Abstract
In order to prevent the growth of pathogens in food, bacteriocins produced by various probiotic lactic acid bacteria have been recognized as potential substitutes of chemical preservatives. In this study, enterocin LD3 was purified from the cell-free supernatant of a food isolate, Enterococcus hirae LD3 using multistep chromatography. In the fruit juice, lethal concentration (LC50) of enterocin LD3 was found to be 260 µg/mL against Salmonella enterica subsp. enterica serovar Typhimurium ATCC 13311. The cells treated with enterocin LD3 were red colour indicating dead cells after propidium iodide staining, while untreated cells were found blue after staining with 4', 6-diamidino-2-phenylindole. The mechanism of cell killing was analyzed using infrared spectrum of cells treated with enterocin LD3 which was found altered in the range of 1,094.30 and 1,451.82 cm−1 corresponding to nucleic acids and phospholipids, respectively. The morphology of target cells were severely ruptured and lysed as observed under electron microscopy. Thus, the present study suggested that enterocin LD3 showed bactericidal activity against Salm. enterica subsp. enterica serovar Typhimurium ATCC 13311 and may be applied as a bio-preservative for the safety of fruit juices.
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Acknowledgements
The Department of Genetics at M. D. University Rohtak provided support for MKY and IK through its University Research Scholarship.
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The Department of Biotechnology (BT/PR8911/NDB/39/423/2013 and BT/PR8306/PID/6/738/2013), Indian Council of Medical Research (5/9/1117/2013-NUT), New Delhi, India and University Grants Commission-Scheme for Trans-disciplinary Research for India’s Developing Economy Component-1 (UGC-STRIDE-1), New Delhi, India [F.2–16/2019 (STRIDE 1)] provided financial support for this work.
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The experiments, data collection and analysis and designed the manuscript were performed by PS and MKY. The manuscript was formatted by IK. SKT, as corresponding authors, provided all laboratory facilities and chemicals and reviewed the manuscript. All authors read and approved the final version of the manuscript.
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Sheoran, P., Yadav, M.K., Kumari, I. et al. Enterocin LD3 from Enterococcus hirae LD3 Inhibits the Growth of Salmonella enterica subsp. enterica serovar Typhimurium ATCC 13311 in Fruit Juice. Probiotics & Antimicro. Prot. (2023). https://doi.org/10.1007/s12602-023-10108-z
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DOI: https://doi.org/10.1007/s12602-023-10108-z