Abstract
Cryptosporidium parvum is a protozoan parasite which causes diarrheal in human and animals worldwide. Infection transmission has reported through oral-fecal by infectious objects through foods and drinks. In this study we explored the immune response pathway in animal model for C. parvum to develop the new treatment way. Oocysts collected from fecal positive for C. parvum and diluted about 1:5 in sucrose solution. New born BALB/c mice (3 days) divided to 2 different groups. Control group hadn’t received any oocyst, the test groups received 5 × 105 oocysts. 5 mice selected for each control group and 11 mice chosen for each test group. Blood collected from heart bleeds in days of 6, 9, 12 and 16. Protein concentrations determined by bio-photometer. Dot blotting used to find out total antibody concentrations oocyst antigen. Among the test and the control groups, blots appeared in test group which means antibody production, but not any blot observed in the control groups. The non-characteristic proteins in serum were measured by the biophotometer. In this study, we investigated antibody serum production against C. parvum oocysts in new born BALB/c mice. The detected antibody through dot blot technique was our aims which had conjugated to our characteristic antiserum. The recorded numbers for the controls by biophotometer related to the non characteristic proteins in serum. The results of this study can used to produce polyclonal or monoclonal antibodies against cryptosporidiosis.
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Acknowledgments
The authors express appreciation to the deputy of Department of Medical Parasitology, Shahid Beheshti University of Medical Sciences, and Tehran, Iran for the financial support of this study. Sincere thanks are extended to Prof. N. Mosafa for their kindly guides and Prof. B. Kazemi for their valuable technical support.
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Ahmadian, N., Pashaei-Asl, R., Ahmadian, M. et al. Immune response of newborn BALB/c mice to Cryptosporidium infection. J Parasit Dis 40, 1066–1070 (2016). https://doi.org/10.1007/s12639-014-0635-z
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DOI: https://doi.org/10.1007/s12639-014-0635-z