Abstract
The preservation of foods at low temperatures is a well-established concept. While conventional methods of food freezing rely on the isobaric (constant pressure) approach, they often result in a series of irreversible changes that can significantly hamper the quality of frozen foods. In recent years, taking its roots from the biomedical industry, isochoric (constant volume) freezing is gaining both research and commercial interest as an effective method of food preservation. The focus of this review is to present the state of the art of isochoric freezing of foods, highlighting the underlying mechanisms that make it unique, and understanding its impact on food quality, considering reports published in the past decade. An exclusive section is dedicated to its non-food applications, and this work also provides insights into the costs and economics of the process. Importantly, as this is an emerging area, the review concludes by highlighting the challenges and provides perspectives on the directions for future research.
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References
You Y, Kang T, Jun S (2020) Control of ice nucleation for subzero food preservation. Food Eng Rev. https://doi.org/10.1007/s12393-020-09211-6
Muthukumarappan K, Marella C, Sunkesula V (2019) Food freezing technology. Handb Farm, Dairy Food Mach Eng 389–415. https://doi.org/10.1016/b978-0-12-814803-7.00015-4
Rubinsky B, Perez PA, Carlson ME (2005) The thermodynamic principles of isochoric cryopreservation. Cryobiology 50:121–138. https://doi.org/10.1016/j.cryobiol.2004.12.002
Powell-Palm MJ, Rubinsky B (2019) A shift from the isobaric to the isochoric thermodynamic state can reduce energy consumption and augment temperature stability in frozen food storage. J Food Eng 251:1–10. https://doi.org/10.1016/j.jfoodeng.2019.02.001
Lyu C, Nastase G, Ukpai G et al (2017) Isochoric refrigeration of food products. https://doi.org/10.7287/peerj.preprints.2740v1
Preciado JA, Rubinsky B (2010) Isochoric preservation: a novel characterization method. Cryobiology 60:23–29. https://doi.org/10.1016/j.cryobiol.2009.06.010
Szobota SA, Rubinsky B (2006) Analysis of isochoric subcooling. Cryobiology 53:139–142. https://doi.org/10.1016/j.cryobiol.2006.04.001
Giwa S, Lewis JK, Alvarez L et al (2017) The promise of organ and tissue preservation to transform medicine. Nat Biotechnol 35:530–542. https://doi.org/10.1038/nbt.3889
Vries RJ, de Yarmush M, Uygun K (2019) Systems engineering the organ preservation process for transplantation. Curr Opin Biotechnol 58:192–201. https://doi.org/10.1016/j.copbio.2019.05.015
Bruinsma BG, Berendsen TA, Izamis M et al (2013) Determination and extension of the limits to static cold storage using subnormothermic machine perfusion. 36:775–780. https://doi.org/10.5301/ijao.5000250
Eltzschig HK, Eckle T (2011) Review Ischemia and reperfusion—from mechanism to translation. Nat Med 17:1391–1401. https://doi.org/10.1038/nm.2507
Yoshida K, Matsui Y, Wei T et al (1999) A novel conception for liver preservation at a temperature just above freezing point. J Surg Res 81:216–223. https://doi.org/10.1006/jsre.1998.5505
Tessier SN, Weng L, Moyo WD et al (2018) Effect of ice nucleation and cryoprotectants during high subzero-preservation in endothelialized microchannels. ACS Biomater Sci Eng 4:3006–3015. https://doi.org/10.1021/acsbiomaterials.8b00648
do Amaral MCF, Lee RE, Costanzo JP (2015) Hepatocyte responses to in vitro freezing and β-adrenergic stimulation: insights into the extreme freeze tolerance of subarctic Rana sylvatica. J Exp Zool Part A Ecol Genet Physiol 323:89–96. https://doi.org/10.1002/jez.1905
Storey KB (1999) Living in the cold: freeze-induced gene responses in freeze-tolerant vertebrates. Clin Exp Pharmacol Physiol 26:57–63. https://doi.org/10.1046/j.1440-1681.1999.02990.x
Layne JR, Costanzo JP, Lee RE (1998) Freeze duration influences postfreeze survival in the frog Rana sylvatica. J Exp Zool 280:197–201. https://doi.org/10.1002/(SICI)1097-010X(19980201)280:2<197::AID-JEZ11>3.0.CO;2-J
Bruinsma BG, Berendsen TA, Izamis ML et al (2015) Supercooling preservation and transplantation of the rat liver. Nat Protoc 10:484–494. https://doi.org/10.1038/nprot.2015.011
Berendsen TA, Bruinsma BG, Puts CF et al (2014) Supercooling enables long-term transplantation survival following 4 days of liver preservation. Nat Med 20:790–793. https://doi.org/10.1038/nm.3588
Taylor MJ, Weegman BP, Baicu SC, Giwa SE (2019) New approaches to cryopreservation of cells, tissues, and organs. Transfus Med Hemotherapy 46:197–215. https://doi.org/10.1159/000499453
Costanzo JP, Grenot C, Lee RE (1995) Supercooling, ice inoculation and freeze tolerance in the European common lizard, Lacerta vivipara. J Comp Physiol B 165:238–244. https://doi.org/10.1007/BF00260815
Fahy GM, Wowk B, Wu J et al (2004) Cryopreservation of organs by vitrification: perspectives and recent advances. Cryobiology 48:157–178. https://doi.org/10.1016/j.cryobiol.2004.02.002
Mikus H, Miller A, Nastase G et al (2016) The nematode Caenorhabditis elegans survives subfreezing temperatures in an isochoric system. Biochem Biophys Res Commun 477:401–405. https://doi.org/10.1016/j.bbrc.2016.06.089
Preciado J, Rubinsky B (2018) The effect of isochoric freezing on mammalian cells in an extracellular phosphate buffered solution. Cryobiology 82:155–158. https://doi.org/10.1016/j.cryobiol.2018.04.004
Wan L, Powell-Palm MJ, Lee C et al (2018) Preservation of rat hearts in subfreezing temperature isochoric conditions to – 8 °C and 78 MPa. Biochem Biophys Res Commun 496:852–857. https://doi.org/10.1016/j.bbrc.2018.01.140
Powell-Palm MJ, Zhang Y, Aruda J, Rubinsky B (2019) Isochoric conditions enable high subfreezing temperature pancreatic islet preservation without osmotic cryoprotective agents. Cryobiology 86:130–133. https://doi.org/10.1016/j.cryobiol.2019.01.003
Salinas-Almaguer S, Angulo-Sherman A, Sierra-Valdez FJ, Mercado-Uribe H (2015) Sterilization by cooling in isochoric conditions: the case of Escherichia coli. PLoS One 10:1–9. https://doi.org/10.1371/journal.pone.0140882
Powell-Palm MJ, Preciado J, Lyu C, Rubinsky B (2018) Escherichia coli viability in an isochoric system at subfreezing temperatures. Cryobiology 85:17–24. https://doi.org/10.1016/j.cryobiol.2018.10.262
Bridges DF, Bilbao-Sainz C, Powell-Palm MJ et al (2020) Viability of Listeria monocytogenes and Salmonella Typhimurium after isochoric freezing. J Food Saf 1–8. https://doi.org/10.1111/jfs.12840
Bilbao-Sainz C, Zhao Y, Takeoka G et al (2020) Effect of isochoric freezing on quality aspects of minimally processed potatoes. J Food Sci 00:1–9. https://doi.org/10.1111/1750-3841.15377
Năstase G, Lyu C, Ukpai G et al (2017) Isochoric and isobaric freezing of fish muscle. Biochem Biophys Res Commun 485:279–283. https://doi.org/10.1016/j.bbrc.2017.02.091
Bilbao-Sainz C, Sinrod AJG, Williams T et al (2020) Preservation of tilapia (Oreochromis aureus) fillet by isochoric (constant volume) freezing. J Aquat Food Prod Technol 00:1–12. https://doi.org/10.1080/10498850.2020.1785602
Bilbao-Sainz C, Sinrod A, Powell-Palm MJ et al (2019) Preservation of sweet cherry by isochoric (constant volume) freezing. Innov Food Sci Emerg Technol 52:108–115. https://doi.org/10.1016/j.ifset.2018.10.016
Bilbao-Sainz C, Sinrod AGJ, Dao L et al (2019) Preservation of spinach by isochoric (constant volume) freezing. Int J Food Sci Technol 0–2. https://doi.org/10.1111/ijfs.14463
Bilbao-Sainz C, Sinrod AJ, Dao L, Takeoka G, Williams T, Wood D, McHugh T (2021) Preservation of grape tomato by isochoric freezing. Food Res Int 110228. https://doi.org/10.1016/j.foodres.2021.110228
Ukpai G, Năstase G, Şerban A, Rubinsky B (2017) Pressure in isochoric systems containing aqueous solutions at subzero centigrade temperatures. PLoS One 12:1–16. https://doi.org/10.1371/journal.pone.0183353
Zhang Y, Ukpai G, Grigoropoulos A et al (2018) Cryobiology isochoric vitrification: an experimental study to establish proof of concept 83:48–55. https://doi.org/10.1016/j.cryobiol.2018.06.005
Powell-Palm MJ, Koh-Bell A, Rubinsky B (2020) Isochoric conditions enhance stability of metastable supercooled water isochoric conditions enhance stability of metastable supercooled water. Appl Phys Lett 123702. https://doi.org/10.1063/1.5145334
Powell-Palm MJ, Aruda J, Rubinsky B (2019) Thermodynamic theory and experimental validation of a multiphase isochoric freezing process. J Biomech Eng 141. https://doi.org/10.1115/1.4043521
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Nida, S., Moses, J.A. & Anandharamakrishnan, C. Isochoric Freezing and Its Emerging Applications in Food Preservation. Food Eng Rev 13, 812–821 (2021). https://doi.org/10.1007/s12393-021-09284-x
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DOI: https://doi.org/10.1007/s12393-021-09284-x