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Radiation-Assisted Hydrolysis of Lignocellulosic Biomass. Mechanistic Study

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Abstract

High-energy irradiation, by γ-rays or electron beam, is used as a one-step penetrating pretreatment without heating nor chemicals before the enzymatic hydrolysis to convert the apple tree and pine biomass into glucose. The specific properties of various types of enzymes, isolated or mixed, are exploited to let conclude on the diverse effects of the irradiation. A complete conversion of the cellulose into glucose by the cellulase is reached after one MGy irradiation dose and none without irradiation, showing that all the cellulose bonds with lignin and hemicellulose are radiolytically broken by the irradiation pretreatment. The difference between the hydrolysis without and with isolated β-glucosidase (BG) indicates that also 60% of the free cellulose is depolymerized by the irradiation into oligosaccharides and cellobiose, finally hydrolyzed into glucose by the enzyme. The other part of crystalline and amorphous cellulose is hydrolyzed by the other enzymes contained in the cellulase, the cellobiohydrolase (CBH) and the endo 1,4 β-glucanase (EG), respectively. The small difference in glucose formation between the effects of the cellulase and of the mixture of endo 1,4 β-glucanase with β-glucosidase corresponds to the crystalline cellulose not yet amorphized by the irradiation. The specific enzymes efficiencies are studied not only to optimize the production of the hydrosoluble glucose, but also to disentangle quantitatively the overall radiolytic pretreatment/hydrolysis mechanism.

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Acknowledgements

The authors are deeply grateful to Pr F. Moussa (Institut Universitaire de Technologie, Université Paris-Saclay), Pr X. Coqueret (Institut de Chimie Moléculaire, Université de Reims Champagne Ardenne), and Pr F. Jérôme (Institut de Chimie des Milieux et Matériaux, Université de Poitiers) for their fruitful discussions on enzymes reactions and to Res. Engr J.-P. Larbre (Institut de Chimie Physique, Université Paris-Saclay) for his help on ELYSE experiments.

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  1. Université Paris-Saclay, UMR8000 CNRS, Institut de Chimie Physique, Rue Michel Magat, 91405, Orsay, France

    S. Al Gharib, M. Mostafavi & J. Belloni

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Al Gharib, S., Mostafavi, M. & Belloni, J. Radiation-Assisted Hydrolysis of Lignocellulosic Biomass. Mechanistic Study. Waste Biomass Valor 14, 1113–1122 (2023). https://doi.org/10.1007/s12649-022-01933-4

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