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Identification of Near Homozygous Inbred Lines for Developing Hybrid Populations to Explore the Possibility of True Seed-Based Sugarcane Cultivation

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Abstract

Identification of homozygous lines is a prerequisite for commercial sugarcane cultivation through true seed, which will bring a transformational change in sugarcane agriculture. In order to explore this possibility, several commercial canes were selfed and the selfs were evaluated for economic traits and molecular similarity. Among the families, Co 1148 family contained advanced generation selfs, while others were of early generations. The families Co 1148, Co 86032, Co 99008 recorded the least variability for cane yield and quality traits, while Co 775 families exhibited the highest variability. On genotyping with eleven sequence tagged microsatellite markers and subjecting the data to STRUCTURE analysis revealed 9, 4, 4 and 2 subpopulations in Co 1148, Co 86032, Co 99008 and Co 775 families, respectively. In each family, clone(s) amplifying least number of markers within each allele and having highest genomic differentiation in relation to the parent are considered as relatively homozygous types. One such identified sixth generation self, viz. 1148-13-11-2-237-2-61 was selfed and crossed with other identified near homozygous lines, and the progenies were compared for their homogeneity. The hybrid progeny populations recorded lower coefficient of variation and exhibited high hybrid vigour over the corresponding selfed progenies. The results implied the scope of hybrid breeding and true seed production in sugarcane through developing homozygous selfs. The identified near homozygous selfs need to be further advanced and investigated for their utilisation as parents in sugarcane true seed production programme.

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References

  • Adamack, A.T., and B. Gruber. 2014. PopGenReport: Simplifying basic population genetic analyses in R. Methods in Ecology and Evolution 5(4): 384–387. https://doi.org/10.1111/2041-210X.12158.

    Article  Google Scholar 

  • Doyle, J.J., and J.L. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11–15.

    Google Scholar 

  • Durai, A.A., G. Hemaprabha, R. Kumar, and K. Mohanraj. 2012. Effects of selfing on important economic traits in sugarcane (Saccharum spp.). Journal of Sugarcane Research 2(2): 29–33.

    Google Scholar 

  • Durai, A.A., and G. Hemaprabha. 2016. Heterosis and combining ability of sugarcane inbredsfor early stage selection traits. Journal of Sugarcane Research 6(1): 27–34.

    Google Scholar 

  • Durai, A.A., G. Hemaprabha, V.R. Viola, T.S.S. Padmanaban, and K. Lakshmi. 2019. Studies on economic traits in successive generations of selfing in sugarcane (Saccharum spp.). Journal of Sugarcane Research 9(2): 119–129.

    Article  Google Scholar 

  • Earl, D.A., and B.M. Von Holdt. 2012. STRUCTURE HARVESTER: A website and program for visualizing STRUCTURE output and implementing the Evanno method. Conservation Genetics Resources 4(2): 359–361. https://doi.org/10.1007/s12686-011-9548-7.

    Article  Google Scholar 

  • Evanno, G., S. Regnaut, and J. Goudet. 2005. Detecting the number of clusters of individuals using the software STRUCTURE: A simulation study. Molecular Ecology 14(8): 2611–2620.

    Article  CAS  Google Scholar 

  • Ethirajan, S.A., P.N. Santhakumariamma, and N. Velayutham. 1978. Studies on successive inbred generations of selected sugarcane cultivars. Proceedings of the XVI International Society of Sugarcane Technologists Congress, Sao Paulo, 269–273.

  • Ferreira, F.M., M.H.P. Barbosa, R.D. de Castro, L.A. Peternelli, and C.D. Cruz. 2005. Effects of inbreeding on the selection of sugar cane clones. Crop Breeding and Applied Biotechnology 5: 174–182.

    Article  Google Scholar 

  • Goudet, J. and T. Jombart. 2020. hierfstat: Estimation and Tests of Hierarchical F-statistics. R package version 0.5-7. https://CRAN.R-project.org/package=hierfstat.

  • Hemaprabha, G., U.S. Natarajan, N. Balasundaram, and N. Subramonian. 2000. Quantification of genetic similarity among inbred progenies and identification of desirable parents for population development in sugarcane (Saccharum spp.) through isozyme analysis, 16–18. Sugar Cane International.

    Google Scholar 

  • Hemaprabha, G., U.S. Natarajan, and N. Balasundaram. 2003. Breeding behaviour for juice quality through selfing and progeny evaluation in hybrid clones and inbred derivatives of sugarcane (Saccharum spp.). Sugar Tech 5(3): 177–200.

    Article  CAS  Google Scholar 

  • Hemaprabha, G., and N.K. Singh. 2007. Evaluation of parental value of selfs in sugarcane (Saccharum spp.) breeding. The Indian Journal of Genetics and Plant Breeding 67(3): 248–253.

    CAS  Google Scholar 

  • Hogarth, D.M. 1980. The effect of accidental selfing on the analysis of a diallel crosses with sugarcane. Euphytica 29: 737–746.

    Article  Google Scholar 

  • Jombart, T. 2008. adegenet: A R package for the multivariate analysis of genetic markers. Bioinformatics 24: 1403–1405. https://doi.org/10.1093/bioinformatics/btn129.

    Article  CAS  Google Scholar 

  • Kamvar, Z.N., J.F. Tabima, and N.J. Grünwald. 2014. Poppr: An R package for genetic analysis of populations with clonal, partially clonal, and/or sexual reproduction. PeerJ 2: e281. https://doi.org/10.7717/peerj.281.

    Article  Google Scholar 

  • Mettler, L.E., and T.G.C. Gregg. 1973. Genetica de Populacoes Poligono/EDUSP, Sau Paula, 262.

  • Neel, J.V., and E.A. Thompson. 1978. Founder effect and number of private polymorphisms observed in Amerindian tribes. Proceedings of the National Academy of Sciences of the United States of America 75: 1904–1908.

    Article  CAS  Google Scholar 

  • Panje, R.L., and A.S. Ethirajan. 1959. Studies on Saccharum spontaneum—Preliminary studies in inbreeding. Proceedings of the X International Society of Sugar Cane Technologists Congress.

  • Paradis, E. 2010. pegas: An R package for population genetics with an integrated-modular approach. Bioinformatics 26: 419–420.

    Article  CAS  Google Scholar 

  • Pritchard, J.K., M. Stephens, and P. Donnelly. 2000. Inference of population structure using multilocus genotype data. Genetics 155: 945–959.

    Article  CAS  Google Scholar 

  • Ram, B., G. Hemaprabha, and M.N. Premachandran. 2015. Sugarcane cultivation through true seed—A historic step towards changing mode of transportation of sugarcane seed from truck to pocket. SBI Newsletter 35(3): 1–2.

    Google Scholar 

  • Shanthi, R.M., and S. Alarmelu. 2011. Parental stocks for early high sugar content through simple recurrent selection. SBI News 31(4): 2–3.

    Google Scholar 

  • Silva, M.A., and P.S. Gonclaves. 2011. Inbreeding in sugarcane varieties. Ciencia Rural, Santa Maria 41(4): 580–586.

    Article  Google Scholar 

  • Slatkin, M. 1985. Rare alleles as indicators of gene flow. Evolution 39: 35–65.

    Article  Google Scholar 

  • Stevenson, G.C. 1953. The use of selfing and inbreeding with sugarcane. Proceedingsof the VIII International Society of SugarCane Technologists, 1954, British West Indies, 509–520.

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Authors and Affiliations

  1. Division of Crop Improvement, ICAR-Sugarcane Breeding Institute, Coimbatore, 631007, Tamil Nadu, India

    T. Lakshmi Pathy, V. Vinu, A. Annadurai, Bakshi Ram & G. Hemaprabha

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  1. T. Lakshmi Pathy
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  2. V. Vinu
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  3. A. Annadurai
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  4. Bakshi Ram
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  5. G. Hemaprabha
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Correspondence to G. Hemaprabha.

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Pathy, T.L., Vinu, V., Annadurai, A. et al. Identification of Near Homozygous Inbred Lines for Developing Hybrid Populations to Explore the Possibility of True Seed-Based Sugarcane Cultivation. Sugar Tech 25, 308–319 (2023). https://doi.org/10.1007/s12355-022-01144-0

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