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Exploitation of Agricultural Wastes and By-Products for Production of Aureobasidium pullulans Y-2311-1 Xylanase: Screening, Bioprocess Optimization and Scale Up

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Abstract

The potential of several agricultural wastes and by-products (wheat bran, oat bran, corn cob, brewer’s spent grain, malt sprout, artichoke stem, sugar beet pulp, olive seed, cotton stalk and hazelnut skin) was examined as the substrate for xylanase production by Aureobasidium pullulans Y-2311-1. Based on the screening studies, wheat bran was selected as the best substrate for further optimization studies. The effects of initial medium pH, temperature and incubation time on xylanase production in shake flask system were optimized by response surface methodology (RSM). The optimum levels of the process variables defined by the model (initial medium pH, 4.24; temperature, 30.27 °C; and incubation time 126.67 h) resulted in production of 85.19 U/ml xylanase. Taking the RSM optimized parameters in shake-flask scale into consideration; xylanase production was scaled up to bioreactor system with a working volume of 1.5 l. The peak of enzyme production was achieved after 126 h incubation that has previously been determined by RSM studies at shake flask level. Furthermore, the optimum levels of agitation and aeration in bioreactor system was found as 200 rpm and 1.5 vvm. Maximum enzyme production was close to 85 kU/l which could be translated into a productivity of 0.68 kU/l/h. No previous work considered the statistical optimization of xylanase production by A. pullulans on wheat bran and scale up of the bioprocess to a bioreactor system.

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Acknowledgments

This work was financially supported by The Scientific and Technological Research Council of Turkey-TUBITAK (Project No: TOVAG 112O521) and Ege University Science and Technology Center-EBILTEM (Project No: 2013/BIL/025). S. Yegin is a management committee member in Cost Action TD1203 entitled as Food Waste Valorization for Sustainable Chemicals, Materials and Fuels (EUBis).

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Authors and Affiliations

  1. Department of Food Engineering, Ege University, 35100, Bornova, Izmir, Turkey

    Sirma Yegin & Yekta Goksungur

  2. Department of Food Engineering, Izmir Institute of Technology, 35430, Urla, Izmir, Turkey

    Ali Oguz Buyukkileci

  3. Department of Bioengineering, Ege University, 35100, Bornova, Izmir, Turkey

    Sayit Sargin

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  1. Sirma Yegin
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Correspondence to Sirma Yegin.

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Yegin, S., Buyukkileci, A.O., Sargin, S. et al. Exploitation of Agricultural Wastes and By-Products for Production of Aureobasidium pullulans Y-2311-1 Xylanase: Screening, Bioprocess Optimization and Scale Up. Waste Biomass Valor 8, 999–1010 (2017). https://doi.org/10.1007/s12649-016-9646-6

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