Skip to main content
SpringerLink
Log in

In vitro and in vivo anthelmintic effects of Sterospermum kunthianum (Cham-Holl) leaf extract against Ascaridia galli in experimentally infected broiler chickens

  • Original Article
  • Published:
Journal of Parasitic Diseases Aims and scope Submit manuscript

Abstract

This study was to assess the anthelminthic potential of Sterospermum kunthianum leaf extract against Ascaridia galli in experimentally infected broiler chickens. The extract and fractions were evaluated for in vitro inhibition and in vivo anthelmintic effects. Acute toxicity studies of extract revealed no sign of toxicity or death in birds at oral dose range of 1000–5000 and was considered safe. There was a concentration dependent decrease on inhibition of A. galli egg embryonation and deparasitization. At 100 mg/ml, albendazole (ALB) caused the highest inhibition of embryonation (195.3 ± 0.9) which was not significantly different from the decrease caused by crude methanol extract (CME) (188 ± 0.9), hexane fraction (HF) (177 ± 1.2) or ethyl acetate fraction (EAF) (168.3 ± 0.9). The highest inhibition rates (%) were 97, 94, 88 and 85 for ALB, CME, HF and EAF, respectively. The deparasitization obtained at day 21 in ALB (95.62%) treated birds was not significantly (P > 0.05) different from the 81.27% and 89.24% obtained from the crude methanol treated birds. The deparasitization caused by CME at 400 mg/kg (89.24%) was significantly higher than the one caused by EAF at the same dose (50.19%). Day 21 post treatment, significantly (P < 0.05) higher deparasitization was recorded for CME and HF at dosage of 400 mg/kg when compared to 200 mg/kg. Histopathology findings revealed necrosis of the mucosal gland and villi in chickens. In conclusion, the leaf extract and fractions S. kunthianum have been shown to possess anthelmintic activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Abdalla AH, Abeer EM, Rasha AHA, Enas AMH (2012) Evaluation of the ethanolic extracts of three plants for their molluscicidal activities against snails’ intermediate hosts of Schistosoma mansoni and Fasciola. Int J Basic Appl Sci 1(3):235–249

    Google Scholar 

  • Abdel Aziz AR, Mahmoud RA, Mohammad A, Mosaab AO, Khaled S (2018) In vitro and in vivo anthelmintic activity of pumpkin seeds and pomegranate peels extracts against Ascaridia galli. Beni-Suef Univ. J Basic Appl Sci. https://doi.org/10.1016/j.bjbas.2018.02.003

    Article  Google Scholar 

  • Abdulrazak LA (2015) Effects of alcoholic extract of Curcuma longai on Ascaridia infestation affecting chickens. Indian J Exp Biol 53:452–456 (PMID: 26245030)

    Google Scholar 

  • Aiswarya G, Reza KH, Radhika G, Rahul VS (2011) Study for anthelminthic activity of cashew apple (Anacardium occidentale) extract. Int J Pharm Sci Rev Res 6(1):44–47

    Google Scholar 

  • Aliyu MS, Hanwa UA, Tijjani MB, Aliyu AB, Ya’u B, (2009) Phytochemical and antibacterial properties of leaf extract of Stereospermum kunthianum (Bignoniaceae). Nig J Basic Appl Sci 17(2):235–239. https://doi.org/10.4314/njbas.v17i2.49912

    Article  Google Scholar 

  • Aristide T, Sylvin O, Mohammed BB, Adama K, Innocent PG (2017) Phytochemical analysis and ovicidal activity of Cassia sieberiana, Guiera senegalensis and Excoecaria grahamii extracts. Afr J Pharm Pharmacol 11(44):554–560. https://doi.org/10.5897/AJPP2017.4837

    Article  Google Scholar 

  • Bauri RK, Mary NT, Singray SK (2015) A review on use of medicinal plants to control parasites. Indian J Nat Prod Resour, 6(4): 268–277 Corpus ID: 40546874

  • Ching FP, Otokiti IO, Egert-Omoneukanrin B (2013) Dimethoxyflavone isolated from the stem bark of Stereospermum kunthianum possesses antidiarrhoeal activity in rodents. Afr J Tradit Complement Altern Med 10(4):47–51. https://doi.org/10.4314/ajtcam.v10i4.8

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Coles GC, Bauer C, Borgsteede FH, Geerts S, Klei TR, Taylor MA, Waller PJ (1992) World association for the advancement of veterinary parasitology (W.A.A.V.P): methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Vet Parasitol 44:35–44. https://doi.org/10.1016/0304-4017(92)90141-u

    Article  CAS  PubMed  Google Scholar 

  • Deore SL, Khadabadi SS, Kamdi KS, Ingle VP, Kawalkar NG, Sawarkar PS, Patil UA, Vyas AJ (2009) In vitro anthelmintic activity of Cassia tora. Int J ChemTech Res, 1(2): 177–179 Corpus ID: 92803070

  • Doughari JH, Human IS, Bennade S, Ndakidemi PA (2009) Phytochemicals as chemotherapeutic agents and antioxidants: possible solution to the control of antibiotic-resistant verocytotoxin producing bacteria. J Med Plants Res 3(11):839–848

    CAS  Google Scholar 

  • Fairbairn D (1970) Physiological hatching of Ascaris lumbricoides eggs. In: Experiments and Techniques in Parasitology. A.I. Mclnnis and M.Vogue Eds, Freeman, San Francisco, USA

  • Falodun A, Okpo S (2009) Evaluation of analgesic and anti-inflammatory compounds from Stereospermum kunthianum (Bignoniaceae). Int J Pharmacol Tech Res 1(4):1065–1068. https://doi.org/10.4314/tjpr.v8i6.6

    Article  Google Scholar 

  • Guèye EF (1999) Ethnoveterinary medicine against poultry diseases in African villages. World’s Poultry Sci J. https://doi.org/10.1079/WPS19990013

    Article  Google Scholar 

  • Hafiz AB, Muhammad AR, Muhammad AA, Imran AK, Abdul A, Zahid M, Shaukat HM (2015) Prevalence of Ascaridia galli in white leghorn layers and Fayoumi-Rhode Island Red crossbred flock at government poultry farm Dina, Punjab, Pakistan. Trop. Biomed., vol. 32(1): 11–16 Corpus ID: 25611801

  • Islam KR, Farjana T, Begum N, Mondal MMH (2008) In vitro efficacy of some indigenous plants on the inhibition of development of eggs of Ascaridia galli (digenia: nematoda). Bangladesh J Vet Med 6(2):159–167. https://doi.org/10.3329/bjvm.v6i2.2330

    Article  Google Scholar 

  • Katoch R, Anish Y, Godara R, Khajuria JK, Borkataki S, Sodhi SS (2012) Prevalence and impact of gastrointestinal helminths on body weight gain in backyard chickens in subtropical and humid zone of Jammu. India J Parasit Dis 36(1):49–52. https://doi.org/10.1007/s12639-011-0090-z

    Article  CAS  PubMed  Google Scholar 

  • Kessewdeg B, Basaznew B, Mersha C (2014) Study on effects and occurrence of nematodes in local and exotic chickens in and around Bahir Dar. Northwest Ethiopia Am-Eurasian J Sci Res 9(3):62–66. https://doi.org/10.5829/idosi.aejsr.2014.9.3.86148

    Article  Google Scholar 

  • Khokon JU, Sharifuzzaman SEH, Rahman MA, Kisku JJ, Mostofa M (2014) Efficacy of neen leaf extract against ascariasis in indigenous chicken. Int J Nat Soc Sci 1:25–30

  • Lukas KA, Abdu PA, Ajanusi JO, Oniye SJ, Ezealor AU (2012) Effects of Ascaridia galli infection on body weight and blood parameters of experimentally infected domestic pigeons (Columba livia domestica) in Zaria. Nigeria Revista Científica UDO Agrícola 12(4):960–964. https://doi.org/10.1016/j.jalz.2011.05.1414

    Article  Google Scholar 

  • Luna LG (1968) Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology. McGraw-Hill, New York, pp 111–112

    Google Scholar 

  • Lutoti S, Anyama NG, Iberet J, Bamuwamye M, Kwiringira W, Aber J, Nambejja C, Agwaya M, Okia C, Nambatya GK, Ogwang PE, Onegi B (2014) Evaluation of Anti-theilerial activity of a lipophilic root bark extract of Stereospermum kunthianum against Theileria parva. Int J Emerg Trends Sci Technol, 1(4): 499–508. Corpus ID: 31757882

  • Lyndem LM, Tandon V, Das B (2008) Anthelmintic efficacy of medicinal plants from Northeast India against hookworms: an in vitro study on Ancylostoma Ceylanicum. Pharmacologyonline, 3: 697–707. Corpus ID: 87035700

  • Naphade ST (2013) A survey on prevalence of helminth infection in poultry birds from Marathwada region of Maharashtra (India). Int J Res Biosci 3(1):13–18

    Google Scholar 

  • Nayak S, Sahoo AM, Chakraborti CK (2011) Phytochemical screening and anthelmintic activity study of Saraca indica leaves extracts. Int Res J Pharm 2(5):194–197

    Google Scholar 

  • Nizam I’m (2016) Efficacy of aqueous extract of Xylopia aethiopica whole fruit on experimental Ascaridia galli infection in Guinea fowl keets (Numidia meleagris galata papas). Master of Science Dissertation, Ahmadu Bello University, Zaria

  • Ohaeri CC, Okwum C (2013) Helminthic parasites of domestic fowls in Ikwuano, Abia State Nigeria. J Nat Sci Res, 3(11). Corpus ID: 4952579

  • Oloche JJ, Okwuasaba F, Obochi GO (2016) Review of phytochemical, pharmacological and toxicological profile of Stereospermum kunthianum. J Adv Med Pharm Sci 5(1):1–10. https://doi.org/10.9734/JAMPS/2016/21743

    Article  Google Scholar 

  • Onyilofe SE, Musa MS, Joseph OA, Suleiman FA (2015) In vitro anthelmintic efficacy of extracts of Citrus aurantifolia (Christm) swingles fruit peels against Heligmosomoides bakeri ova and larvae. Int. J Curr Pharm Res, 7(2): 92–96. Corpus ID: 12192111

  • Salam ST (2015) Ascariasis in backyard chicken–prevalence, pathology and control. Int J Recent Sci Res 6(4):3361–3365. https://doi.org/10.24327/IJRSR

    Article  Google Scholar 

  • Saljaba GM, Osemeahon SA, Baminas JT (2015) Photochemical and antimicrobial activities of stem-bark of Stereospermum kunthianum plant. Int J Res 2:752–758

    Google Scholar 

  • Suleiman MM, Mohammed M, Yusuf OA, Ajanusi JO (2005) Anthelmintic activity of the crude methanol extract of Xylopia aethiopica against Nippostrongylus brasiliensis in rats. Vet Arh, vol. 75(6): 487–495 Corpus ID: 17540907

  • Tor-Anyiin TA, Anyam JV (2013) Phytochemical evaluation and antibacterial activity: a comparison of various extracts from some Nigerian trees. Peak J Med Plant Res 1(2):13–18

    Google Scholar 

  • Treas, Evans (1989) Trease and Evans Pharmacognosy, 12th edn. Billiere Tindall, Edinborough, London. Pp.546–547.

  • Umar AH (2016) Phytochemical, antihypertensive and antimicrobial studies on the stem bark extract of Stereospermum kunthianum Cham (Bignoniaceae). Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, PhD thesis, Ahmadu Bello University Zaria, Nigeria

  • Waterman C, Smith RA, Pontiggia L, Dermarderosian A (2010) Anthelmintic screening of Sub-Saharan African plants used in traditional medicine. J Ethnopharmacol 127(3):755–759. https://doi.org/10.1016/j.jep.2009.11.025

    Article  PubMed  Google Scholar 

  • Yusuf KH (2010) Ascaridia galli in birds: Prevalence and comparative effect of oral and parental ivermectin in two breeds of broilers. Dissertation, Ahmadu Bello University

Download references

Acknowledgements

The staff of the laboratory of the Department of Veterinary Pharmacology and Toxicology, and Department of Parasitology and Entomology, Faculty of Veterinary Medicine and well as the laboratory staff of the Department of Pharmacognosy and Drug Development, Faculty of Pharmaceutical Sciences and also late Mr. Bulus of the Department of Veterinary Microbiology, all within Ahmadu Bello University, Zaria.

Author information

Authors and Affiliations

  1. Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Kaduna State, Nigeria

    Kyari Stephen, Ologunja Joseph Ajanusi & Matthew Ogwiji

  2. Department of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Kaduna State, Nigeria

    Mohammed Musa Suleiman

  3. Department of Veterinary Pathology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Kaduna State, Nigeria

    Ochuko Orakpoghenor

  4. Department of Zoology, Faculty of Life Sciences, Ahmadu Bello University, Zaria, Kaduna State, Nigeria

    Kyari Stephen

Authors
  1. Kyari Stephen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ologunja Joseph Ajanusi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mohammed Musa Suleiman
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ochuko Orakpoghenor
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Matthew Ogwiji
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Kyari S, Ajanusi O.J and Suleiman M.M designed the experiment, contributed in the discussion, Orakpoghenor O read and interpreted the histopathological slides while Ogwiji M contributed in the statistical analysis.

Corresponding author

Correspondence to Kyari Stephen.

Ethics declarations

Conflict of interest

The authors declare no conflict of interest.

Ethical approval

This was gotten from the Ahmadu Bello University Committee on Animal Use and care (ABUCAUC).

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Stephen, K., Ajanusi, O.J., Suleiman, M.M. et al. In vitro and in vivo anthelmintic effects of Sterospermum kunthianum (Cham-Holl) leaf extract against Ascaridia galli in experimentally infected broiler chickens. J Parasit Dis 46, 152–158 (2022). https://doi.org/10.1007/s12639-021-01426-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12639-021-01426-6

Keywords

Search

Navigation