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Lacticaseibacillus casei LH23 Suppressed HPV Gene Expression and Inhibited Cervical Cancer Cells

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Abstract

Microbiota of lower female reproductive tract is special in its microorganism composition with Lactobacillus as the predominant bacteria. A few of Lactobacillus species have been identified to benefit the inhibition of inflammatory and malignant diseases. Lacticaseibacillus casei LH23 is a strain isolated from traditional fermented food and had been demonstrate to ameliorate DSS-induced colitis in mice. In the present study, effects of Lacticaseibacillus casei LH23 on cervical cancer cells were investigated. Supernatants of lysates and heat-inactivated Lacticaseibacillus casei LH23 were found to inhibit the expression of human papillomavirus genes E6/E7 which is the main causative factor of cervical cancer. With MTT, EdU staining, and TUNEL staining assays, Lacticaseibacillus casei LH23 was shown to suppress the proliferation and induced the apoptosis of cervical cancer cells. Additionally, with wound-healing and Western-blot assays, Lacticaseibacillus casei LH23 was shown to slowdown the migration of cervical cancer cells and altered the expression of metastasis-related genes. These results demonstrated the anti-cervical cancer potential of Lacticaseibacillus casei LH23.

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All data generated during this study are included in this published article. Materials are available upon reasonable request.

Abbreviations

HPV:

Human papillomavirus

LAB :

Lactic acid bacteria

EMT:

Epithelial to mesenchymal transition

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Funding

This work was supported by the National Key R & D Program of China (2017YFD0400304) and the Natural Science Foundation of Tianjin (18JCYBJC91500).

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Author notes

  1. Shiyue Hu and Yunpeng Hao contributed equally to this paper.

Authors and Affiliations

  1. Key Laboratory of Industrial Microbiology, Ministry of Education and Tianjin City, State Key Laboratory of Food Nutrition and Safety, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, People’s Republic of China, 300457

    Shiyue Hu, Yunpeng Hao, Xiao Zhang, Yaping Yang, Meiling Liu, Nan Wang, Tong-Cun Zhang & Hongpeng He

  2. College of Life Sciences, Wuhan University of Science and Technology, Wuhan, People’s Republic of China, 430081

    Tong-Cun Zhang

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  1. Shiyue Hu
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Correspondence to Hongpeng He.

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Hu, S., Hao, Y., Zhang, X. et al. Lacticaseibacillus casei LH23 Suppressed HPV Gene Expression and Inhibited Cervical Cancer Cells. Probiotics & Antimicro. Prot. 15, 443–450 (2023). https://doi.org/10.1007/s12602-021-09848-7

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