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Demographic study of imidacloprid-resistant Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) fed on transgenic and commercial potato cultivars

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Abstract

Impacts of transgenic and non-transgenic potato (Solanum tuberosum L.) plants containing more than one insecticidal gene on life table parameters of an imidacloprid-resistant Colorado potato beetle (CPB) colony, Leptinotarsa decemlineata, were studied under laboratory conditions. The life table parameters of the CPB colony on both commercial potato cultivars Agria and Lady Olympia and their transgenic counterparts were studied utilizing two methods: individually reared and group-reared to understand full potential of this destructive pest. All tested CPB larvae and adults on transgenic potato plants died before obtaining any results for the two-sex life table studies. The life table parameters were calculated using non-transgenic plants. The intrinsic rate of increase (r), the finite rate of increase (λ), the net reproductive rate (R0) and the mean generation time (T) were calculated 0.15 day−1, 1.16 day−1, 233.81 eggs/female and 37.43 days on Lady Olympia respectively. The CPBs, reared on cultivar Agria, had significantly lower values of r, λ and R0 except T (0.12 day−1, 1.13 day−1, 120.81 eggs/female and 39.75 days) respectively. The net reproductive rate (R0) (204.31 eggs/female) and the mean generation time (T) 43.86 days of CPB were longer on Lady Olympia than on Agria when reared in groups. All these results indicate that the non-transgenic potato cultivar, Lady Olympia is a more susceptible host for CPB than the potato cultivar Agria and the transgenic potato cultivars exhibit significant toxicity and could be useful in control of imidacloprid-resistant CPB population.

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Acknowledgments

The authors would like to thank the Scientific and Technological Research Council of Turkey (TUBITAK) 2215 Graduate Scholarship Program for International Students (BIDEB) for fellowship and, Niğde Ömer Halisdemir University Scientific Research Projects Coordination (FEB 2017/18-BAGEP) research funding and Niğde Ömer Halisdemir University Agricultural Sciences and Technologies Faculty for providing facilities and equipment for completion of this project. The authors would also like to thank Michael D. Bryan (Plant Industry Program Specialist Pesticide and Plant Pest Management Division) State of Michigan, Department of Agriculture and Rural Development for his diligent proofreading of this manuscript.

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  1. Department of Plant Production and Technologies, Ayhan Shahenk Faculty of Agricultural Sciences and Technologies, Niğde Ömer Halisdemir University, 51240, Niğde, Turkey

    Muhammad Salim & Ayhan Gökçe

  2. Department of Plant Protection, Faculty of Crop Protection Sciences, The University of Agriculture, Peshawar, Pakistan

    Muhammad Salim

  3. Department of Agricultural Genetic Engineering, Ayhan Shahenk Faculty of Agricultural Sciences and Technologies, Niğde Ömer Halisdemir University, 51240, Niğde, Turkey

    Allah Bakhsh

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  1. Muhammad Salim
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Salim, M., Gökçe, A. & Bakhsh, A. Demographic study of imidacloprid-resistant Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) fed on transgenic and commercial potato cultivars. Phytoparasitica 50, 201–221 (2022). https://doi.org/10.1007/s12600-021-00937-5

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