Abstract
In this study, five strains of lactic acid bacteria (LAB) with excellent cholesterol-lowering ability were screened from fermented foods. The gastrointestinal stress resistance, intestinal adhesion, and bacteriostasis abilities were evaluated to obtain the best LAB. And then, high-cholesterol HepG2 cell model was further prepared to explore the cholesterol-lowering mechanism of the LAB. Finally, pH-sensitive hydrogel prepared by Millettia speciosa Champ. carboxymethyl cellulose and Millettia speciosa Champ. cellulose was first applied to the microencapsulation of LAB. As a result, Lactobacillus paracasei BY2 (LP-BY2) exhibited higher cholesterol-lowering activity, intestinal adhesion, and bacteriostasis abilities compared with other LAB. Furthermore, it was found that LP-BY2 could reduce the cholesterol level by regulating the expression of key genes that involved in cholesterol synthesis (HMGCR and SREBP-2), uptake (LDLR), and outflow (LXR-α, ABCA1, ABCG5, ABCG8, and CYP7A1) in liver. At the same time, microencapsulation significantly enhanced the survival rate and cholesterol-lowering ability of LP-BY2 after gastrointestinal digestion. This study will provide an available reference for the application of Lactobacillus in prevention and treatment of hypercholesterolemia.
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The data of this study is available upon request from the corresponding author.
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Funding
This study received financial support from the National Natural Science Foundation of China (22078111, 21878105), the Key Research and Development Program of Guangdong Province (2019B020213001) and the Fundamental Research Funds for the Central Universities (No.2022ZYGXZR102).
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Hua-Ling Cui supported the investigation, methodology, data analysis, and writing—original draft. Meng-Fan Li supported the investigation, methodology, and writing—reviewing and editing. Sheng-Ya Liu focused on dada analysis. Pro. Ming Yu is project administrator, who contributed to the writing—reviewing and editing. Prof. Dr. Wen-Yong Lou is the supervision and project administrator of this study. He is the financial supporter and focused on writing—reviewing and editing of this manuscript.
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Cui, HL., Li, MF., Liu, SY. et al. Regulation of Hepatocellular Cholesterol Metabolism By Lactobacillus Paracasei BY2 and Its Embedding Delivery. Probiotics & Antimicro. Prot. 16, 181–195 (2024). https://doi.org/10.1007/s12602-022-10023-9
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DOI: https://doi.org/10.1007/s12602-022-10023-9