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Yellow Canopy Syndrome (YCS) in Sugarcane is Associated with Altered Carbon Partitioning in the Leaf

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Abstract

Understanding the metabolic and gene expression changes that accompany the expression of Yellow Canopy Syndrome (YCS) in sugarcane is important and could greatly assist in developing management strategies as well as in the identification of potential causal factors. Leaves representing two stages of development (leaves 4 and 6) from YCS symptomatic and YCS asymptomatic plants, from two seasons, were analysed using gas chromatography linked to mass spectrometry. More than 200 metabolites were detected in the leaf samples, and 84 of these could be identified. The results revealed intrinsic differences (p = 0.05) between the metabolomes of the YCS symptomatic and asymptomatic plants. It was evident that significant metabolic changes occurred well before the development of leaf yellowing. The major metabolic changes were associated with sugar metabolism, the pentose phosphate cycle, and phenylpropanoid and α-ketoglutarate metabolism. The diurnal changes of sucrose concentrations (low in the morning and high at the end of the day) are absent in the YCS symptomatic plants even before symptom expression. Comparing the leaf transcriptomes of the symptomatic and asymptomatic plants shows that a complex network of changes in gene expression underpins the observed changes in the metabolome.

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Funding

This study was funded by Sugar Research Australia (Grant Number 2015016).

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Authors and Affiliations

  1. Sugar Research Australia Limited, PO Box 86, Indooroopilly, 4068, Australia

    Annelie Marquardt, Gerard Scalia, Kate Wathen-Dunn & Frederik C. Botha

  2. Queensland Alliance for Agriculture and Food Innovation (QAAFI), University of Queensland, St Lucia, 4072, Australia

    Annelie Marquardt & Frederik C. Botha

Authors
  1. Annelie Marquardt
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  2. Gerard Scalia
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  3. Kate Wathen-Dunn
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  4. Frederik C. Botha
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Correspondence to Frederik C. Botha.

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Marquardt, A., Scalia, G., Wathen-Dunn, K. et al. Yellow Canopy Syndrome (YCS) in Sugarcane is Associated with Altered Carbon Partitioning in the Leaf. Sugar Tech 19, 647–655 (2017). https://doi.org/10.1007/s12355-017-0555-1

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