Skip to main content
SpringerLink
Log in

Biochemical resistance to insecticide in Bemisia tabaci field population from Burkina Faso, West Africa

  • Published:
Phytoparasitica Aims and scope Submit manuscript

Abstract

Insecticides are commonly used in greenhouses or fields to control Bemisia tabaci populations leading to rapid evolution of resistance that make treatments inefficient. This resistance can be mediated by molecular and/or biochemical mechanisms. Here, we propose to determine the detoxifying enzymes involved in B. tabaci resistance and to establish the relationship between the enzymatic activity and the values of Lethal Concentrations 50 (LC50). Susceptibility to deltamethrin, acetamiprid and chlorpyrifos-ethyl was assessed, using leaf dip method populations sampled from four (4) sites in Burkina Faso in 2017. Detoxifying enzymes activities including non-specific α-esterases, Para Nitro Phenyl Acetate esterases, oxidases (cytochrome P450) and Glutathione -S-Transferases (GSTs) were measured on single B. tabaci (N = 50) from each test locality and compared with the susceptible strain. In all sites, B. tabaci demonstrated multiple resistance phenotypes. Variation in detoxifying enzyme activities was observed within B. tabaci populations. Elevated levels of non-specific α esterase and GSTs were widespread, suggesting multiple resistance mechanisms occurred within B. tabaci populations from this country.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Abbott, W. S. (1925). The value of the dry substitutes for liquid lime. Journal of Economic Entomology, 18, 265–267.

    Article  CAS  Google Scholar 

  • Alon, M., Benting, J., Lueke, B., Ponge, T., Alon, F., & Morin, S. (2006). Multiple origins of pyrethroid resistance in sympatric biotypes of Bemisia tabaci ( Hemiptera : Aleyrodidae ). Insect Biochemistry and Molecular Biology, 36, 71–79. https://doi.org/10.1016/j.ibmb.2005.10.007.

    Article  PubMed  CAS  Google Scholar 

  • Alon, M., Alon, F., Nauen, R., & Morin, S. (2008). Organophosphates resistance in the B-biotype of Bemisia tabaci ( Hemiptera : Aleyrodidae ) is associated with a point mutation in an ace1 -type acetylcholinesterase and overexpression of carboxylesterase. Insect Biochemistry and Molecular Biology, 38, 940–949. https://doi.org/10.1016/j.ibmb.2008.07.007.

    Article  PubMed  CAS  Google Scholar 

  • Bloch, G. U. Y., & Wool, D. (1994). Methidathion Resistance in the Sweetpotato Whitefly ( Aleyrodidae : Homoptera ) in Israel : Selection , Heritability , and Correlated Changes of Esterase Activity. Insectic. Resist. Resist. Manag., 87, 1147–1156.

    CAS  Google Scholar 

  • Bradford, M. M. (1976). A rapid and sensitive method for the quantitation microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248–254.

    Article  CAS  Google Scholar 

  • Cahill, M., Byrne, F. J., Denholm, I., Devonshire, A. I., & Gorman, K. J. (1995). Pyrethroid and organophosphate resistance in the tobacco whitefly Bemisia tabaci (Gennadius) (Homoptera Aleyrodidae). Bulletin of Entomological Research, 85, 181–187. https://doi.org/10.1017/S0007485300034258.

    Article  CAS  Google Scholar 

  • Carletto, J., Martin, T., Vanlerberghe-Masuttia, F., & Thierry, B. (2009). Insecticide resistance traits differ among and within host races in Aphis gossypii J er. Pest Management Science. https://doi.org/10.1002/ps.1874.

    Article  CAS  Google Scholar 

  • Dângelo, R. A. C., Campos, M. R., Silva, P. S., & Guedes, R. N. C. (2018). Insecticide resistance and control failure likelihood of the whitefly Bemisia tabaci ( MEAM1 ; B biotype ): a Neotropical. The Annals of Applied Biology, 172, 88–99. https://doi.org/10.1111/aab.12404.

    Article  CAS  Google Scholar 

  • De Barro, P. J., Liu, S., Boykin, L. M., & Dinsdale, A. B. (2011). Bemisia tabaci : A statement of species status. Annual Review of Entomology, 56, 1–19. https://doi.org/10.1146/annurev-ento-112408-085504.

    Article  PubMed  CAS  Google Scholar 

  • Denholm, I., Cahill, M., Dennehy, T. J., Horowitz, A. R., Trans, P., Lond, R. S., Denholm, I., Cahill, M., Dennehy, T. J., & Horowitz, A. R. (1998). Challenges with managing insecticide resistance in agricultural pests , exemplisfied by the whitefly Bemisia tabaci Challenges with managing insecticide resistance in agricultural pests , exempli ® ed by the white ¯ y Bemisia tabaci. R. Soc., 1757–1767. https://doi.org/10.1098/rstb.1998.0328.

    Article  CAS  Google Scholar 

  • Dennehy, T. J., Degain, B. A., Harpold, V. S., Morin, S., Fabrick, J. A., Nichols, R. L., Brown, J. K., Byrne, J., Li, X., Dennehy, T. J., Degain, B. A., Harpold, V. S., Zaborac, M., Morin, S., Fabrick, J. A., Nichols, R. L., Brown, J. K., Byrne, F. J., & Li, X. (2010). Extraordinary resistance to insecticides reveals exotic Q biotype of Bemisia tabaci in the New World. Insectic. Resist. Resist. Manag., 103, 2174–2186. https://doi.org/10.1603/EC10239.

    Article  CAS  Google Scholar 

  • Drabo, F. S., Olivier, G., Bassole, I. H. N., Ne, R. C., & Lyon, D. (2017). Susceptibility of MED-Q1 and MED-Q3 biotypes of Bemisia tabaci ( Hemiptera : Aleyrodidae ) populations to essential and seed oils. Journal of Economic Entomology, 1–8. https://doi.org/10.1093/jee/tox100.

    Article  CAS  Google Scholar 

  • Gelman, D. B., Gerling, D., & Blackburn, M. A. (2005). Host-parasitoid interactions relating to penetration of the whitefly , Bemisia tabaci , by the parasitoid wasp , Eretmocerus mundus. J. Insect Sci, 1–14.

  • Gnankine, O., Mouton, L., Henri, N. E., Martin, T., Raz, G. T. E. R., & Houndete, T. (2013c). Distribution of Bemisia tabaci ( Homoptera : Aleyrodidae ) biotypes and their associated symbiotic bacteria on host plants in West Africa. Insect Conserv. Divers., 6, 411–421. https://doi.org/10.1111/j.1752-4598.2012.00206.x.

    Article  Google Scholar 

  • Gnankiné, O., Bassolé, I. H. N., Chandre, F., Glitho, I., Akogbeto, M., Dabiré, R. K., & Martin, T. (2013a). Acta Tropica insecticide resistance in Bemisia tabaci Gennadius ( Homoptera : Aleyrodidae ) and Anopheles gambiae Giles ( Diptera : Culicidae ) could compromise the sustainability of malaria vector control strategies in West Africa. Acta Tropica, 128, 7–17. https://doi.org/10.1016/j.actatropica.2013.06.004.

    Article  PubMed  CAS  Google Scholar 

  • Gnankiné, O., Mouton, L., Savadogo, A., Martin, T., Sanon, A., Dabire, R. K., Vavre, F., & Fleury, F. (2013b). Biotype status and resistance to neonicotinoids and carbosulfan in Bemisia tabaci ( Hemiptera : Aleyrodidae ) in Burkina Faso. West Africa. Int. J. Pest Manag., 59, 95–102.

    Article  CAS  Google Scholar 

  • Gnankiné, O., Hema, O., Namountougou, M., Mouton, L., & Vavre, F. (2018). Impact of pest management practices on the frequency of insecticide resistance alleles in Bemisia tabaci ( Hemiptera : Aleyrodidae ) populations in three countries of West Africa. Crop Protection, 104, 86–91. https://doi.org/10.1016/j.cropro.2017.10.020.

    Article  Google Scholar 

  • Guedes, R. N. C. (2016). Insecticide resistance , control failure likelihood and the First Law of Geography. Pest Biochem Physiol, 73, 479–484. https://doi.org/10.1002/ps.4452.

    Article  CAS  Google Scholar 

  • Guo, L., Xie, W., Wang, S., Wu, Q., Li, R., Yang, N., Pan, H., & Zhang, Y. (2014). Detoxification enzymes of Bemisia tabaci B and Q : Biochemical characteristics and gene expression profiles. Pest Management Science, 70, 1558–1594. https://doi.org/10.1002/ps.3751.

    Article  CAS  Google Scholar 

  • He, C., Xie, W., Yang, X., & Wang, S. (2018). Identification of glutathione S-transferases in Bemisia tabaci ( Hemiptera : Aleyrodidae ) and evidence that GSTd7 helps explain the difference in insecticide susceptibility between B . tabaci Middle East-Minor Asia 1 and Mediterranean. R. Entomol. Soc., 27, 22–35. https://doi.org/10.1111/imb.12337.

    Article  CAS  Google Scholar 

  • Henri, H., Terraz, G., Gnankiné, O., Fleury, F., Mouton, L. (2013) Molecular characterization of genetic diversity within the Africa/Middle East/Asia Minor and Sub-Saharan African groups of the species complex. International Journal of Pest Management 59 (4):329-338

    Article  CAS  Google Scholar 

  • Hemingway, J. (2000). The molecular basis of two contrasting metabolic mechanisms of insecticide resistance. Insect Biochemistry and Molecular Biology, 30, 1009–1015.

    Article  CAS  Google Scholar 

  • Hemingway, J., Hawkes, N. J., Mccarroll, L., & Ranson, H. (2004). The molecular basis of insecticide resistance in mosquitoes. Insect Biochemistry and Molecular Biology, 34, 653–665. https://doi.org/10.1016/j.ibmb.2004.03.018.

    Article  PubMed  CAS  Google Scholar 

  • Horowitz, A.R., Kontsedalov, S., Khasdan, V., Ishaaya, I., 2005. Biotypes B and Q of Bemisia tabaci and their relevance to Neonicotinoid and Pyriproxyfen resistance 225, 216–225. https://doi.org/10.1002/arch.20044.

    Article  CAS  Google Scholar 

  • Houndété, T. A., Fournier, D., Ketoh, G. K., Glitho, I. A., Nauen, R., & Martin, T. (2010a). Biochemical determination of acetylcholinesterase genotypes conferring resistance to the organophosphate insecticide chlorpyriphos in field populations of Bemisia tabaci from Benin , West Africa. Pest Biochem Physiol, 98, 115–120. https://doi.org/10.1016/j.pestbp.2010.05.009.

    Article  CAS  Google Scholar 

  • Houndété, T., Thierry, B., Hema, S. A., Glitho, I. A., & Martin, T. (2010b). Insecticide resistance in field populations of Bemisia tabaci ( Hemiptera : Aleyrodidae ) in West Africa. Pest Management Science, 66, 1181–1185. https://doi.org/10.1002/ps.2008.

    Article  PubMed  CAS  Google Scholar 

  • Karunker, I., Benting, J., Lueke, B., Ponge, T., Nauen, R., Roditakis, E., Vontas, J., Gorman, K., Denholm, I., & Morin, S. (2008). Over-expression of cytochrome P450 CYP6CM1 is associated with high resistance to imidacloprid in the B and Q biotypes of Bemisia tabaci ( Hemiptera : Aleyrodidae ). Insect Biochemistry and Molecular Biology, 38, 634–644. https://doi.org/10.1016/j.ibmb.2008.03.008.

    Article  PubMed  CAS  Google Scholar 

  • Li, X., Schuler, M. A., & Berenbaum, M. R. (2007). Molecular mechanisms of metabolic resistance to synthetic and natural Xenobiotics. Annals of the Entomological Society of America, 52, 231–255. https://doi.org/10.1146/annurev.ento.51.110104.151104.

    Article  CAS  Google Scholar 

  • Morin, S., Williamson, M. S., Goodson, S. J., Brown, J. K., & Tabashnik, B. E. (2002). Mutations in the Bemisia tabaci para sodium channel gene associated with resistance to a pyrethroid plus organophosphate mixture. Insect Biochemistry and Molecular Biology, 32, 1781–1791.

    Article  CAS  Google Scholar 

  • Mouton, L., Gnankiné, O., Henri, H., Terraz, G., Ketoh, G., Martin, T., & Vavre, F. (2014). Detection of genetically isolated entities within the Mediterranean species of Bemisia tabaci : New insights into the systematics of this worldwide pest. Pest Management Science, 71, 452–458. https://doi.org/10.1002/ps.3834.

    Article  PubMed  CAS  Google Scholar 

  • Nauen, R., Vontas, J., & Katharina, W. (2013). Pymetrozine is hydroxylated by CYP6CM1 , a cytochrome P450 conferring neonicotinoid resistance in Bemisia tabaci. Pest Management Science, 69, 457–461. https://doi.org/10.1002/ps.3460.

    Article  PubMed  CAS  Google Scholar 

  • Otoidobiga, L. C., Vincent, C., & Stewart, R. K. (2003). Field efficacy and baseline toxicities of Pyriproxifen, Acetamiprid, and Diafenthiuron against Bemisia tabaci Gennadius (Homoptera: Aleyrodidae) in Burkina Faso (West Africa). J. Environ. Sci. Heal., B38, 757–769. https://doi.org/10.1081/PFC-120025559.

    Article  CAS  Google Scholar 

  • Panini, M., Tozzi, F., Zimmer, C. T., Bass, C., Field, L., Borzatta, V., & Moores, G. (2017). Biochemical evaluation of interactions between synergistic molecules and phase I enzymes involved in insecticide resistance in B- and Q-type Bemisia tabaci ( Hemiptera : Aleyrodidae ). Pest Biochem Physiol, 73, 1873–1882. https://doi.org/10.1002/ps.4553.

    Article  CAS  Google Scholar 

  • Peng, Z., Zheng, H., Xie, W., Wang, S., Wu, Q., & Zhang, Y. (2017). Field resistance monitoring of the immature stages of the whitefly Bemisia tabaci to spirotetramat in China. Crop Protection, 98, 243–247. https://doi.org/10.1016/j.cropro.2017.04.009.

    Article  CAS  Google Scholar 

  • Perring, T. M. (2001). The Bemisia tabaci species complex. Crop Protection, 20, 725–737.

    Article  Google Scholar 

  • Roditakis, E., Tsagkarakou, A., Vontas, J., 2006. IdentiWcation of mutations in the para sodium channel of Bemisia tabaci from Crete , associated with resistance to pyrethroids 85, 161–166. https://doi.org/10.1016/j.pestbp.2005.11.007.

    Article  CAS  Google Scholar 

  • Roditakis, E., Grispou, M., Morou, E., Kristoffersen, J. B., Roditakis, N., Nauen, R., & Tsagkarakou, A. (2009). Current status of insecticide resistance in Q biotype Bemisia tabaci populations from Crete. Pest Management Science, 65, 313–322. https://doi.org/10.1002/ps.1690.

    Article  PubMed  CAS  Google Scholar 

  • Romba, R., Gnankine, O., Fogné, S., Fidèle, D., Henri, H., Mouton, L., & Vavre, F. (2018). Abundance of Bemisia tabaci Gennadius ( Hemiptera : Aleyrodidae ) and its parasitoids on vegetables and cassava plants in Burkina Faso ( West Africa ). Ecology and Evolution, 1–13. https://doi.org/10.1002/ece3.4078.

    Article  Google Scholar 

  • Roush, R.T., and, Daly, J.C., 1990. The role of population genetics in Résistance research and management, in: Richard T. Roush and Bruce E. Tabashnick (Ed.), pesticide resistance in arthropods. New York and London, pp. 97–152. https://doi.org/10.1007/978-1-4684-6429-0.

    Google Scholar 

  • Rowland, M., Hackete, B., & Stribley, M. (1991). Evaluation of insecticides in field control simulators and standard laboratory bioassays against resistant and susceptible Bemisia tabaci (Homoptera Aleyrodidae) from Sudan. Bulletin of Entomological Research, 81, 189–199.

    Article  CAS  Google Scholar 

  • Scharf, M. E., Neal, J. J., & Bennett, G. W. (1998). Changes of Insecticide Resistance Levels and Detoxication Enzymes Following Insecticide Selection in the German Cockroach , Blattella germanica ( L .). Pesticide Biochemistry and Physiology, 79, 67–79.

    Google Scholar 

  • Schchukin, A., & Wool, D. (1994). Pyrethroid resistance and esterase activity in selected laboratory populations of sweetpotato whiteflis Bemisia tabaci (Homoptera: Aleyrodidae). European Journal of Entomology, 91, 285–295.

    Google Scholar 

  • Simmons, A. M., Harrison, H. F., & Ling, K. (2008). Forty-nine new host plant species for Bemisia tabaci ( Hemiptera : Aleyrodidae ). Entomological Science, 11, 385–390. https://doi.org/10.1111/j.1479-8298.2008.00288.x.

    Article  Google Scholar 

  • Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H., Flook, P. (1994) Evolution, Weighting, and Phylogenetic Utility of Mitochondrial Gene Sequences and a Compilation of Conserved Polymerase Chain Reaction Primers. Annals of the Entomological Society of America 87 (6):651-701

    Article  CAS  Google Scholar 

  • Tsagkarakou, A., Nikou, D., Roditakis, E., Sharvit, M., Morin, S., & Vontas, J. (2009). Molecular diagnostics for detecting pyrethroid and organophosphate resistance mutations in the Q biotype of the whitefly Bemisia tabaci ( Hemiptera : Aleyrodidae ). Pesticide Biochemistry and Physiology, 94, 49–54. https://doi.org/10.1016/j.pestbp.2009.03.002.

    Article  CAS  Google Scholar 

  • Vontas, J. G., Small, G. J., & Hemingway, J. (2001). resistance in Nilaparvata lugens. Biochem. Soc., 72, 65–72.

    Article  Google Scholar 

  • Wang, R., Fang, Y., Mu, C., Qu, C., Li, F., Wang, Z., & Luo, C. (2017a). Baseline susceptibility and cross-resistance of cycloxaprid , a novel cis-nitromethylene neonicotinoid insecticide , in Bemisia tabaci MED from China. Crop Prot, 1–5. https://doi.org/10.1016/j.cropro.2017.02.012.

    Article  CAS  Google Scholar 

  • Wang, S., Zhang, Y., Yang, X., Xie, W., & Wu, Q. (2017b). Insecticide resistance and resistance management resistance monitoring for eight insecticides on the Sweetpotato whitefly ( Hemiptera : Aleyrodidae ) in China. Journal of Economic Entomology, 1–7. https://doi.org/10.1093/jee/tox040.

    Article  Google Scholar 

  • Yuan, L., Wang, S., Zhou, J., Du, Y., Zhang, Y., & Wang, J. (2012). Status of insecticide resistance and associated mutations in Q-biotype of whitefly , Bemisia tabaci , from eastern China. Crop Protection, 31, 67–71. https://doi.org/10.1016/j.cropro.2011.09.017.

    Article  CAS  Google Scholar 

  • Zhang, B., Kong, F., & Cui, R. (2016). Gene expression of detoxification enzymes in insecticide-resistant and insecticide-susceptible Bemisia tabaci strains after diafenthiuron exposure. The Journal of Agricultural Science, 154, 742–753. https://doi.org/10.1017/S0021859615001409.

    Article  CAS  Google Scholar 

  • Zheng, H., Xie, W., Wang, S., Wu, Q., Zhou, X., & Zhang, Y. (2017). Dynamic monitoring ( B versus Q ) and further resistance status of Q- type Bemisia tabaci in China. Crop Protection, 94, 115–122. https://doi.org/10.1016/j.cropro.2016.11.035.

    Article  Google Scholar 

Download references

Acknowledgments

This study was funded by TWAS (The World Academic of Sciences) Project of Université of Ouaga I Pr Joseph Ki-ZERBO. Drabo Fogné Samuel is the recipient of a PhD studentship of University of Ouaga I Pr Joseph Ki-Zerbo.

Author information

Authors and Affiliations

  1. Laboratoire d’Entomologie Appliquée, Université de Ouaga I Pr Joseph Ki-ZERBO, Ouagadougou, Burkina Faso

    Drabo Samuel Fogné, Romba Rahim, Balboné Mahamoudou & Gnankiné Olivier

  2. Laboratoire de Biochimie & Chimie Appliquées, UFR/SVT, Université de Ouaga I Pr Joseph Ki-ZERBO, Ouagadougou, Burkina Faso

    Coulibaly Y. Ahmed

Authors
  1. Drabo Samuel Fogné
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Coulibaly Y. Ahmed
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Romba Rahim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Balboné Mahamoudou
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Gnankiné Olivier
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Drabo Samuel Fogné.

Ethics declarations

Conflict of interest

The author declares that he has no conflict interest.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Fogné, D.S., Ahmed, C.Y., Rahim, R. et al. Biochemical resistance to insecticide in Bemisia tabaci field population from Burkina Faso, West Africa. Phytoparasitica 47, 671–681 (2019). https://doi.org/10.1007/s12600-019-00765-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12600-019-00765-8

Keywords

Search

Navigation