Abstract
During surimi processing, large amount of the head & viscera is generated as by-product, which is either discarded or used for production of low value products such as fish feed. This study presents technology for sustainable utilization of by-products as high value-added products which indirectly reduces environmental pollution. Response Surface Methodology was used to define the optimal conditions for protein hydrolysates production. Different temperature (25–70 °C), time (20–120 min), and enzyme concentrations (0.05–0.2%) were optimized to obtain the maximum yield of Pink Perch Head & Viscera Protein Hydrolysate (PHVPH). The analysis of PHVPH revealed high amount of essential amino acids (35%) with 15% degree of hydrolysis, good functional properties, and moderate antioxidant properties (24.8%). The PHVPH was further microencapsulated using combination of wall material (maltodextrin, sodium alginate, gum Arabic and carboxyl methyl cellulose) to reduce the bitterness, fishy odor and hygroscopicity of PHVPH. Efficiency of microencapsulation process of PHVPH was assess by physiochemical properties, antioxidant activity, chemical bond (FTIR), microstructure (SEM) and sensory acceptability. The presence of PHVPH in the structure of microcapsule was proved by FTIR spectrometry. In addition, sensory evaluation of PHVPH and microencapsulated protein hydrolysate suggested that the microencapsulation process has been effective method in reducing the bitterness and odor of PHVPH powder and enhance its value in food formulation.
Graphical Abstract
Similar content being viewed by others
Data Availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
References
Naqash, S.Y., Nazeer, R.A.: Antioxidant and functional properties of protein hydrolysates from Pink Perch (Nemipterus japonicus) muscle. J. Food Sci. Technol. 50(5), 972–978 (2013). https://doi.org/10.1007/s13197-011-0416-y
Gajanan, P.G., Elavarasan, K.: Bioactive and functional properties of protein hydrolysates from fish frame processing waste using plant proteases. Environ. Sci. Pollut. Res. 23(24), 24901–24911 (2016). https://doi.org/10.1007/s11356-016-7618-9
Wu, S.: Effect of pullulan on gel properties of Scomberomorus niphonius surimi. Int. J. Biol. Macromol. 93, 1118–1120 (2016). https://doi.org/10.1016/j.ijbiomac.2016.09.085
Sun, L., Sun, J., Thavaraj, P., Yang, X., Guo, Y.: Effects of thinned young apple polyphenols on the quality of grass carp (Ctenopharyngodon idellus) surimi during cold storage. Food Chem. 224, 372–381 (2017). https://doi.org/10.1016/j.foodchem.2016.12.097
Sultan, F.A., Routroy, S., Thakur, M.: Introducing traceability in the Indian Surimi supply chain. Mater. Today 28, 964–969 (2020). https://doi.org/10.1016/j.matpr.2019.12.333
Jafarpour, A., Gomes, R.M., Gregersen, S., Sloth, J.J., Jacobsen, C., Sørensen, A.D.M.: Characterization of cod (Gadus morhua) frame composition and its valorization by enzymatic hydrolysis. J. Food Compos. Anal. 89, 103469 (2020). https://doi.org/10.1016/j.jfca.2020.103469
Kim, N.: A comparative study on properties of fish meat hydrolysates produced by an enzymatic process at high pressure. Food Sci. Biotechnol. 29(1), 75–83 (2020). https://doi.org/10.1007/s10068-019-00648-y
Siewe, F.B., Kudre, T.G., Narayan, B.: Optimization of ultrasound-assisted enzymatic extraction conditions of umami compounds from fish by-products using the combination of fractional factorial design and central composite design. Food Chem. 334, 127498 (2021). https://doi.org/10.1016/j.foodchem.2020.127498
Rustad, T., Storrø, I., Slizyte, R.: Possibilities for the utilisation of marine by-products. Int. J. Food Sci. Technol. (2011). https://doi.org/10.1111/j.1365-2621.2011.02736.x
Halim, N.R.A., Yusof, H.M., Sarbon, N.M.: Functional and bioactive properties of fish protein hydolysates and peptides: a comprehensive review. Trends Food Sci. Technol. 51, 24–33 (2016). https://doi.org/10.1016/j.tifs.2016.02.007
Vieira, E.F., Pinho, O., Ferreira, I.M.: Bio-functional properties of sardine protein hydrolysates obtained by brewer’s spent yeast and commercial proteases. J. Sci. Food Agric. 97(15), 5414–5422 (2017). https://doi.org/10.1002/jsfa.8432
Corrêa-Filho, L.C., Moldão-Martins, M., Alves, V.D.: Advances in the application of microcapsules as carriers of functional compounds for food products. Appl. Sci. 9(3), 571 (2019). https://doi.org/10.3390/app9030571
Jeyakumari, A., Zynudheen, A.A., Parvathy, U.: Microencapsulation of bioactive food ingredients and controlled release—a review. MOJ Food Process. Technol. 2(6), 59 (2016). https://doi.org/10.15406/mojfpt.2016.02.00059
Horwitz, W.: Official Method of Analysis, 18th edn. Association of Officiating Analytical Chemists International Maryland, Rockville (2005)
Steinsholm, S., Oterhals, Å., Underhaug, J., Aspevik, T.: Emulsion and surface-active properties of fish soluble based on direct extraction and after hydrolysis of Atlantic cod and Atlantic salmon backbones. Foods 10(1), 38 (2021). https://doi.org/10.3390/foods10010038
Taylor, W.H.: Formol titration: an evaluation of its various modifications. Analyst 82(976), 488–498 (1957). https://doi.org/10.1039/AN9578200488
Slizyte, R., Rommi, K., Mozuraityte, R., Eck, P., Five, K., Rustad, T.: Bioactivities of fish protein hydrolysates from defatted salmon backbones. Biotechnol. Rep. 11, 99–109 (2016). https://doi.org/10.1016/j.btre.2016.08.003
Alsmeyer, R.H., Cunningham, A.E., Happich, M.L.: Equations predicting PER from amino acid analysis. Food Technol. 28, 34–40 (1974)
Lee, Y.B., Elliott, J.G., Rickansrud, D.A., Hagberg, E.Y.C.: Predicting protein efficiency ratio by the chemical determination of connective tissue content in meat. J. Food Sci. 43, 1359–1362 (1978). https://doi.org/10.1111/j.1365-2621.1978.tb02490.x
Opheim, M., Šližytė, R., Sterten, H., Provan, F., Larssen, E., Kjos, N.P.: Hydrolysis of Atlantic salmon (Salmo salar) rest raw materials—effect of raw material and processing on composition, nutritional value, and potential bioactive peptides in the hydrolysates. Process Biochem. 50(8), 1247–1257 (2015). https://doi.org/10.1016/j.procbio.2015.04.017
Sila, A., Sayari, N., Balti, R., Martinez-Alvarez, O., Nedjar-Arroume, N., Moncef, N., Bougatef, A.: Biochemical and antioxidant properties of peptidic fraction of carotenoproteins generated from shrimp by-products by enzymatic hydrolysis. Food Chem. 148, 445–452 (2014). https://doi.org/10.1016/j.foodchem.2013.05.146
Salem, R.B.S.B., Bkhairia, I., Abdelhedi, O., Nasri, M.: Octopus vulgaris protein hydrolysates: characterization, antioxidant and functional properties. J. Food Sci. Technol. 54(6), 1442–1454 (2017). https://doi.org/10.1007/s13197-017-2567-y
Egerton, S., Culloty, S., Whooley, J., Stanton, C., Ross, R.P.: Characterization of protein hydrolysates from blue whiting (Micromesistius poutassou) and their application in beverage fortification. Food Chem. 245, 698–706 (2018). https://doi.org/10.1016/j.foodchem.2017.10.107
Schmedes, A., Hølmer, G.: A new thiobarbituric acid (TBA) method for determining free malondialdehyde (MDA) and hydroperoxides selectively as a measure of lipid peroxidation. J. Am. Oil Chem. Soc. 66(6), 813–817 (1989). https://doi.org/10.1007/BF02653674
Maqsoudlou, A., Mahoonak, A.S., Mohebodini, H., Koushki, V.: Stability and structural properties of bee pollen protein hydrolysate microencapsulated using maltodextrin and whey protein concentrate. Heliyon 6(5), e03731 (2020). https://doi.org/10.1016/j.heliyon.2020.e03731
Mendanha, D.V., Ortiz, S.E.M., Favaro-Trindade, C.S., Mauri, A., Monterrey-Quintero, E.S., Thomazini, M.: Microencapsulation of casein hydrolysate by complex coacervation with SPI/pectin. Food Res. Int. 42(8), 1099–1104 (2009). https://doi.org/10.1016/j.foodres.2009.05.007
Lu, T.S., Yiao, S.Y., Lim, K., Jensen, R.V., Hsiao, L.L.: Interpretation of biological and mechanical variations between the Lowry versus Bradford method for protein quantification. N. Am. J. Med. Sci. 2(7), 325 (2010). https://doi.org/10.4297/najms.2010.2325
Cai, Y.Z., Corke, H.: Production and properties of spray-dried Amaranthus betacyanin pigments. J. Food Sci. (N. Y.) 65, 1248–1252 (2000). https://doi.org/10.1111/j.1365-2621.2000.tb10273.x
Cano-Chauca, M., Stringheta, P.C., Ramos, A.M., Cal-Vidal, J.: Effect of the carriers on the microstructure of mango powder obtained by spray-drying and its functional characterization. Innov. Food Sci. Emerg. Technol. 6, 420–428 (2005). https://doi.org/10.1016/j.ifset.2005.05.003
Hashemi, A., Jafarpour, A.: Rheological and microstructural properties of beef sausage batter formulated with fish fillet mince. J. Food Sci. Technol. 53(1), 601–610 (2016). https://doi.org/10.1007/s13197-015-2052-4
Jangam, S.V., Thorat, B.N.: Optimization of spray drying of ginger extract. Dry. Technol. 28(12), 1426–1434 (2010). https://doi.org/10.1080/07373937.2010.482699
Thiansilakul, Y., Benjakul, S., Shahidi, F.: Antioxidative activity of protein hydrolysate from round scad muscle using Alcalase and flavourzyme. J. Food Biochem. 31(2), 266–287 (2007). https://doi.org/10.1111/j.1745-4514.2007.00111.x
Nenadis, N., Lazaridou, O., Tsimidou, M.Z.: Use of reference compounds in antioxidant activity assessment. J. Agric. Food Chem. 55(14), 5452–5460 (2007). https://doi.org/10.1021/jf070473q
Flores-Belmont, I.A., Palou, E., López-Malo, A., Jiménez-Munguía, M.T.: Simple and double microencapsulation of Lactobacillus acidophilus with chitosan using spray drying. Int. J. Food Stud. (2015). https://doi.org/10.7455/ijfs/4.2.2015.a7
Klomklao, S., Benjakul, S.: Utilization of tuna processing byproducts: Protein hydrolysate from skipjack tuna (Katsuwonus pelamis) viscera. J. Food Process. Preserv. 41(3), e12970 (2016). https://doi.org/10.1111/jfpp.12970
Rajabzadeh, M., Pourashouri, P., Shabanpour, B., Alishahi, A.: Amino acid composition, antioxidant and functional properties of protein hydrolysates from the roe of rainbow trout (Oncorhynchus mykiss). Int. J. Food Sci. Technol. 53(2), 313–319 (2017). https://doi.org/10.1111/ijfs.13587
Tacias-Pascacio, V.G., Morellon-Sterling, R., Siar, E.H., Tavano, O., Berenguer-Murcia, Á., Fernandez-Lafuente, R.: Use of Alcalase in the production of bioactive peptides: a review. Int. J. Biol. Macromol. 165, 2143–2196 (2020). https://doi.org/10.1016/j.ijbiomac.2020.10.060
Sovik, S.L., Rustad, T.: Effect of season and fishing ground on the activity of cathepsin B and collagenase in by-products from cod species. LWT Food Sci. Technol. 39(1), 43–53 (2006). https://doi.org/10.1016/j.lwt.2004.11.006
Nam, P.V., Van Hoa, N., Anh, T.T.L., Trung, T.S.: Towards zero-waste recovery of bioactive compounds from catfish (Pangasius hypophthalmus) by-products using an enzymatic method. Waste Biomass Valoriz. 11(8), 4195–4206 (2019). https://doi.org/10.1007/s12649-019-00758-y
Wisuthiphaet, N., Kongruang, S., Chamcheun, C.: Production of fish protein hydrolysates by acid and enzymatic hydrolysis. J. Med. Bioeng. (2015). https://doi.org/10.12720/jomb.4.6.466-470
Tacias-Pascacio, V.G., Castaneda-Valbuena, D., Morellon-Sterling, R., Tavano, O., Berenguer-Murcia, Á., Vela-Gutiérrez, G., Fernandez-Lafuente, R.: Bioactive peptides from fisheries residues: a review of use of papain in proteolysis reactions. Int. J. Biol. Macromol. 184, 415–428 (2021). https://doi.org/10.1016/j.ijbiomac.2021.06.076
Sila, A., Bougatef, A.: Antioxidant peptides from marine by-products: isolation, identification and application in food systems. A review. J. Funct. Foods 21, 10–26 (2016). https://doi.org/10.1016/j.jff.2015.11.007
Latorres, J.M., Rios, D.G., Saggiomo, G., Wasielesky, W., Prentice-Hernandez, C.: Functional and antioxidant properties of protein hydrolysates obtained from white shrimp (Litopenaeus vannamei). J. Food Sci. Technol. 55(2), 721–729 (2018). https://doi.org/10.1007/s13197-017-2983-z
Chalamaiah, M., Hemalatha, R., Jyothirmayi, T., Diwan, P.V., Bhaskarachary, K., Vajreswari, A., Dinesh Kumar, B.: Chemical composition and immunomodulatory effects of enzymatic protein hydrolysates from common carp (Cyprinus carpio) egg. Nutrition 31(2), 388–398 (2015). https://doi.org/10.1016/j.nut.2014.08.006
Bhilave, M.P., Bhosale, S., Nadaf, B.: Protein efficiency ratio (PER) of Ctenopharenge donidella fed on soybean formulated feed. Biol. Forum 4(1), 79–81 (2012)
Zainol, M.K., Tan, R.C., Mohd Zin, Z., Ahmad, A., Danish-Daniel, M.: Effectiveness of Toothpony (Gazza minuta) protein hydrolysate on reducing oil uptake upon deep-frying. Food Res. 4(3), 805–813 (2020). https://doi.org/10.26656/fr.2017.4(3).392
Mohamed, G., Sulieman, A., Soliman, N., Bassiuny, S.: Fortification of biscuits with fish protein concentrate. World J. Dairy Food Sci. 9(2), 242–249 (2014). https://doi.org/10.5829/idosi.wjdfs.2014.9.2.1142
Cheng, I.C., Liao, J.X., Ciou, J.Y., Huang, L.T., Chen, Y.W., Hou, C.Y.: Characterization of protein hydrolysates from eel (Anguilla marmorata) and their application in herbal eel extracts. Catalysts 10(2), 205 (2020). https://doi.org/10.3390/catal10020205
Chew, R.M., Ahmad, A., Mohtar, N.F., Rusli, N.D., Zainol, M.K.: Physicochemical and sensory properties of deep fried battered squid containing Brownstripe red snapper (Lutjanus vitta) protein hydrolysate. Food Res. 4(4), 1245–1253 (2020). https://doi.org/10.26656/fr.2017.4(4).083
Kempka, A.P., Prestes, R.C.: Foaming and emulsifying capacity, foam and emulsion stability of proteins of porcine blood: determination at different values of pH and concentrations. Rev. Bras. Tecnol. Agroind. 9(1), 1797–1809 (2015). https://doi.org/10.3895/rbta.v9n1.2065
Abdollahi, M., Undeland, I.: Structural, functional, and sensorial properties of protein isolate produced from salmon, cod, and herring by-products. Food Bioprocess Technol. 11(9), 1733–1749 (2018). https://doi.org/10.1007/s11947-018-2138-x
Priatni, S., Harimadi, K., Buana, E., Kosasih, W., Rohmatussolihat, R.: Production and characterization of spray-dried swamp eel (Monopterus albus) protein hydrolysate prepared by papain. Sains Malays. 49(3), 545–552 (2020). https://doi.org/10.17576/jsm-2020-4903-09
Hamzah, M., Shaik, M.I., Sarbon, N.M.: Effect of fish protein hydrolysate on physicochemical properties and oxidative stability of shortfin scad (Decapterus macrosoma) emulsion sausage. Food Res. 5(3), 225–235 (2021)
Yang, M., Liang, Z., Wang, L., Qi, M., Luo, Z., Li, L.: Microencapsulation delivery system in food industry—challenge and the way forward. Adv. Polym. Technol. (2020). https://doi.org/10.1155/2020/7531810
Comunian, T.A., da Silva Anthero, A.G., Bezerra, E.O., Moraes, I.C.F., Hubinger, M.D.: Encapsulation of pomegranate seed oil by emulsification followed by spray drying: evaluation of different biopolymers and their effect on particle properties. Food Bioprocess Technol. 13(1), 53–66 (2020). https://doi.org/10.1007/s11947-019-02380-1
Sarabandi, K., Mahoonak, A.S., Hamishekar, H., Ghorbani, M., Jafari, S.M.: Microencapsulation of casein hydrolysates: physicochemical, antioxidant and microstructure properties. J. Food Eng. 237, 86–95 (2018). https://doi.org/10.1016/j.jfoodeng.2018.05.036
Hassan, M.A., Deepitha, R.P., Xavier, K.M., Gupta, S., Nayak, B.B., Balange, A.K.: Evaluation of the properties of spray dried visceral protein hydrolysate from Pangasianodon hypophthalmus (Sauvage, 1978) extracted by enzymatic and chemical methods. Waste Biomass Valoriz. 10(9), 2547–2558 (2018). https://doi.org/10.1007/s12649-018-0302-1
Wang, B., Li, L., Chi, C.F., Ma, J.H., Luo, H.Y., Xu, Y.F.: Purification and characterization of a novel antioxidant peptide derived from blue mussel (Mytilus edulis) protein hydrolysate. Food Chem. 138(2–3), 1713–1719 (2013). https://doi.org/10.1016/j.foodchem.2012.12.002
Vázquez, J.A., Meduíña, A., Durán, A.I., Nogueira, M., Fernández-Compás, A., Pérez-Martín, R.I., Rodríguez-Amado, I.: Production of valuable compounds and bioactive metabolites from by-products of fish discards using chemical processing, enzymatic hydrolysis and bacterial fermentation. Mar. Drugs 17(3), 139 (2019). https://doi.org/10.3390/md17030139
Lima, K.O., de Quadros, C.D.C., da Rocha, M., de Lacerda, J.T.J.G., Juliano, M.A., Dias, M., Prentice, C.: Bioactivity and bioaccessibility of protein hydrolyzates from industrial byproducts of stripped weakfish (Cynoscion guatucupa). LWT 111, 408–413 (2019). https://doi.org/10.1016/j.lwt.2019.05.043
You, L., Zhao, M., Regenstein, J.M., Ren, J.: Changes in the antioxidant activity of loach (Misgurnus anguillicaudatus) protein hydrolysates during a simulated gastrointestinal digestion. Food Chem. 120, 810–816 (2010). https://doi.org/10.1016/j.foodchem.2009.11.018
Rosenberg, M., Rosenberg, Y., Frenkel, F.: Microencapsulation of model oil in wall matrices consisting of SPI and maltodextrins. AIMS Agric. Food 1(1), 33–51 (2016). https://doi.org/10.3934/agrfood.2016.1.33
Annamalai, J., Aliyamveetil Abubacker, Z., Lakshmi, N.M., Unnikrishnan, P.: Microencapsulation of fish oil using fish protein hydrolysate, maltodextrin, and gum Arabic: effect on structural and oxidative stability. J. Aquat. Food Prod. Technol. 29(3), 293–306 (2020). https://doi.org/10.1080/10498850.2020.1723765
Gómez-Mascaraque, L.G., Miralles, B., Recio, I., López-Rubio, A.: Microencapsulation of a whey protein hydrolysate within micro-hydrogels: impact on gastrointestinal stability and potential for functional yoghurt development. J. Funct. Foods 26, 290–300 (2016). https://doi.org/10.1016/j.jff.2016.08.006
Acknowledgements
This work was supported by the Department of Biotechnology, Government of India [Grant Number BT/IN/INNO-INDIGO/12/NK/2017-18]; The Research Council of Norway [Grant Number 281262].
Funding
This work was supported by the Department of Biotechnology [Grant Number BT/IN/INNO-INDIGO/12/NK/2017-18]; The Research Council of Norway [Grant Number 281262].
Author information
Authors and Affiliations
Contributions
Conceptualization: NK, RS; Methodology: NK, RS, AK; Formal analysis: NK, AK, RS; Investigation: NK, RS, AK, K; Writing—original draft preparation—review and editing: AK, NK, RS; Review Draft: NK, RS, AK, K; Funding acquisition: NK, RS.
Corresponding author
Ethics declarations
Conflict of interest
All authors states that there is no conflict of interest.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Kumari, A., Kaushik, N., Slizyte, R. et al. Production and Microencapsulation of Protein Hydrolysate of Pink Perch (Nemipterus japonicus) By-Products Obtained from Surimi Industry for Its Sustainable Utilization. Waste Biomass Valor 14, 209–226 (2023). https://doi.org/10.1007/s12649-022-01853-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12649-022-01853-3