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Diagnosis of Trypanosoma evansi in bovines by indirect ELISA

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Abstract

The diagnosis of Trypanosoma evansi in animals with low parasitaemia is hampered by low diagnostic sensitivity of traditional detection methods. The present study was undertaken with an objective to improve the diagnostic tools for detection of antibodies against T. evansi infection using indirect enzyme-linked immunosorbent assay (ELISA) in bovines. The optimum concentration of antigen, test sera and conjugate were determined as 5 µg per well, 1:10 and 1: 6,000 dilutions, respectively. Among 320 cattle and 382 buffaloes examined in different parts of Rayalaseema region of Andhra Pradesh for T. evansi infection, 36.12 and 31.87 percent were found positive by indirect ELISA, respectively.

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Acknowledgments

The authors acknowledge the authorities of Sri Venkateswara Veterinary University, Tirupati for providing facilities to carry out this research. Authors are also thankful to the field veterinarians who assisted in collection of blood samples.

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Authors and Affiliations

  1. Department of Veterinary Parasitology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati, 517502, Andhra Pradesh, India

    S. Sivajothi, V. C. Rayulu, P. Malakondaiah & D. Sreenivasulu

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  1. S. Sivajothi
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  2. V. C. Rayulu
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  3. P. Malakondaiah
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  4. D. Sreenivasulu
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Correspondence to S. Sivajothi.

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Sivajothi, S., Rayulu, V.C., Malakondaiah, P. et al. Diagnosis of Trypanosoma evansi in bovines by indirect ELISA. J Parasit Dis 40, 141–144 (2016). https://doi.org/10.1007/s12639-014-0465-z

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  • DOI: https://doi.org/10.1007/s12639-014-0465-z

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