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Oral Administration of Live and Dead Cells of Lactobacillus sakei proBio65 Alleviated Atopic Dermatitis in Children and Adolescents: a Randomized, Double-Blind, and Placebo-Controlled Study

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Abstract

Several studies suggest that probiotics might be useful in the management of atopic dermatitis (AD). However, the efficacy and comparison between both the administration of viable and non-viable probiotics on alleviation of AD is not well studied. Therefore, the purpose of this study was to evaluate the effect of L. sakei proBio65 live and dead cells when administered (1 × 1010 cells/day) for 12 weeks to children and adolescents (aged 3 to 18) with atopic dermatitis. In this randomized double-blind, placebo-controlled study, ninety patients were recruited and randomly allocated to either the L. sakei proBio65 live cells, L. sakei proBio65 dead cells, or placebo groups. Assessment of efficacy was based on the change in SCORing Atopic Dermatitis (SCORAD) score, Investigators Global Assessment (IGA) score, serum inflammatory markers such as the serum eosinophil (count), IgE, eosinophil cationic protein (ECP), CCL17 (thymus and activation-regulated chemokine [TARC]), and CCL27 (cutaneous T cell–attracting chemokine [CTACK]), and changes in skin condition (moisture and sebum) at baseline, week 6 and week 12. The SCORAD total score decreased in the live cells (p = 0.0015) and dead cell group (p = 0.0017) from the baseline after 12 weeks, whereas there were no significant changes in the placebo group when compared with baseline. The skin sebum content increased in both the live cell (p < 0.0001) and the dead cell group (p < 0.0001), suggesting potential improvements in skin barrier functions. Current data suggested a positive improvement in alleviation of AD symptoms upon oral administration of L. sakei proBio65 in both viable and non-viable forms.

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Funding

This work was supported by the 2015 Yeungnam University Research Grant.

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Authors and Affiliations

  1. College of Life and Applied Sciences, Yeungnam University, Gyeongsan, South Korea

    Irfan A. Rather, Lee-Ching Lew, Gyeong-Jun Nam, Rajib Majumder & Yong-Ha Park

  2. Department of Biological Sciences, King Abdulaziz University, Jeddah, Saudi Arabia

    Irfan A. Rather

  3. Probionic Corporation, Jeonbuk Institute for Food-Bioindustry, Jeonju, South Korea

    Byung-Chun Kim, Jong Hwan Lee & Yong-Ha Park

  4. Hansung Food Co., Ltd, Bucheon, South Korea

    Seong-Kwan Cha

  5. National Science Museum, Ministry of Science, ICT and Future Planning, Daejeon, South Korea

    Jeongheui Lim

  6. Oracle Dermatologic Clinic, Sejong, South Korea

    Seul-Ki Lim

  7. Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, South Korea

    Young-Joon Seo

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  1. Irfan A. Rather
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Contributions

BCK and SKC conceived and designed the experiments. JHL, SKL, and YJS performed the study. IAR, LCL, and RM analyzed the data and wrote the manuscript. GJN and JL provided experimental assistance and services. YHP critically revised for intellectual content.

Corresponding authors

Correspondence to Young-Joon Seo or Yong-Ha Park.

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The study was approved by the Ethical Committee of the Chungnam National University Hospital (IRB No. CNUH 2013-12-020-029) and the clinical trial was registered under Clinical Research Information Service (CRIS) with identifier number KCT0003928.

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Rather, I.A., Kim, BC., Lew, LC. et al. Oral Administration of Live and Dead Cells of Lactobacillus sakei proBio65 Alleviated Atopic Dermatitis in Children and Adolescents: a Randomized, Double-Blind, and Placebo-Controlled Study. Probiotics & Antimicro. Prot. 13, 315–326 (2021). https://doi.org/10.1007/s12602-020-09654-7

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