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Production of an antibacterial substance by Bacillus mojavensis strain F412 isolated from a Myanmar shrimp product fermented with boiled rice

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  • Food Science and Technology
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Abstract

An antibacterial-substance-producing bacterium, namely, strain F412, was isolated from a traditional Myanmar shrimp product fermented with boiled rice. It was a gram-positive, spore-forming, and rod-shaped bacterium, and identified as Bacillus mojavensis on the basis of the gyrA sequence. The antibacterial substance of this strain was partially purified from a culture supernatant using two steps of column chromatography. This substance was found to be widely effective against gram-positive bacteria, including Listeria monocytogenes. The antibacterial activity of this substance was not susceptible to treatments with several proteolytic enzymes. The antibacterial activity gradually decreased with increasing treatment temperature, but it remained even after heating for 15 min at 121 °C. This antibacterial substance showed different molecular weights, as shown by the results of gel filtration and electrophoresis analyses. Staining results after electrophoresis suggest that the antibacterial substance might be a glycopeptide with an estimated molecular weight between 3.5 and 8.5 kDa. From the decrease in optical density of a culture of the L. monocytogenes treated with this antibacterial substance, it was suggested that this substance might have bacteriolytic activity.

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Acknowledgments

This study was supported by the Japanese Government (MONBUKAGAKUSHO) Scholarship program. The authors are grateful to Y. Watabe in our laboratory for technical assistance.

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Authors and Affiliations

  1. Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo, 108-8477, Japan

    Nant Kay Thwe Moe, Kousuke Suzuki, Ryosuke Nakai, Takeshi Terahara, Chiaki Imada & Takeshi Kobayashi

  2. Department of Fisheries, Ministry of Livestock, Fisheries and Rural Development, Bayintnaung Road, West Gyo Gone, Insein Township, Yangon, Myanmar

    Nant Kay Thwe Moe & Su Myo Thwe

Authors
  1. Nant Kay Thwe Moe
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  2. Su Myo Thwe
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  3. Kousuke Suzuki
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  4. Ryosuke Nakai
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  5. Takeshi Terahara
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  6. Chiaki Imada
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  7. Takeshi Kobayashi
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Correspondence to Takeshi Kobayashi.

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Moe, N.K.T., Thwe, S.M., Suzuki, K. et al. Production of an antibacterial substance by Bacillus mojavensis strain F412 isolated from a Myanmar shrimp product fermented with boiled rice. Fish Sci 81, 795–802 (2015). https://doi.org/10.1007/s12562-015-0878-3

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  • DOI: https://doi.org/10.1007/s12562-015-0878-3

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