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Effect of Potential Probiotic Lactococcus lactis Subsp. lactis on Growth Performance, Intestinal Microbiota, Digestive Enzyme Activities, and Disease Resistance of Litopenaeus vannamei

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Abstract

The aims of this study were to evaluate the effects of Lactococcus lactis subsp. lactis on the growth, intestinal microbiota, digestive enzyme activity, and disease resistance of Litopenaeus vannamei. Diets containing four different concentrations of L. lactis (0 [basal diet], 106, 107, and 108 CFU g−1) were fed to white shrimps L. vannamei (average weight 5.89 ± 0.36 g) for 8 weeks. At the end of the feeding trial, shrimps were immersed in Caspian Seawater (10.8 ppt) contaminated with 106 CFU ml−1 pathogenic V. anguillarum for 2 h. Results revealed that growth rate, survival, and body protein level were increased with dietary supplementation of L. lactis. The activities of digestive enzymes (cellulose, lipase, amylase, and protease) were significantly higher in the groups fed with diets containing 107 or 108 CFU g−1 L. lactis than those in the control. The Lactobacillus and Bacillus counts were higher (P < 0.05) in the intestine of shrimps fed with L. lactis-supplemented diets. In addition, higher level of L. lactis supplementation decreased the Vibrio counts. Moreover, L. vannamei fed diet supplemented with 108 CFU g−1 of L. lactis exhibited significantly the highest hematocyte count and post-challenge survival rate (79.2 %). Collectively, these results suggest that dietary supplementation of L. lactis subsp. lactis at 108 CFU g−1 can promote growth performance, digestive enzyme activity, and disease resistance of L. vannamei.

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Acknowledgments

This study was funded by the Iranian Fisheries Research Organization (Tehran, Iran) and Sari Agricultural Sciences and Natural Resources University.

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Authors and Affiliations

  1. Department of Aquatic Animal Health and Diseases, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran

    Milad Adel

  2. Oceanography Department, Faculty of Science, Alexandria University, Alexandria, Egypt

    Abdel-Fattah M. El-Sayed

  3. Department of Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

    Sakineh Yeganeh

  4. Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

    Maryam Dadar

  5. Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, South Korea

    Sib Sankar Giri

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  1. Milad Adel
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Correspondence to Sib Sankar Giri.

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Adel, M., El-Sayed, AF.M., Yeganeh, S. et al. Effect of Potential Probiotic Lactococcus lactis Subsp. lactis on Growth Performance, Intestinal Microbiota, Digestive Enzyme Activities, and Disease Resistance of Litopenaeus vannamei . Probiotics & Antimicro. Prot. 9, 150–156 (2017). https://doi.org/10.1007/s12602-016-9235-9

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