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Protective effect of oil extracted from Neophocaena asiaeorientalis against hydgrogen peroxide-induced oxidative stress in HepG2 cells

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  • Food Science and Technology
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Abstract

Although animal fat has been reported to be an effective preventative agent for cardiovascular disease, pancreatitis and diabetes, its protective effects against hepatotoxicity have not been thoroughly investigated. Therefore, in this study, the anti-hepatotoxic activity of oil extracted from the forehead of Neophocaena asiaeorientalis (OFNA) was investigated. Changes in cell viability, cell morphology, cell cycle, and apoptotic protein expression were measured in HepG2 cells co-treated with hydrogen peroxide (H2O2) and OFNA. OFNA contained 19.3 g/100 g docosahexaenoic acid, 8.2 g/100 g eicosapentaenoic acid, and 19.6 g/100 g palmitoleic acid. Cell viability did not differ significantly between vehicle-treated and 0.1–1% OFNA-treated cells. The minimum concentration of H2O2 required to cause the death of HepG2 cells was 1 mM. Cell viability was higher in the group treated with H2O2 plus OFNA (0.1, 0.5, 0.7 and 1%) than in the group treated with H2O2 alone, and OFNA protected against H2O2-induced apoptosis in a dose-dependent manner. The ratio of Bax/Bcl-2 was reduced in response to treatment with the OFNA combination compared to H2O2 alone. Interestingly, 2′,7′-dichlorodihydrofluorescein diacetate staining showed a reduction of oxidative stress induced by H2O2 after pre-treatment with OFNA. Taken together, these results suggest that OFNA protects cells against H2O2-induced death, and suggest that it has potential for use as a preventive agent for various types of hepatic injury caused by chemical substances.

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References

  • Castillo M, Amalik F, Linares A, Garcia-Peregrin E (2000) Fish oil reduces cholesterol and arachidonic acid levels in plasma and lipoproteins from hypercholesterolemic chicks. Mol Cell Biochem 210:121–130

    Article  CAS  PubMed  Google Scholar 

  • Chun B-S (2010) Phospholipids isolation from squid viscera residues after supercritical carbon dioxide extraction. Korean Chem Eng Res 48:741–746

    Google Scholar 

  • Cobbina E, Akhlaghi F (2017) Non-alcoholic fatty liver disease (NAFLD)—pathogenesis, classification, and effect on drug metabolizing enzymes and transporters. Drug Metab Rev 49:197–211

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Conning DM, Phillips JC (1986) Comparative metabolism of BHA, BHT and other phenolic antioxidants and its toxicological relevance. Food Chem Toxicol 24:1145–1148

    Article  CAS  PubMed  Google Scholar 

  • Dasilva G, Pazos M, Garcia-Egido E, Gallardo JM, Rodriguez I, Cela R, Medina I (2015) Healthy effect of different proportions of marine omega-3 PUFAs EPA and DHA supplementation in Wistar rats: lipidomic biomarkers of oxidative stress and inflammation. J Nutr Biochem 26:1385–1392

    Article  CAS  PubMed  Google Scholar 

  • Debib A, Dueñas M, Boumediene M, Mothana RA, Latifa A, Tir-Touil MA (2016) Synergetic hepatoprotective effect of phenolic fractions obtained from Ficus carica dried fruit and extra virgin olive oil on CCL4-induced oxidative stress and hepatotoxicity in rats. J Food Biochem 40:507–516

    Article  CAS  Google Scholar 

  • Erol-Dayi O, Arda N, Erdem G (2012) Protective effects of olive oil phenolics and gallic acid on hydrogen peroxide-induced apoptosis. Eur J Nutr 51:955–960

    Article  CAS  PubMed  Google Scholar 

  • Ezraty B, Vergnes A, Banzhaf M, Duverger Y, Huguenot A, Brochado AR, Su SY, Espinosa L, Loiseau L, Py B, Typas A, Barras F (2013) Fe–S cluster biosynthesis controls uptake of aminoglycosides in a ROS-less death pathway. Science 340:1583–1587

    Article  CAS  PubMed  Google Scholar 

  • Ferramosca A, Di Giacomo M, Zara V (2017) Antioxidant dietary approach in treatment of fatty liver: new insights and updates. World J Gastroenterol 23:4146–4157

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Giordano E, Visioli F (2014) Long-chain omega-3 fatty acids: molecular bases of potential antioxidant actions. Prostaglandins Leukot Essent Fatty Acids 90:1–4

    Article  CAS  PubMed  Google Scholar 

  • Hussain T, Tan B, Yin Y, Blachier F, Tossou MC, Rahu N (2016) Oxidative stress and inflammation: what polyphenols can do for us? Oxid Med Cell Longev 2016:7432797

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Joo T-W, Hong S-H, Park S-Y, Kim G-Y, Jhoo J-W (2016) Antioxidant effects of eriodictyol on hydrogen peroxide-induced oxidative stress in HepG2 Cells. J Korean Soc Food Sci Nutr 45:510–517

    Article  Google Scholar 

  • Kanaan GN, Harper ME (2017) Cellular redox dysfunction in the development of cardiovascular diseases. Biochim Biophys Acta 11:2822–2829

    Article  CAS  Google Scholar 

  • Keren I, Wu Y, Inocencio J, Mulcahy LR, Lewis K (2013) Killing by bactericidal antibiotics does not depend on reactive oxygen species. Science 339:1213–1216

    Article  CAS  PubMed  Google Scholar 

  • Kim DO, Lee CY (2004) Comprehensive study on vitamin C equivalent antioxidant capacity (VCEAC) of various polyphenolics in scavenging a free radical and its structural relationship. Crit Rev Food Sci Nutr 44:253–273

    Article  CAS  PubMed  Google Scholar 

  • Kim D-W, Kim K-H, Hong J-K, Cho K-H, Sa S-J, Kim Y-M, Park J-C, Seol K-H (2014) Comparision of carcass characteristics, meat quality, and fatty acid profiles between Duroc and corssbred pigs (Duroc × Korean native pig). Korean J Agric Sci 41:425–431

    Article  Google Scholar 

  • Kozlov S, Afonin A, Evsyukov I, Bondarenko A (2017) Alzheimer’s disease: as it was in the beginning. Rev Neurosci 28(8):825–843

    Article  CAS  PubMed  Google Scholar 

  • Lee KJ, Jeong HG (2007) Protective effects of kahweol and cafestol against hydrogen peroxide-induced oxidative stress and DNA damage. Toxicol Lett 173:80–87

    Article  CAS  PubMed  Google Scholar 

  • Lee MY, Jung HR, Lim CH (2009) Analysis on developmental factors of the liver diseases in ultrasound diagnosis of healthcare. J Korean Soc Radiol Tech 32:79–86

    Google Scholar 

  • Lee YR, An YR, Park KJ, Sohn H, An DH, Kim SA (2013) Age and reproduction of the finless porpoises, Neophocaena asiaeorientalis, in the Yellow Sea, Korea. Anim Cells Syst 17:366–373

    Article  Google Scholar 

  • Liu Y, Imlay JA (2013) Cell death from antibiotics without the involvement of reactive oxygen species. Science 339:1210–1213

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Liu H, Liu X, Zhang C, Zhu H, Xu Q, Bu Y, Lei Y (2017) Redox imbalance in the development of colorectal cancer. J Cancer 8:1586–1597

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Monroig O, Tocher DR, Navarro JC (2013) Biosynthesis of polyunsaturated fatty acids in marine invertebrates: recent advances in molecular mechanisms. Mar Drugs 11:3998–4018

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Moon S-K, Lee D-S, Yoon H-D, Kim Y-K, Yoon NY, Kim I-S, Jeong B-Y (2012) Proximate and fatty acid compositions of three species of imported and domestic freshwater fishes. Korean J Fish Aquat Sci 45:612–618

    CAS  Google Scholar 

  • Parida AK, Das AB (2005) Salt tolerance and salinity effects on plants: a review. Ecotoxicol Environ Saf 60:324–349

    Article  CAS  PubMed  Google Scholar 

  • Park K-J, An Y-R, Lee Y-R, Park J-E, Moon D-Y, Choi S-G (2011) Feeding habits and consumption by finless porpoises (Neophocaena asiaeorientalis) in the Yellow Sea. Korean J Fish Aqua Sci 44:78–84

    Google Scholar 

  • Poole LG, Dolin CE, Arteel GE (2017) Organ-organ crosstalk and alcoholic liver disease. Biomolecules 7:62

    Article  CAS  PubMed Central  Google Scholar 

  • Rani V, Deep G, Singh RK, Palle K, Yadav UC (2016) Oxidative stress and metabolic disorders: pathogenesis and therapeutic strategies. Life Sci 148:183–193

    Article  CAS  PubMed  Google Scholar 

  • Redza-Dutordoir M, Averill-Bates DA (2016) Activation of apoptosis signalling pathways by reactive oxygen species. Biochim Biophys Acta 1863:2977–2992

    Article  CAS  PubMed  Google Scholar 

  • Sen CK, Packer L (1996) Antioxidant and redox regulation of gene transcription. FASEB J 10:709–720

    Article  CAS  PubMed  Google Scholar 

  • Siegel G, Ermilov E (2012) Omega-3 fatty acids: benefits for cardio-cerebro-vascular diseases. Atherosclerosis 225:291–295

    Article  CAS  PubMed  Google Scholar 

  • Simopoulos AP (1991) Omega-3 fatty acids in health and disease and in growth and development. Am J Clin Nutr 54:438–463

    Article  CAS  PubMed  Google Scholar 

  • Sohn H, Park KJ, An YR, Choi SG, Kim ZG, Kim HW, An DH, Lee YR, Park T-G (2012) Distribution of whales and dolphins in Korean waters based on a sighting survey from 2000 to 2010. Korean J Fish Aquat Sci 45:486–492

    Google Scholar 

  • Suarez-Jimenez GM, Lopez-Saiz CM, Ramirez-Guerra HE, Ezquerra-Brauer JM, Ruiz-Cruz S, Torres-Arreola W (2016) Role of endogenous and exogenous tocopherols in the lipid stability of marine oil systems: a review. Int J Mol Sci 17:1968

    Article  CAS  PubMed Central  Google Scholar 

  • Suzek H, Celik I, Dogan A, Yildirim S (2016) Protective effect and antioxidant role of sweetgum (Liquidambar orientalis) oil against carbon tetrachloride-induced hepatotoxicity and oxidative stress in rats. Pharm Biol 54:451–457

    Article  CAS  PubMed  Google Scholar 

  • Swanson D, Block R, Mousa SA (2012) Omega-3 fatty acids EPA and DHA: health benefits throughout life. Adv Nutr 3:1–7

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wang Y, Feinstein SI, Fisher AB (2008) Peroxiredoxin 6 as an antioxidant enzyme: protection of lung alveolar epithelial type II cells from H2O2-induced oxidative stress. J Cell Biochem 104:1274–1285

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Xiao X, Liu J, Hu J, Zhu X, Yang H, Wang C, Zhang Y (2008) Protective effects of protopine on hydrogen peroxide-induced oxidative injury of PC12 cells via Ca2+ antagonism and antioxidant mechanisms. Eur J Pharmacol 591:21–27

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This research was supported by grants to Dr. Dae Youn Hwang from the Korea Institute of Planning Evaluation for Technology of Food, Agriculture, Forestry and Fisheries (116027-032-HD030).

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Author notes

  1. Hyun Keun Song and DaeYoun Hwang contributed equally to this work.

Authors and Affiliations

  1. Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, 50 Cheonghak-ri, Samnangjin-eup, Miryang-si, Gyeongsangnam-do, 50463, South Korea

    Hye Ryeong Kim, Ji Eun Kim, Woo Bin Yun, Mi Rim Lee, Jun Young Choi, Jin Ju Park, Bo Ram Song & Dae Youn Hwang

  2. Biomedical Science Institute, Changwon National University, 20 Changwondae-ro Uichang-gu, Changwon-si, Gyeongsangnam-do, 51140, South Korea

    Hyun Keun Song

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  1. Hye Ryeong Kim
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Correspondence to Dae Youn Hwang.

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Kim, H.R., Kim, J.E., Yun, W.B. et al. Protective effect of oil extracted from Neophocaena asiaeorientalis against hydgrogen peroxide-induced oxidative stress in HepG2 cells. Fish Sci 85, 867–876 (2019). https://doi.org/10.1007/s12562-019-01329-9

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