Skip to main content

Advertisement

SpringerLink
Log in

Molecular characterization and phylogenetic analysis of a Greek lentil isolate of Pea seed-borne mosaic virus

  • Published:
Phytoparasitica Aims and scope Submit manuscript

Abstract

Legume crop cultivation is widespread in Greece and contributes significantly to the agricultural economy of the country. In contrast to pea, Pea seed-borne mosaic virus (PSbMV) detection and pathotype characterization in lentil is limited. The aim of this study was the molecular characterization of the first reported lentil isolate of PSbMV in Greece. Specific primers against three genes of PSbMV, namely the coat protein (CP) and the P1 and HC-Pro proteases have been designed and tested in RT-PCR detection and quantitation of the virus in lentil. Phylogenetic and recombination analysis of the Greek CP and HC-Pro amplicons was performed with the aim of detecting sequence variability indicative of pathotype classification. Moreover, the identified point mutations of the Greek HC-Pro sequence were further exploited in RT-RFLP protocols that together with the phylogenetic analysis lead to the classification of the Greek PSbMV isolate into pathogroup 1.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Literature

  • Alconero, R., Provvidenti, R., & Gonsalves, D. (1986). Three Pea seed-borne mosaic virus pathotypes from pea and lentil germ plasm. Plant Disease, 70, 783–786.

    Article  Google Scholar 

  • Aleman-Verdaguer, M. E., Goudou-Urbino, C., Dubern, J., Beachy, R. N., & Fauquet, C. (1997). Analysis of the sequence diversity of the P1, HC, P3, NIb and CP genomic regions of several yam mosaic potyvirus isolates: implications for the intraspecies molecular diversity of potyviruses. Journal of General Virology, 78, 1253–1264.

    Article  PubMed  Google Scholar 

  • Ali, A., & Randles, J. W. (1997). Early season survey of pea viruses in Pakistan and the detection of two new pathotypes of pea seedborne mosaic potyvirus. Plant Disease, 81, 343–347.

    Article  Google Scholar 

  • Ali, A., & Randles, J. W. (2001). Genomic heterogeneity in Pea seed-borne mosaic virus isolates from Pakistan, the centre of diversity of the host species, Pisum sativum. Archives of Virology, 146, 1855–1870.

    Article  CAS  PubMed  Google Scholar 

  • Andersen, K., & Johansen, I. E. (1998). A single conserved amino acid in the coat protein gene of pea seed-borne mosaic potyvirus modulates the ability of the virus to move systemically in Chenopodium quinoa. Virology, 241, 304–311.

    Article  CAS  PubMed  Google Scholar 

  • Andrejeva, J., Puurand, U., Merits, A., Rabenstein, F., Järvekülg, L., & Valkonen, J. P. (1999). Potyvirus helper component-proteinase and coat protein (CP) have coordinated functions in virus–host interactions and the same CP motif affects virus transmission and accumulation. Journal of General Virology, 80, 1133–1139.

    Article  CAS  PubMed  Google Scholar 

  • Blanc, S., Ammar, E. D., Garcia-Lampasona, S., Dolja, V. V., Llave, C., Baker, J., et al. (1998). Mutations in the potyvirus helper component protein: effects on interactions with virions and aphid stylets. Journal of General Virology, 79, 3119–3122.

    Article  CAS  PubMed  Google Scholar 

  • Chatzivassiliou, Ε. Κ., Boubourakas, H., Winter, S., Lesemann, D. E., Avgelis, A. D., & Katis, N. I. (2002). Viruses infecting pea (Pisum sativum L.) crops in Greece. Phytopathologia Mediterranea, 45, 82.

    Google Scholar 

  • Chatzivassiliou, Ε. Κ., Kargiotidou, A., Tokatlidis, I., Kumari, S. G., & Makkouk, K. M. (2010). The incidence of viruses in lentil crops and breeding perspectives in Greece. 15th Hellenic Phytopathological Congress. Corfu, Greece. Abstracts p. 105 (in Greek).

  • Chung, B. Y., Miller, W. A., Atkins, J. F., & Firth, A. E. (2008). An overlapping essential gene in the Potyviridae. PNAS, 105, 5897–5902.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Coutts, B. A., Prince, R. T., & Jones, R. A. C. (2009). Quantifying effects of seedborne inoculum on virus spread, yield losses, and seed infection in the Pea seed-borne mosaic virus–field pea pathosystem. Phytopathology, 99, 1156–1167.

    Article  CAS  PubMed  Google Scholar 

  • Goodell, J. J., & Hampton, R. O. (1984). Ecological characteristics of the lentil strain of pea seedborne mosaic virus. Plant Disease, 68, 148–150.

    Article  Google Scholar 

  • Hampton, R. O. (1982). Incidence of the lentil strain of Pea seed-borne mosaic virus as a contaminant of Lens culinaris germ plasm. Phytopathology, 72, 695–698.

    Article  Google Scholar 

  • Hampton, R. O., Mink, G. I., Bos, L., Inouye, T., Musil, M., & Hagedorn, D. J. (1981). Host differentiation and serological homology of Pea seed-borne mosaic virus isolates. Netherlands Journal of Plant Pathology, 87, 1–10.

    Article  Google Scholar 

  • Hampton, R. O., Kraft, J. M., & Muehlbauer, F. J. (1993). Minimizing the threat of seedborne pathogens in crop germplasm: elimination of Pea seedborne mosaic virus from the USDA-ARS germ plasm collection of Pisum sativum. Plant Disease, 77, 220–224.

    Article  Google Scholar 

  • Hjulsager, C. K., Lund, O. S., & Johansen, I. E. (2002). A new pathotype of Pea seed-borne mosaic virus explained by properties of the P3-6k1- and viral genome-linked protein (VPg)-coding regions. Molecular Plant-Microbe Interactions, 15, 169–171.

    Article  CAS  PubMed  Google Scholar 

  • Johansen, E., Rasmussen, F., Heide, M., & Borkhardt, B. (1991). The complete nucleotide sequence of Pea seed-borne mosaic virus RNA. Journal of General Virology, 72, 2625–2632.

    Article  CAS  PubMed  Google Scholar 

  • Johansen, I. E., Keller, K. E., Dougherty, W. G., & Hampton, R. O. (1996). Biological and molecular properties of a pathotype P-1 and a pathotype P-4 isolate of Pea seed-borne mosaic virus. Journal of General Virology, 77, 1329–1333.

    Article  CAS  PubMed  Google Scholar 

  • Jones, R. A. C. (2004). Using epidemiological information to develop effective integrated virus disease management strategies. Virus Research, 100, 5–30.

    Article  CAS  PubMed  Google Scholar 

  • Kargiotidou, A., Chatzivassiliou, E. K., Tzantarmas, C., & Tokatlidis, I. S. (2015). Seed propagation at low density facilitates the selection of healthy plants to produce seeds with a reduced virus load in a lentil landrace. Seed Science and Technology, 43, 31–39.

    Article  Google Scholar 

  • Kasschau, K. D., & Carrington, J. C. (1998). A counterdefensive strategy of plant viruses: suppression of posttranscriptional gene silencing. Cell, 95, 461–470.

    Article  CAS  PubMed  Google Scholar 

  • Kavallieratos, N., Tomanović, Ž., Sarlis, P. G., Vayias, B. J., Žikić, V., & Emmanouel, N. E. (2007). Aphids (Hemiptera: Aphidoidea) on cultivated and self-sown plants in Greece. Biologia, 62, 335–344.

    Article  Google Scholar 

  • Khetarpal, R. K., & Maury, Y. (1987). Pea seed-borne mosaic virus: a review. Agronomie, 7, 215–224.

    Article  Google Scholar 

  • Khetarpal, R. K., Maury, Y., Cousin, R., Burghofer, A., & Varma, A. (1990). Study on resistance of pea to Pea seed-borne mosaic virus and new pathotypes. Annals of Applied Biology, 116, 297–304.

    Article  Google Scholar 

  • Kohnen, P. D., Johansen, I. E., & Hampton, R. O. (1995). Characterization and molecular detection of the P-4 pathotype of Pea seed-borne mosaic potyvirus. Phytopathology, 85, 789–793.

    Article  CAS  Google Scholar 

  • Lee, K. C., & Wong, S. M. (1998). Variability of P1 protein of Zucchini yellow mosaic virus for strain differentiation and phylogenetic analysis with other potyviruses. DNA Sequence, 9, 275–293.

    CAS  PubMed  Google Scholar 

  • Makkouk, K. M., Kumari, S. G., & Bos, L. (1993). Pea seed-borne mosaic virus: occurrence in faba bean (Vicia faba L.) and lentil (Lens culinaris Med.) in West Asia and North Africa, and further information on host range, purification, serology, and transmission characteristics. Netherlands Journal of Plant Pathology, 99, 115–124.

    Article  Google Scholar 

  • Makkouk, K. M., Kumari, S. G., van Leur, J. A. G., & Jones, R. A. C. (2014). Control of plant virus diseases in cool-season grain legume crops. Advances in Virus Research, 90, 207–254.

    Article  PubMed  Google Scholar 

  • Musil, M. (1966). Uber das Vorkommen des Virus des Blattrollens der Erbse in der Slowakei (Vorlaufige Mitteilung). Biologia (Bratislava), 21, 133–138.

    Google Scholar 

  • Olmos, A., Bertolini, E., Gil, M., & Cambra, M. (2005). Real-time assay for quantitative detection of non-persistently transmitted Plum pox virus RNA targets in single aphids. Journal of Virological Methods, 128, 151–155.

    Article  CAS  PubMed  Google Scholar 

  • Resuehr, D., & Spiess, A.-N. (2003). A real-time polymerase chain reaction-based evaluation of cDNA synthesis priming methods. Analytical Biochemistry, 322, 287–291.

    Article  CAS  PubMed  Google Scholar 

  • Revers, F., & García, J. A. (2015). Molecular biology of potyviruses. Advances in Virus Research, 92, 101–199.

    Article  PubMed  Google Scholar 

  • Roudet-Tavert, G., German-Retana, S., Delaunay, T., Delecolle, B., Candresse, T., & Le Gall, O. (2002). Interaction between potyvirus helper component-proteinase and capsid protein in infected plants. Journal of General Virology, 83, 1765–1770.

    Article  CAS  PubMed  Google Scholar 

  • Shiboleth, Y. M., Haronsky, E., Leibman, D., Arazi, T., Wassenegger, M., Whitham, S. A., Gaba, V., & Gal-On, A. (2007). The conserved FRNK box in HC-Pro, a plant viral suppressor of gene silencing, is required for small RNA binding and mediates symptom development. Journal of Virology, 81, 13135–13148.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Soitamo, A. J., Jada, B., & Lehto, K. (2011). HC-Pro silencing suppressor significantly alters the gene expression profile in tobacco leaves and flowers. BMC Plant Biology. doi:10.1186/1471-2229-11-68.

    PubMed Central  PubMed  Google Scholar 

  • Syller, J. (2005). The roles and mechanisms of helper component proteins encoded by potyviruses and caulimoviruses. Physiological and Molecular Plant Pathology, 67, 119–130.

    Article  CAS  Google Scholar 

  • Torok, V. A., & Randles, J. W. (2007). Discriminating between isolates of PSbMV using nucleotide sequence polymorphisms in the HC-Pro coding region. Plant Disease, 91, 490–496.

    Article  CAS  Google Scholar 

  • Tsitsipis, J. A., Kati, N. I., Margaritopoulos, J. T., Lykouressis, D. P., Avgelis, A. D., Gargalianou, I., Zarpas, K. D., Perdikis, D. C., & Papapanayotou, A. (2007). A contribution to the aphid fauna of Greece. Bulletin of Instectology, 60, 31–38.

    Google Scholar 

  • van Leur, J. A. G., Freeman, A. E., Aftab, M., Spackman, M., Redden, B., & Materne, M. (2013). Identification of seed-borne Pea seed-borne mosaic virus in lentil (Lens culinaris) germplasm and strategies to avoid its introduction in commercial Australian lentil fields. Australasian Plant Disease Notes. doi:10.1007/s13314-013-0099-5.

    Google Scholar 

  • Vijayapalani, P., Maeshima, M., Nagasaki-Takekuchi, N., & Miller, W. A. (2012). Interaction of the trans-frame potyvirus protein P3N-PIPO with host protein PCaP1 facilitates potyvirus movement. PLoS Pathogens, 8, e1002639.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Wen, R. H., & Hajimorad, M. R. (2010). Mutational analysis of the putative pipo of soybean mosaic virus suggests disruption of PIPO protein impedes movement. Virology, 400, 1–7.

    Article  CAS  PubMed  Google Scholar 

  • Wylie, S. J., Coutts, B. A., & Jones, R. A. C. (2011). Genetic variability of the coat protein sequence of Pea seed-borne mosaic virus isolates and the current relationship between phylogenetic placement and resistance groups. Archives of Virology, 156, 1287–1290.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This research has been co-financed by the European Union (European Social Fund – ESF) and Greek national funds through the Operational Program "Education and Lifelong Learning" of the National Strategic Reference Framework (NSRF) ‐ Research Funding Program: THALES (Investing in knowledge society through the European Social Fund).

Author information

Authors and Affiliations

  1. Plant Pathology Laboratory, Department of Crop Science, Agricultural University of Athens, Iera Odos 75, 11855, Athens, Greece

    Antonis Giakountis, Aikaterini Skoufa, Epameinondas I. Paplomatas & Elisavet K. Chatzivassiliou

  2. Department of Agricultural Development, Democritus University of Thrace, Pantazidou 193, 68200, Orestiada, Greece

    Ioannis S. Tokatlidis

Authors
  1. Antonis Giakountis
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Aikaterini Skoufa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Epameinondas I. Paplomatas
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ioannis S. Tokatlidis
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Elisavet K. Chatzivassiliou
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Elisavet K. Chatzivassiliou.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Giakountis, A., Skoufa, A., Paplomatas, E.I. et al. Molecular characterization and phylogenetic analysis of a Greek lentil isolate of Pea seed-borne mosaic virus . Phytoparasitica 43, 615–628 (2015). https://doi.org/10.1007/s12600-015-0495-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12600-015-0495-9

Keywords

Search

Navigation