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Modulation of salt stress in paddy field cyanobacteria with exogenous application of gibberellic acid: growth behavior and antioxidative status

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Abstract

The present study explores the possible function of gibberellic acid (GA: 20 µM) in reducing salt (NaCl) induced toxicity in two diazo-trophic cyanobacteria i.e. Nostoc muscorum and Phormidium foveolarum. The physiological and biochemical parameters viz. growth, photosynthetic pigments (chlorophyll a, carotenoids, and phycocyanin), photosynthetic and respiratory rates, oxidative stress biomarkers (superoxide radicle, hydrogen peroxide, and malondialdehyde contents) antioxidant activities (superoxide dismutase, peroxidase, catalase, and glutathione-S-transferase) and non-enzymatic antioxidants were studied under both the doses i.e. 40 mM (LC 10) and  mM (LC 30) of NaCl. The growth, photosynthetic pigments and photosynthetic rate were found to be declined under concentration-dependent manner of NaCl. Contrastingly, the respiratory rate, oxidative stress biomarkers, and the activity of antioxidant enzymes i.e. superoxide dismutase (SOD), peroxidases (POD), catalase (CAT), and glutathione-S-transferase (GST) together with contents of non-enzymatic antioxidants (proline and cysteine) were found to increase in the test cyanobacteria. PSII photochemistry in both the cyanobacteria was negatively affected showing an inhibitory effect of NaCl on JIP parameters, while an enhancement effect was noticed in the values related to energy flux parameters. Further, the addition of GA to the growth medium caused an alleviating effect as it completely mitigated NaCl toxicity induced by a lower dose i.e. 40 mM of NaCl, while it partially alleviated the growth and photosynthetic parameters of 80 mM NaCl stressed cyanobacteria. Supplementation of GA significantly reduced the contents of oxidative stress tested cyanobacteria due to an improved antioxidant system (increased activities of enzymatic and non-enzymatic antioxidants) as evident from the biochemical analysis. In brief, our findings reflect the possible role of GA as a potential modulator of salt toxicity.

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Acknowledgments

The authors are thankful to the Head, Department of Botany for providing necessary laboratory facilities. Anuradha Patel is thankful to NFO as ‘SRF (NFO-2015-17-OBC-UTT-41056)’ and Sanjesh Tiwari is thankful to Council for Scientific and Industrial Research (CSIR-UGC) New Delhi as ‘SRF (letter number 2121430412, EU-V)’ for providing financial assistance. The authors also acknowledge the Department of Science and Technology, FIST, New Delhi for providing the facilities to the Botany Department to carry out this work.

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  1. Anuradha Patel and Sanjesh Tiwari have contributed equally to this work.

Authors and Affiliations

  1. Ranjan Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad, Prayagraj, 211002, India

    Anuradha Patel, Sanjesh Tiwari & Sheo Mohan Prasad

  2. Department of Botany Dayalbagh Educational institute (Deemed University), Agra, 282005, India

    Anuradha Patel

  3. Department of Botany, Ghatsila College, Jamshedpur, East Singhbhum, Jharkhand, India

    Sanjesh Tiwari

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SMP, AP and ST designed the experiment AP and ST performed the experiment and calculated the data SMP, AP and ST wrote and read the manuscript.

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Correspondence to Sheo Mohan Prasad.

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Patel, A., Tiwari, S. & Prasad, S.M. Modulation of salt stress in paddy field cyanobacteria with exogenous application of gibberellic acid: growth behavior and antioxidative status. Physiol Mol Biol Plants 29, 51–68 (2023). https://doi.org/10.1007/s12298-022-01266-5

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