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Evaluation of the Effect of Different Extraction Techniques on Sour Cherry Pomace Phenolic Content and Antioxidant Activity and Determination of Phenolic Compounds by FTIR and HPLC

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Abstract

Sour cherry pomace derived from mashed cherries that maintains the color and beneficial properties of the cherries (Prunus cerasus L.). In this study, microwave-assisted extraction (MAE) (900 W for 30, 60 and 90 s.), high hydrostatic pressure (HHP) (400 and 500 MPa for 1, 5 and 10 min at 20 °C) and ultrasonic-assisted extraction (UAE) (for 5,10 and 15 min with a power of 100%) was used as novel processes. Total phenolic content (TPC), morphological changes, antioxidant activity (AA) and structural changes of cherry pomace were measured by the Folin–Ciocalteu assay, high performance liquid chromatography (HPLC), DPPH-scavenging activity, scanning electron microscopy (SEM) and Fourier transform infrared spectrometry (FTIR). According to the results, all novel technologies (MAE-HHP-UAE) increase PC and AA with respect to conventional solvent extraction method (50 °C and 30 min) (CSE). Among novel technologies, MAE (90 s) had highest TPC (275.31 ± 4.26 GAE/100 g FW) and also had highest antioxidant activity (89.9 ± 0.22%). The lowest TPC and AA values were 108.36 ± 3.99 mg gallic acid equivalent (GAE)/100 g fresh weight (FW) and 71.30 ± 1.21% in CSE, respectively. According to FTIR results, there are no significant structural changes in samples when different extraction techniques were applied. Individual phenolics were quantified by using HPLC for different extraction techniques.

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  1. Food Engineering Department, Engineering Faculty, Nigde Omer Halisdemir University, Nigde, Turkey

    İlhami Okur, Cem Baltacıoğlu & Hande Baltacıoğlu

  2. Food Engineering Department, Engineering Faculty, Middle East Technical University, Ankara, Turkey

    İlhami Okur & Hami Alpas

  3. Food Engineering Department, Agriculture Faculty, Cukurova University, Adana, Turkey

    Erdal Ağçam

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Okur, İ., Baltacıoğlu, C., Ağçam, E. et al. Evaluation of the Effect of Different Extraction Techniques on Sour Cherry Pomace Phenolic Content and Antioxidant Activity and Determination of Phenolic Compounds by FTIR and HPLC. Waste Biomass Valor 10, 3545–3555 (2019). https://doi.org/10.1007/s12649-019-00771-1

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