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Cloning of the ANT gene and its expression profiles at different developmental stages and post-molting times in the ridgetail white prawn Exopalaemon carinicauda

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Abstract

To investigate the roles of the ANT gene, which codes for adenine nucleotide translocase (ANT) during crustacean development, a full-length cDNA sequence of EcANT in the ridgetail white prawn Exopalaemon carinicauda, was cloned, and its expression profile was analyzed at different developmental stages and post-molting times. The EcANT gene (GenBank accession number: KP892663) contained an open reading frame of 924 bp encoding a 307 amino acid protein with a theoretical size of about 33.42 kDa and a predicted isoelectric point of 9.77. Tissue expression analysis revealed that EcANT was mainly expressed in muscle and its expression level tended to increase with the developmental stages. In addition, the expression level of EcANT after molting increased following the lengthening of post-molting time. Our results suggest that EcANT is an important gene related to the growth and development of E. carinicauda.

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Acknowledgements

This project was Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), Open-end Funds of Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology (2015HS001), the Qing Lan Project, “521 Project”for scientific research of Lianyungang City and Enterprise-university-research institute cooperation funds of Lianyungang City (CXY1517).

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Authors and Affiliations

  1. Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, 59 Cangwu Road, Lianyungang, 222005, Jiangsu, People’s Republic of China

    Huan Gao, Bei Xue, Lian Zhao, Xiaofang Lai, Binlun Yan & Hanliang Cheng

  2. Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Lianyungang, 222005, China

    Huan Gao, Xiaofang Lai, Binlun Yan & Hanliang Cheng

  3. Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, 210014, China

    Huan Gao, Xiaofang Lai, Binlun Yan & Hanliang Cheng

  4. Zhejiang Institute of Freshwater Fisheries, Huzhou, 313001, Zhejiang, China

    Qian Pan

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  1. Huan Gao
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Correspondence to Huan Gao.

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Gao, H., Xue, B., Zhao, L. et al. Cloning of the ANT gene and its expression profiles at different developmental stages and post-molting times in the ridgetail white prawn Exopalaemon carinicauda . Fish Sci 83, 553–561 (2017). https://doi.org/10.1007/s12562-017-1094-0

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