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A SHORTROOT-Mediated Transcriptional Regulatory Network for Vascular Development in the Arabidopsis Shoot

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Abstract

In plants, vascular development is a dynamic process that integrates extrinsic and intrinsic factors. In Arabidopsis (Arabidopsis thaliana), SHORTROOT (SHR) has been known to play key roles in regulating cell division and differentiation in root vascular development. However, the role of SHR in the shoot vasculature remains unknown. Here, we employed various experimental approaches to unravel SHR’s role in shoot vascular development. In hypocotyls and inflorescence stems, shr exhibited reduced cell numbers in the (pro)cambium. As a result, the daughter cells that would be incorporated into the phloem and xylem would become a shortage of supply, thereby causing precocious differentiation. By expression profiling, we identified a putative SHR-mediated transcriptional regulatory network (TRN), which includes a subset of the ETHYLENE RESPONSE FACTOR (ERF) family. Among these, we found that SHR directly regulated expression of ERF018, whose expression domains were largely overlapped with those of SHR. Additionally, we found that ERF018 overexpressors (ERF018-OXs) showed a reduction in cambium cell division in hypocotyls. Interestingly, the level of SHR transcripts was elevated in ERF018-OX hypocotyls. Taken together, our results provide insights into the previously uncharacterized role of SHR, implying that maintaining homeostasis of key regulators’ levels plays an important role in shoot vascular development.

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Acknowledgements

This work was supported by the Konkuk University Research Fund 2018. We are grateful to Seungwoo Kim for technical assistance. We also thank Dr. Philip Benfey and Arabidopsis Biological Resource Center (ABRC) for plant lines.

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Author notes

  1. Eun Kyung Yoon

    Present address: Plant Biotechnology Research Center, Ghent University Global Campus, Incheon, 21985, Korea

  2. Souvik Dhar

    Present address: School of Biological Sciences, Seoul National University, Seoul, 08826, Korea

  3. Kangseok Ko and Eun Kyung Yoon have contributed equally to this work.

Authors and Affiliations

  1. Department of Systems Biotechnology, Konkuk University, Seoul, 05029, Korea

    Kangseok Ko, Eun Kyung Yoon, Souvik Dhar, Jiyeong Oh & Jun Lim

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  1. Kangseok Ko
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  2. Eun Kyung Yoon
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  3. Souvik Dhar
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  4. Jiyeong Oh
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  5. Jun Lim
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Contributions

KK, EKY, and JL conceived and designed the research plans. KK and EKY performed experiments. EKY analyzed microarray data. SD conducted ChIP-qPCR. JO performed plant work including genotyping. KK, EKY, and JL wrote the manuscript with contributions of all the authors.

Corresponding author

Correspondence to Jun Lim.

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All the authors agreed on the contents of the paper and the authors declare no conflict of interest.

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Ko, K., Yoon, E.K., Dhar, S. et al. A SHORTROOT-Mediated Transcriptional Regulatory Network for Vascular Development in the Arabidopsis Shoot. J. Plant Biol. 65, 341–355 (2022). https://doi.org/10.1007/s12374-022-09355-4

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  • DOI: https://doi.org/10.1007/s12374-022-09355-4

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