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Detection of Potato virus Y in industrial quantities of seed potatoes by TaqMan Real Time PCR

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Abstract

Potato (Solanum tuberosum) is the largest crop in Israel. Production is based on the import of seed tubers from Europe for the spring crop. Imported tubers are generally free from virus infection. The most important virus infecting potato is Potato virus Y (PVY), which may cause severe damage to marketable yields. In Israel, tubers from the spring harvest are stored over the summer for planting in the autumn. It is important to be able to determine the infection rate of seed tuber lots from the spring harvest prior to storage. Commonly, infection is measured by sprouting tubers and measuring virus titre in the leaves using ELISA (the “Growing-On test”), which takes at least 6 weeks to give results. There is a need for a faster method to produce results, such as Taqman Real Time PCR (qPCR), for direct analysis of viral infection in tubers at harvest. To use qPCR as a diagnostic tool, it is necessary to demonstrate that both techniques give comparable results on batches of field-grown tubers. Such a comparison was performed on potential seed tuber lots of 14 different cultivars over three Israeli spring harvests (2013–2015). The agreement between the results of the two techniques was not of high statistical significance. However, the qPCR technique can distinguish well, by binary classification, between tuber lots with a low PVY infection rate (<5% by Growing On test; suitable for seed) and those unsuitable for seed (≥5% by Growing On test). Therefore, qPCR is an appropriate technique for determination of the PVY infection rate of seed tuber lots in Israel.

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Acknowledgements

Contribution from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel, No. 560/16. The authors thank Dr. Christophe Lacomme for critical comments on the ms, Prof. Dani Shtienberg for the curve-fitting and statistical assistance and the Israeli Plant Council (grant nos. 132163012, 132168113, 132181014 and 132183515) and the Chief Scientist of the Israeli Ministry of Agriculture (grant no. 132171614) for partial support.

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Author notes

  1. Liat Avrahami-Moyal and Yehudit Tam equally contributed to this work.

Authors and Affiliations

  1. Department of Plant Pathology and Weed Science, ARO Volcani Center, HaMaccabim Road, P.O.B 15159, 7528809, Rishon LeTsiyon, Israel

    Liat Avrahami-Moyal, Yehudit Tam, Marina Brumin, Shanmugam Prakash, Diana Leibman, Malenia Pearlsman, Menachem Bornstein, Noa Sela, Amit Gal-On & Victor Gaba

  2. Robert H. Smith Faculty of Agriculture, Hebrew University, Rehovot, Israel

    Liat Avrahami-Moyal

  3. Plant Protection Inspection Service, Ministry of Agriculture, Bet Dagan, Israel

    Mouhammad Zeidan

  4. Agricultural Advisory Service, Ministry of Agriculture, Bet Dagan, Israel

    Zion Dar

  5. Maon Enterprises Ltd., MP Negev, 85465, Israel

    Uri Zig

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  1. Liat Avrahami-Moyal
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  2. Yehudit Tam
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  13. Victor Gaba
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Correspondence to Victor Gaba.

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Avrahami-Moyal, L., Tam, Y., Brumin, M. et al. Detection of Potato virus Y in industrial quantities of seed potatoes by TaqMan Real Time PCR. Phytoparasitica 45, 591–598 (2017). https://doi.org/10.1007/s12600-017-0612-z

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  • DOI: https://doi.org/10.1007/s12600-017-0612-z

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