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In Silico Prediction and In Vitro Assessment of Multifunctional Properties of Postbiotics Obtained From Two Probiotic Bacteria

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Abstract

In this study, a global metabolite profile using Raman spectroscopy analysis was obtained in order to predict, by an in silico prediction of activity spectra for substance approach, the bioactivities of the intracellular content (IC) and cell wall (CW) fractions obtained from Lactobacillus casei CRL 431 and Bacillus coagulans GBI-30 strains. Additionally, multifunctional in vitro bioactivity of IC and CW fractions was also assessed. The metabolite profile revealed a variety of compounds (fatty acids, amino acids, coenzyme, protein, amino sugars), with significant probable activities (Pa > 0.7) as immune-stimulant, anti-inflammatory, neuroprotective, antiproliferative, immunomodulator, and antineoplastic, among others. Moreover, in vitro assays exhibited that both IC and CW fractions presented angiotensin-converting enzyme–inhibitory (> 90%), chelating (> 79%), and antioxidant (ca. 22–57 cellular antioxidant activity units) activities. Our findings based on in silico and in vitro analyses suggest that L. casei CRL 431 and B. coagulans GBI-30 strains appear to be promising sources of postbiotics and may impart health benefits by their multifunctional properties.

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Funding

Aguilar-Toalá was supported by a graduate scholarship from the National Council for Science and Technology (CONACyT) of Mexico. The present work was also funded by Hatch Act formula funds in the College of Agriculture, Purdue University.

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Authors and Affiliations

  1. Laboratorio de Química y Biotecnología de Productos Lácteos, Centro de Investigación en Alimentación y Desarrollo A.C. (CIAD), Carretera a La Victoria km. 0.6, 83304, Hermosillo, Sonora, Mexico

    J. E. Aguilar-Toalá, B. Vallejo-Cordoba, A. F. González-Córdova & A. Hernández-Mendoza

  2. Department of Food Science, Purdue University, 745 Agriculture Mall Dr, West Lafayette, IN, 47907, USA

    J. E. Aguilar-Toalá, F. G. Hall, U. C. Urbizo-Reyes & A. M. Liceaga

  3. UNIDA Tecnológico Nacional de México, Instituto Tecnológico de Veracruz, M.A. de Quevedo 2279, Col. Formando Hogar, 91897, Veracruz, Veracruz, Mexico

    H. S. Garcia

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  1. J. E. Aguilar-Toalá
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  2. F. G. Hall
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A. Hernández-Mendoza and A. M. Liceaga have contributed equally to the direction of this work.

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Correspondence to A. Hernández-Mendoza or A. M. Liceaga.

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Aguilar-Toalá, J.E., Hall, F.G., Urbizo-Reyes, U.C. et al. In Silico Prediction and In Vitro Assessment of Multifunctional Properties of Postbiotics Obtained From Two Probiotic Bacteria. Probiotics & Antimicro. Prot. 12, 608–622 (2020). https://doi.org/10.1007/s12602-019-09568-z

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