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Cellulase Production from Pre-treated Pea Hulls Using Trichoderma reesei Under Submerged Fermentation

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Abstract

Purpose

Pea hulls have a significant contribution to agricultural waste and are left unattended. It has a rich nutrient media and may have application in waste utilization. In this investigation, the potential of pea hulls for the production of cellulase has been explored.

Methods

The effect of H2O2 concentration, agitation speed and harvesting time on cellulase production by Trichoderma reesei QM9414 under submerged fermentation at constant pH of 5.0 and temperature 30 ± 0.1 °C were studied.

Results

The maximum filter paper (FP) cellulase activity of 0.372 ± 0.019 U/mL was obtained at 91 h incubation period and 120 rpm agitation speed. Based on the optimized results of fermentation parameters, 120 rpm agitation speed, 5% H2O2 concentration and 91 h harvesting time was recommended for efficient cellulase production. The effect of harvesting time on protein, reducing sugar and cellulase activity was pre-dominant. The purified cellulase enzyme specific activity was recorded as 13.8 U/mL.

Conclusions

The results indicate that the production of cellulase from green pea hulls may provide a novel and economical solution for industrial waste disposal.

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Abbreviations

β 0, β i, β ij, β ii :

Model coefficients

p:

Number of explanatory variables (excluding constants)

y i :

Observed data

\({\bar {y}_l}\) :

Mean of observed data

\({\hat {y}_l}\) :

Predicted data

N:

Sample size

X i,j :

Independent variables

Y :

Dependent variable

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Acknowledgements

This work was submitted as thesis in partial fulfillment for the requirement of the degree of Master of Technology of the first author. The authors thankfully acknowledge the financial assistance received under University funded project, G. B. Pant University of Agriculture & Technology, Pantnagar. We extend our gratitude to all the colleagues of the department for helping us in the manual separation of pea from pea pods.

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Authors and Affiliations

  1. Department of Post Harvest Process and Food Engineering, College of Technology, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, 263145, India

    Ranjna Sirohi, Anupama Singh, Ayon Tarafdar, Navin Chandra Shahi & Anurag Kushwaha

  2. Department of Biochemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, 263145, India

    Ashok Kumar Verma

Authors
  1. Ranjna Sirohi
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  2. Anupama Singh
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  3. Ayon Tarafdar
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  4. Navin Chandra Shahi
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  6. Anurag Kushwaha
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Correspondence to Ranjna Sirohi.

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Sirohi, R., Singh, A., Tarafdar, A. et al. Cellulase Production from Pre-treated Pea Hulls Using Trichoderma reesei Under Submerged Fermentation. Waste Biomass Valor 10, 2651–2659 (2019). https://doi.org/10.1007/s12649-018-0271-4

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