Abstract
Odorless hydrolyzed collagen (HC) powder from fish skin with antioxidative activity has gained increasing demand. This investigation aimed to compare the impacts of different hydrolysis processes of seabass skin without descaling as well as spray-drying conditions on characteristics and antioxidative activities of resulting HC. For one-step hydrolysis, 3% papain at 40 °C for 3 h (P) or 2% alcalase at 50 °C for 2 h (A) were used. Two-step hydrolysis, conducted as stepwise process using the combination of both processes, included P + A and A + P. P + A process rendered the HC with higher yield, hydroxyproline recovery, α-amino group content and antioxidative activities, compared to one-step process and A + P process (P < 0.05). HC powder obtained from spray-drying with inlet temperature of 200 °C showed the lower abundance of odorous compounds, compared with that using 150 °C. Nevertheless, no differences in antioxidative activity were obtained between both HCs (P > 0.05). The odorless antioxidative HC powder possessed high pH and thermal stability. Therefore, the combination of appropriate processes including hydrolysis and drying could yield the odorless antioxidative HC powder, which could widen the applications in food or pharmaceutical products.
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This work was supported by the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission. The TRF Distinguished Research Professor Grant was also acknowledged.
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Benjakul, S., Karnjanapratum, S. & Visessanguan, W. Production and Characterization of Odorless Antioxidative Hydrolyzed Collagen from Seabass (Lates calcarifer) Skin Without Descaling. Waste Biomass Valor 9, 549–559 (2018). https://doi.org/10.1007/s12649-017-0008-9
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DOI: https://doi.org/10.1007/s12649-017-0008-9