Skip to main content
SpringerLink
Log in

Molecular characterization of Leishmania parasites isolated from sandflies species of a zoonotic cutaneous leishmaniasis in Musiyan south west Iran

  • Original Article
  • Published:
Journal of Parasitic Diseases Aims and scope Submit manuscript

Abstract

Cutaneous leishmaniasis (CL) is vector borne parasitic disease, considered as public health problem especially in border of Iran and Iraq, Dehloran County (Musian district). The aim of this study was molecular identification of Leishmania parasites in sandfly as vectors of Leishmaniasis. Totally 280 female sandflies were trapped by sticky traps from 7 rural areas of Musiyan in September–November 2012. All sandflies were identified using morphological characters of the head and abdominal terminalia. DNA was extracted from female sandflies and Leishmania was identified using PCR and sequencing. All 280 trapped sandflies were identified as Phelobotumus Papatasi and Leishmania infections were detected in 3.2 % out of 280 female sandflies. All leishmania were identified as L. major and submitted in Gene bank as: LC014642.1, LC014641.1, LC014640.1 and LC014639.1. Frequency of Phlebotomus Papatasi and infection with L. major in studied regions showed that this vector is dominant in these areas.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Davami MH, Motazedian MH, Sarkari B (2010) The changing profile of cutaneous Leishmaniasis in a focus of the disease in Jahrom district, southern Iran. Ann Trop Med Parasitol 104:377–382

    Article  CAS  PubMed  Google Scholar 

  • Desjeux P (1991) Information on the epidemiology and control of the Leishmaniasis by country or territory, 2nd edn. WHO/Leish/91.30, WHO, Geneva

  • Hajjaran H et al (2013) Molecular identification and polymorphism determination of cutaneous and visceral leishmaniasis agents isolated from human and animal hosts in Iran. Hindawi Publishing Corporation BioMed Research International, Volume 2013 Article ID 789326, p 7

  • Hajjaran H, Mohebali M, Alimoradi S, Abaei MR, Edrissian GhH (2009) Isolation and characterization of pathogenic Leishmania turanica from Nesokia indica (Rodentia, Muridae) by PCR-RFLP and ITS1 sequencingin Iran. Trans R Soc Trop Med Hyg 103:1177–1179

    Article  CAS  PubMed  Google Scholar 

  • Islamic Republic of Iran Ministry of Heath and Medical Education (2007) [Instruction of Leishmaniasis control]. Tehran: Center for disease control 2007:78(Persian)

  • Javadian E, Mesghali A (1974) Studies on cutaneous leishmaniasis in Khuzestan, Iran. Part I. The leptomonad infection of sandflies. Bull Soc Pathol Exot 67:513–516

    CAS  Google Scholar 

  • Javadian E, Tesh R, Saidi S, Nadim A (1977) Studies on the epidemiology of sandfly fever in Iran. III. Host-feeding patterns of Phlebotomus papatasi in an endemic area of the disease. Am J Trop Med Hyg 26:294–302

    CAS  PubMed  Google Scholar 

  • Javadian E, Jalali-Galousang A, Seyedi Rashti MA (1997) Sandflies of Ilam province, west of Iran with description of two new species from the Genus Phlebotomus: Ph. ilami and Ph. nadimi I. Iran J Public Health 26:13–20

    Google Scholar 

  • Kavarizadeh F, Vazirianzadeh B, Rassi Y, Jalali Glusang A, Moravvej SA (2013) A faunistic study of sandflies of Musian district southwestern of Iran. Pak J Zool 45:549–554

    Google Scholar 

  • Killick-Kendrick R (1990) Phlebotomine vectors of the leishmaniasis: a review. Med Vet Entomol 4:1–24

    Article  CAS  PubMed  Google Scholar 

  • Killick-Kendrick R (1999) The biology and control of phlebotominae sandflies. Med Vet Entomol 17:279–289

    CAS  Google Scholar 

  • Lewis DJ (1982) A taxonomic review of the genus Phlebotomus (Diptera: Psychodidae). Bull Br Mus Nat Hist Entomol Ser 45:121–209

    Google Scholar 

  • Mansoori AM, Kafravi G, Sharifim (2009) Geography of Ilam province, th edn. Company of Iran Text Book Printing and Publishing, Tehran

    Google Scholar 

  • Mirzaei A, Rouhani S, Taherkhani H, Farahmand M, Kazemi B, Hedayati M et al (2011) Detection and isolation of Leishmania species in naturally infected Rhombomis opimus, are reservoir host of zoonotic cutaneous leishmaniasis in Turkemen Sahara, north east of Iran. Exp Parasitol 29:375–380

    Article  Google Scholar 

  • Muller GC, Schlein Y (2011) Different methods of using attractive sugar baits (ATSB) for the control of Phlebotomus Papatasi. Ann Trop Med Parasitol 36:64–70

    Google Scholar 

  • Nadim A, Javadian E (1976) Key for the species identification of sandflies (Diptera Phlebotominae) of Iran. Iran J Public Health 5:25–28

    Google Scholar 

  • Nadim A, Seyedi-Rashti MA (1971) Brief review of the epidemiology of various types of leishmaniasis in Iran. Acta Med Iran 14:99–106

    Google Scholar 

  • Nadim A, Seyedi-Rashti MA, Mesgali A (1968a) Epidemiology of cutaneous leishmaniasis in Turkemen Sahara Iran. J Trop Med Hyg 71:238–239

    CAS  PubMed  Google Scholar 

  • Nadim A, Mesghali A, Amini H (1968b) Epidemiology of cutaneous leishmaniasis in Isfahan province of Iran. III. The vector. Trans R Soc Trop Med Hyg 62:543–549

    Article  CAS  PubMed  Google Scholar 

  • Noyes HA, Reyburn H, Bailey JW, Smith D (1998) A nested PCR-based schizodeme method for identifying Leishmania kinetoplast minicircle classes directly from clinical samples and its application to the study of the epidemiology of Leishmania tropica in Pakistan. J Clin Microbiol 36:2877–2881

    CAS  PubMed  PubMed Central  Google Scholar 

  • Parvizi P, Mauricio I, Aransay AM, Miles MA, Ready PD (2005) First detection of Leishmania major in peri domestic Phlebotomus Papatasi from Isfahan province, Iran: comparison of nested PCR of nuclear ITS ribosomal DNA and semi-nested PCR of minicircle kinetoplast DNA. Acta Trop 93:75–83

    Article  CAS  PubMed  Google Scholar 

  • RanjbarKermani M (1989) Evaluating the status of cutaneous leishmaniasis among fighters and determining its main vector in north-western Khuzestan and Dehloran. Thesis for receiving a Master’s degree in Medical Entomology and Vector Control, School of Public Health, Tehran University No. 1690

  • Rassi Y, Gassmi MM, Javadian F, Motazedian MH (2005) Confirmation of Phlebotomus papatasi as the main vector of cutaneous leishmaniasis in south of Iran of the third proceeding of the international symposium on phlebotomine sandflies, Tunis, Tunisia, Archives de L Institut Pasteur de Tunis 82:43

  • Roshan-Ghalb M, Parvizi P (2012) Isolation and determination of Leishmania major and Leishmania turanica in Phlebotomus papatasi main vector of zoonotic cutaneous leishmaniasis in Turkenmen Sahara, Golestan Province. J Mazand Univ Med Sci 21:74–83 (Persian)

    Google Scholar 

  • Seydei-Rashti MA, Nadim A (1992) The genus Phlebotomus (Diptera: Psychodidae, Phlebotominae) of the countries of the eastern Mediterranean region. Iran J Public Health 21:11–50

    Google Scholar 

  • Sharbatkhori M, Spotin A, Taherkhani H, Roshanghalb M, Parvizi P (2014) Molecular variation in Leishmania parasites from sandflies species of a zoonotic cutaneous leishmaniasis in northeast of Iran. J Vector Borne Dis 51:16–21

    CAS  PubMed  Google Scholar 

  • Strelkova MV, Eliseev LN, Ponirovsky EN, Dergacheva TI, Evans DA (2001) Mixed Leishmania infections in Rhombomys opimus: a key to the persistence of Leishmania major from one transmission season to the next. Ann Trop Med Parasitol 95:811–819

    CAS  PubMed  Google Scholar 

  • Tashakori M, Kuhls K, Al-Jawabreh A, Mauricio I, Schönian G, Farajnia S et al (2006) Leishmania major: genetic heterogeneity of Iranian isolates by single-strand conformation polymorphism and sequence analysis of ribosomal DNA internal transcribed spacer. Acta Trop 98:52–58

    Article  CAS  PubMed  Google Scholar 

  • WHO (2010) Control of the leishmaniases. Report of a meeting of the WHO Expert Committee Leishmaniases, on the Control of Geneva 949:22–26

  • World Health Organization (1993) Control of tropical diseases, the leishmaniasis. WHO/CTD/ICO, Geneva, pp 2–14

    Google Scholar 

  • Yaghoobi-Ershadi MR (2012) Phlebotomine sandflies (Diptera: Psychodidae) in Iran and their role on Leishmania transmission. J Arthropod-Borne Dis 6:1–17

    PubMed  PubMed Central  Google Scholar 

  • Yaghoobi-Ershadi MR, Javadian E (1996a) Seasonal variation of Leishmania major infection rates in sandflies from rodent burrows in Isfahan province. Iran Med Vet Entomol 10:181–184

    Article  CAS  PubMed  Google Scholar 

  • Yaghoobi-Ershadi MR, Javadian E (1996b) Epidemiological study of reservoir hosts in an endemic area of zoonotic cutaneous leishmaniasis in Iran. Bull World Health Org 74:587–590

    CAS  PubMed  PubMed Central  Google Scholar 

  • Yaghoobi-Ershadi MR, Javadian E, Kannani A (1995) Host preference pattern of phlebotominae sandflies of Borkhar rural district, Isfahan province, Iran. Acta Trop 60:155–158

    Article  CAS  PubMed  Google Scholar 

  • Yaghoobi-Ershadi MR, Akhavan AA, Mohebali M (1996) Meriones libycus and Rhombomys opimus (Rodentia: Gerbillidae) are the main reservoir hosts in a new focus of zoonotic cutaneous leishmaniasis in Iran. Trans R Soc Trop Med Hyg 90:503–504

    Article  CAS  PubMed  Google Scholar 

  • Yaghoobi-Ershadi MR, Akhavan AA, Zahraei-Ramezani AR, Javadian E, Motavalli-Emami M (2000) Field trial for the control of zoonotic cutaneous leishmaniasis in Badrood, Iran. Ann Saud Med 20:386–389

    CAS  Google Scholar 

Download references

Acknowledgments

This study was financially supported by grants No: 91114 from Ahvaz Jundishapur Medical Sciences. The authors would like to thank Mr. Akhavan A. A. of Tehran University and health centers of Musian and Dasht Abbas for their help in sample collection.

Author information

Authors and Affiliations

  1. Department of Medical Parasitology and Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Farzaneh Kavarizadeh, Shahram Khademvatan, Mohammad Hossein Feizhaddad & Mehdi Zarean

  2. Department of Medical Parasitology and Mycology and Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran

    Shahram Khademvatan

  3. Department of Medical Entomology and Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Babak Vazirianzadeh

Authors
  1. Farzaneh Kavarizadeh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shahram Khademvatan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Babak Vazirianzadeh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mohammad Hossein Feizhaddad
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mehdi Zarean
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shahram Khademvatan.

Ethics declarations

Conflict of interest

The authors declare no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kavarizadeh, F., Khademvatan, S., Vazirianzadeh, B. et al. Molecular characterization of Leishmania parasites isolated from sandflies species of a zoonotic cutaneous leishmaniasis in Musiyan south west Iran. J Parasit Dis 41, 274–281 (2017). https://doi.org/10.1007/s12639-016-0792-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12639-016-0792-3

Keywords

Search

Navigation