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Towards Valeric Acid Production from Riboflavin-Assisted Waste Sludge: pH-Dependent Fermentation and Microbial Community

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Abstract

The fermentative valeric acid production is a carbon-neutral method for the disposal of waste sludge in municipal wastewater treatment plants. To overcome the bottlenecks of energy-consuming and slow hydrolysis and acidification during the fermentation process, riboflavin was supplemented to enhance the biological redox reaction. The effect of pH (3, 5, 7, 9, 11) on the production of total volatile fatty acids (VFAs) and valeric acid from waste sludge, and on the shift of microbial community was investigated. The results indicated that the release of organic compounds and nitrogen, the production of VFAs and valeric acid, and the associated microbial community were pH-dependent in riboflavin-assisted sludge fermentation systems. Higher pH (9, 11) and shorter retention time (12 days) benefited the valeric acid production, which achieved to 190.8 (mg COD / g VSS), accounting for 76.6% of the total VFAs. Meanwhile, the solubilization of organics and ammonia was up to 43.7% and 38.4%, respectively. Alkaline condition in riboflavin-assisted fermentation system reduced the taxonomy categories and enriched the bacteria phyla of Firmicutes and the associated genera species, which were responsible for the acidification process related to valeric acid production. The study provided scientific insights into the recovery of valuable organic matter from waste sludge to achieve carbon-neutral and sustainable society.

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Data Availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors would like to thank Ministry of Science and Technology of the People’s Republic of China, and Zhejiang Society for Environmental Sciences for providing funding support for this research.

Funding

This research was funded by the National Key R&D Program of China (2019YFE0124600), Zhejiang Provincial Ecological & Environmental Research Project and Application (2021HT0028) and Graduate Scientific Research Foundation of Hangzhou Dianzi University (CXJJ2021032).

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Authors and Affiliations

  1. College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou, 310018, People’s Republic of China

    Binfang Shi, Jingang Huang, Wei Han, Shanshan Qiu, Dong Zhang & Junhong Tang

  2. The Belt and Road Information Research Institute, Hangzhou Dianzi University, Room 517, The 6th Building, Hangzhou, 310018, People’s Republic of China

    Jingang Huang, Wei Han, Shanshan Qiu & Pingzhi Hou

  3. Zhejiang Province Environmental Engineering Co. Ltd, Hangzhou, 310012, People’s Republic of China

    Yuanyuan Lin

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  1. Binfang Shi
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BS Investigation, validation, data curation, writing - original draft. JH Investigation, writing- original draft, review & editing, conceptualization, methodology, supervision, funding acquisition. YL Adising, funding acquisition. WH Writing- reviewing and editing. SQ Writing- reviewing and editing. DZ Investigation, writing- reviewing and editing. JT Adising, supervision. PH Funding acquisition, supervision, project administration.

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Shi, B., Huang, J., Lin, Y. et al. Towards Valeric Acid Production from Riboflavin-Assisted Waste Sludge: pH-Dependent Fermentation and Microbial Community. Waste Biomass Valor 14, 833–845 (2023). https://doi.org/10.1007/s12649-022-01900-z

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