Abstract
Ticks and tick-borne diseases are the main problems affecting the livestock production in Egypt. Bovine babesiosis has adverse effects on the animal health and production. A comparison of Giemsa stained blood smears, polymerase chain reaction (PCR) and nested PCR (nPCR) assays for detection of Babesia bovis infection in Egyptian Baladi cattle (Bos taurus) in reference to reverse line blot was carried out. The sensitivity of PCR and nested PCR (nPCR) assays were 65 and 100 % respectively. Giemsa stained blood smears showed the lowest sensitivity (30 %). According to these results using of PCR and nPCR target for B. bovis, [BBOV-IV005650 (BV5650)] gene are suitable for diagnosis of B. bovis infection. The 18Ss rRNA partial sequence confirmed that all the positive samples were Babesia bovis and all of them were deposited in the GenBank databases (Accession No: KM455548, KM455549 and KM455550).
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References
AbouLaila M, Yokoyama N, Igarashi I (2010a) Development and evaluation of a nested PCR based on spherical body protein 2 genes for the diagnosis of Babesia bovis infection. Vet Parasitol 139:45–50
AbouLaila M, Yokoyama N, Igarashi I (2010b) Development and evaluation of two nested PCR assays for the detection of Babesia bovis from cattle blood. Vet Parasitol 172:65–70
Bose R, Jorgensen WK, Dalgliesh RJ, Friedhoff KT, De vos AJ (1995) Current state and future trends in the diagnosis of babesiosis. Vet Parasitol 57:61–67
Coles EH (1986) Veterinary clinical pathology, 4th edn. W B Saunders Company, London and Toronto Philadelphia, pp 46–56
Costa-Junior LM, Rabelo EML, Filho OAM, Ribeiro MFB (2006) Comparison of different direct diagnostic methods to identify Babesia bovis and Babesia bigemina in animals vaccinated with live attenuated parasites. Vet Parasitol 139:231–236
El-Ashker M, Hotzel H, Gwida M, El-Beskawy M, Silaghi C, Tomaso H (2015) Molecular biological identification of Babesia, Theileria, and Anaplasma species in cattle in Egypt using PCR assays, gene sequence analysis and a novel DNA microarray. Vet Parasitol 207:329–334
Elsify A, Sivakumar T, Nayel M, Salama A, Elkhtam A, Rizk M, Mosaab O, Sultan K, Elsayed S, Igarashi I, Yokoyama N (2015) An epidemiological survey of bovine Babesia and Theileria parasites in cattle, buffaloes, and sheep in Egypt. Parasitol Int 64:79–85
Figueroa JV, Chieves LP, Johnson GS, Buening GM (1993) Multiplex polymerase chain reaction based assay for the detection of Babesia bigemina Babesia bovis and Anaplasma marginale DNA in bovine blood. Vet Parasitol 50:69–81
Gubbels JM, de Vos AP, van der Weide M, Viseras J, Schouls LM, de Vries E, Jongejan F (1999) Simultaneous detection of bovine Theileria and Babesia species by reverse line blot hybridization. J Clin Microbiol 37:1782–1789
Ibrahim HM, Adjou Moumouni PF, Mohammed-Geba K, Sheir SK, Hashem IS, Cao S (2013) Molecular and serological prevalence of Babesia bigemina and Babesia bovis in cattle and water buffalos under small-scale dairy farming in Beheira and Faiyum Provinces, Egypt. Vet Parasitol 198:187–192
Mahoney DF (1969) Bovine babesiosis: a study of factors concerned in transmission. Ann Trop Parasitol 63:1–14
Martins TM, Pedro OC, Caldeira RA, do Rosario VE, Neves L, Domingos A (2008) Detection of bovine babesiosis in Mozambique by a novel seminested hot-start PCR method. Vet Parasitol 153:225–230
McCosker PJ (1981) The global importance of babesiosis. In: Ristic M, Krier JP (eds) babesiosis. Academic Press, New York, pp 1–24
Mosqueda J, Olvera-Ramírez A, Aguilar-Tipacamú G, Cantó GL (2012) Current Advances in Detection and Treatment of Babesiosis. Curr Med Chem 19:1504–1518
Nagati HE (1947) Some new and rare records of piroplasmosis with a list of the species of Babesia and Theileria so far recorded from Egypt. Vet Rec 59:145–147
Oliveira-Sequeira TC, Oliveira MC, Araujo JP, Amarante AF (2005) PCR-based detection of Babesia bovis and Babesia bigemina in their natural host Boophilus microplus and cattle. Int J Parasitol 35:105–111
Radostits OM, Gay CC, Blood DC, Hinchcliff KW (2000) A text book of veterinary medicine, 9th edn. Baieller, Tindal and Cox, pp 1328–1329
Radwan AM, Ibrahim MA, Abdalla AA, AL-Hosary AA, Ahmed LS (2013) Conventional detection of Babesia bovis in tick infested cattle and its effect on the hematological profile. In: 16th ISAH congress. Nanjing, China, 5–9 May 2013, pp 170–172
Thrusfield M (2005) Veterinary epidemiology, 3rd edn. Blackwell science publishing, New Jersey, pp 313–317
Acknowledgements
This work was supported by DFG project “Molecular epidemiology network for promotion and support of delivery of life vaccines against Theileria parva and Theileria annulata infection in Eastern and Northern Africa” (DFG project SE862/2-1).
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AL-Hosary, A.A.T. Comparison between conventional and molecular methods for diagnosis of bovine babesiosis (Babesia bovis infection) in tick infested cattle in upper Egypt. J Parasit Dis 41, 243–246 (2017). https://doi.org/10.1007/s12639-016-0785-2
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DOI: https://doi.org/10.1007/s12639-016-0785-2