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Effect of Exogenic Humic Substances on Various Growth Endpoints of Alternaria alternata and Trichoderma harzianum in the Experimental Conditions

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Abstract

Industrial humic products (HPs) manufactured from various organic matter resources, including fossils, peat, lake-bottom sediments and organic waste materials, have found multiple applications in soil environments. Fungi play a crucial role in the turnover of humic substances (HSs). However, the data on the biological effects of HSs on soil fungi are contradictory. Revealing mechanisms of how HSs interact with soil filamentous fungi is essential for understanding a sustainable soil system. Here we study the responses of phytopathogenic Alternaria alternata and the potential antagonist Trichoderma harzianum, to exogenic HPs (from leonardite and lignosulfonate) at two concentrations (0.1 and 0.02%) in growth media with varying sucrose concentrations (0, 3 and 30 g/L). We assessed the fungal growth endpoints including the mycelium biomass accumulation, fungal colony size and conidia production. Our results demonstrate the significant relationships between the extent of inhibitory/stimulation effect of HPs on the investigated fungi and the carbon status of the growth medium, some features of the fungal species, as well as the type and concentration of the introduced HPs. The responses of plant pathogens correlated specifically with various types of HPs. HP from lignosulfonate exhibited a more stimulating effect on A. alternata compared to the effect of HP from leonardite.

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Acknowledgements

The research was supported by the Russian Foundation of Basic Research No. 18-04-01218.

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Authors and Affiliations

  1. Department of Biology, Pirogov Russian National Research Medical University, Ostrovitianov Str. 1, Moscow, Russia, 117997

    Elena V. Fedoseeva

  2. Lomonosov Moscow State University, Leninskie Gory 1-12, Moscow, Russia, 119991

    Elena V. Fedoseeva, Svetlana V. Patsaeva, Daria A. Khundzhua & Vera A. Terekhova

  3. Skolkovo Institute of Science and Technology, 30 Bolshoy Bulvar, Moscow, Russia, 121205

    Maria A. Pukalchik

  4. Institute of Ecology and Evolution RAS, 33 Leninskij Prosp, Moscow, Russia, 119071

    Vera A. Terekhova

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  1. Elena V. Fedoseeva
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Correspondence to Elena V. Fedoseeva.

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Fedoseeva, E.V., Patsaeva, S.V., Khundzhua, D.A. et al. Effect of Exogenic Humic Substances on Various Growth Endpoints of Alternaria alternata and Trichoderma harzianum in the Experimental Conditions. Waste Biomass Valor 12, 211–222 (2021). https://doi.org/10.1007/s12649-020-00974-x

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