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Enzymatic Hydrolysis of Fruit Peels and Other Lignocellulosic Biomass as a Source of Sugar

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Abstract

Glucose and reducing sugars were obtained from corn cobs, orange and pomegranate peels by employing different enzymatic hydrolysis and pretreatment strategies. For the hydrolysis of corn cobs, effect of alkaline pretreatment was examined. Effect of cellobiase loading on hydrolysis was also investigated. Glucose content was measured using a Blood Glucose Monitor. When cellobiase was also added to hydrolysate, glucose and TRS yield increased from 20 and 34% respectively. Unlike corncobs, alkaline pretreatment was not applied to pomegranate and orange peels due to low lignin content. For the hydrolysis of these substrates, a pectic enzyme mixture was also used. Reasonable glucose and total reducing sugar yield from pomegranate and orange peel hydrolysis was achieved without any pretreatment. It was shown that while efficient corn cob hydrolysis needed synergistic effect of cellulase and β-glucosidase combination, fruit peels hydrolysis needed synergistic effect of cellulase, β-glucosidase and pectinase.

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Acknowledgements

The authors gratefully acknowledge the financial support of the National and Scientific Technological Council of Turkey, TEYDEB Program (1509) with proposal number 9120050. The project was partly sponsored by EU under 7th framework programme (ERA-IB) headed by Biopolis Biotech Company in Valencia, Spain. Authors also would like to thank Prof. Dr. Ufuk Bolukbasi for her suggestions and contributions during the hydrolysis experiments.

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Authors and Affiliations

  1. Department of Food Engineering, Middle East Technical University, Ankara, Turkey

    Pelin Pocan, Mecit Halil Oztop & Haluk Hamamci

  2. H2Biyotek, METU Teknokent, MET District, Cankaya, Ankara, Turkey

    Pelin Pocan & Haluk Hamamci

  3. Faculty of Engineering and Architecture, Konya Food and Agriculture University, Konya, Turkey

    Erinc Bahcegul

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  1. Pelin Pocan
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  2. Erinc Bahcegul
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  3. Mecit Halil Oztop
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  4. Haluk Hamamci
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Correspondence to Mecit Halil Oztop.

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Pocan, P., Bahcegul, E., Oztop, M.H. et al. Enzymatic Hydrolysis of Fruit Peels and Other Lignocellulosic Biomass as a Source of Sugar. Waste Biomass Valor 9, 929–937 (2018). https://doi.org/10.1007/s12649-017-9875-3

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  • DOI: https://doi.org/10.1007/s12649-017-9875-3

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