Abstract
Tubulins are essential proteins, which are conserved across all eukaryotic species. They polymerize to form microtubules, cytoskeletal components of paramount importance for cellular mechanics. The microtubules combine an extraordinarily high flexural rigidity and a non-equilibrium behavior, manifested in their intermittent assembly and disassembly. These chemically fueled dynamics allow microtubules to generate significant pushing and pulling forces at their ends to reposition intracellular organelles, remodel membranes, bear compressive forces, and transport chromosomes during cell division. In this article, we review classical and recent studies, which have allowed the quantification of microtubule-generated forces. The measurements, to which we owe most of the quantitative information about microtubule forces, were carried out in biochemically reconstituted systems in vitro. We also discuss how mathematical and computational modeling has contributed to the interpretations of these results and shaped our understanding of the mechanisms of force production by tubulin polymerization and depolymerization.
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Acknowledgements
We thank J. R. McIntosh for the critical reading of the manuscript and helpful suggestions.
Funding
Analysis of in vitro studies of microtubules was supported by the Russian Science Foundation grant # 23-74-00007, https://rscf.ru/project/23-74-00007/. Analysis of mathematical and computational models was supported by the Scientific and Educational Mathematical Center “Sofia Kovalevskaya Northwestern Center for Mathematical Research” (agreement no. 075-02-2023-937, 16.02.2023).
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Gudimchuk, N.B., Alexandrova, V.V. Measuring and modeling forces generated by microtubules. Biophys Rev 15, 1095–1110 (2023). https://doi.org/10.1007/s12551-023-01161-7
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DOI: https://doi.org/10.1007/s12551-023-01161-7