nuntavun Riddech.

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Onrit, N., Boonlue, S., Mongkolthanaruk, W. et al. Alternative Nitrogen Source for Producing Crude Extracted IAA and Suitable Method for Enhancing the Germination of Jerusalem Artichoke. Waste Biomass Valor 14, 1497–1508 (2023). https://doi.org/10.1007/s12649-022-01970-z

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