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Effect of phenyl vinyl sulphone cysteine protease inhibitor on Schistosoma mansoni: in vitro and in vivo experimental studies

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Abstract

The present work aimed to study the effect of phenyl vinyl sulphone (PVS), a CPI, on different stages of Schistosoma (S.) mansoni in an in vitro culture study and in experimentally infected mice, compared to PZQ. As regards the in vitro study, different concentrations of PVS (1, 2, 4, 6, 8 and 10 µg/ml) and PZQ (1 µg/ml) were assessed by % worm mortality for schistosomula and adults, and hemoglobin degradation by schistosomula. In vivo study included 8 groups of mice. Intraperitoneal PVS, subgroup (a), and oral PZQ, subgroup (b), were assessed at different durations post infection (pi); at 1, 3, 5 and 7 weeks pi (groups I, II, III and IV, respectively). Infection, PVS, PZQ, and normal control groups (groups V–VIII) were included. The anti-schistosomal effects of PVS were assessed by parasitological, histopathological and haematological parameters. In in vitro study, PVS had a schistosomicidal effect in a concentration and time dependent manner, PVS showed 100% schistosomula mortality at day 2 and 92% adult worm mortality at day 5. Furthermore, PVS decreased hemoglobin degradation by schistosomula. In in vivo study, PVS showed a decrease in total worm burden and tissue egg load in intestine and liver with an increase in number of dead ova in intestine of mice. Furthermore, PVS resulted in a decrease in number, size and cellularity of hepatic granulomas and an increase in hemoglobin concentration.PVS was better than PZQ in reducing each of tissue egg count in intestine at 5 and 7 weeks pi, and hepatic granuloma size at 3, 5 and 7 weeks pi. These results suggest that PVS can be a promising chemotherapeutic agent in Schistosoma mansoni infection.

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Authors and Affiliations

  1. Faculty of Medicine, Ain-Shams University, Ramsis St., Abbassia, Cairo, 11566, Egypt

    Manal Salah El-Din Mahmoud, Ayman Nabil Ibrahim, Abeer Fathy Badawy & Nourhan Mohamed Abdelmoniem

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  1. Manal Salah El-Din Mahmoud
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  2. Ayman Nabil Ibrahim
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  3. Abeer Fathy Badawy
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  4. Nourhan Mohamed Abdelmoniem
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Correspondence to Ayman Nabil Ibrahim.

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We wish to confirm that there are no known conflicts of interest associated with this publication and there has been no significant financial support for this work that could have influenced its outcome. We confirm that the manuscript has been read and approved by all named authors and that there are no other persons who satisfied the criteria for authorship but are not listed. We further confirm that the order of authors listed in the manuscript has been approved by all of us.

Ethical approval

The study was approved by the Research Ethics Committee, Faculty of Medicine, Ain Shams University. All the animal experiments were performed according to the rules and regulation of the Animal Ethics rules, Ain-Shams University, Cairo, Egypt.

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Mahmoud, M.S.ED., Ibrahim, A.N., Badawy, A.F. et al. Effect of phenyl vinyl sulphone cysteine protease inhibitor on Schistosoma mansoni: in vitro and in vivo experimental studies. J Parasit Dis 41, 1049–1058 (2017). https://doi.org/10.1007/s12639-017-0933-3

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  • DOI: https://doi.org/10.1007/s12639-017-0933-3

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