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Salt stress mitigation in Lathyrus cicera by combining different microbial inocula

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Abstract

Arid and semi-arid areas are considered vulnerable to various environmental constraints which are further fortified by climate change. Salinity is one of the most serious abiotic factors affecting crop yield and soil fertility. Till now, no information is available on the effect of salinity on development and symbiotic nitrogen (N2) fixation in the legume species Lathyrus cicera. Here, we evaluated the effect of different microbial inocula including nitrogen-fixing Rhizobium laguerreae, arbuscular mycorrhizal fungus (AMF) Rhizophagus irregularis, a complex mixed inoculum of AMF isolated from rhizospheric soil in “Al Aitha”, and various plant growth-promoting bacteria (PGPB) including Bacillus subtilus, Bacillus simplex and Bacillus megaterium combined with Rhizobium, the AMF consortium, or R. irregularis on alleviating salt stress in this legume. A pot trial was conducted to evaluate the ability of different microbial inocula to mitigate adverse effects of salinity on L. cicera plants. The results showed that salinity (100 mM NaCl) significantly reduced L. cicera plant growth. However, inoculation with different inocula enhanced plant growth and markedly promoted various biochemical traits. Moreover, the combined use of PGPB and AMF was found to be the most effective treatment in mitigating deleterious effects of salinity stress on L. cicera. In addition, this co-inoculation upregulated the expression of two marker genes (LcHKT1 and LcNHX7) related to salinity tolerance. Our findings suggest that the AMF/PGPB formulation has a great potential to be used as a biofertilizer to improve L. cicera plant growth and productivity under saline conditions.

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Acknowledgements

The authors thank Dr Maria Carlota VazPatto and Dr Carmen Santos for providing HKT1 and NHX7 gene sequences from their unpublished L. cicera transcriptomic databases. This research was funded by scholarships from the "International Relations Office Frau Veronika Favre", University of Fribourg and the Tuniso-Moroccan bilateral project (20/PRD03).

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  1. Laboratory of Legumes and Sustainable Agrosystems, Centre of Biotechnology of Borj-Cedria, BP 901, 2050, Hammam-Lif, Tunisia

    Takwa Gritli, Hatem Boubakri, Jihed Hsouna, Houda Ilahi & Bacem Mnasri

  2. Department of Biology, Rte. Albert-Gockel 3, CH- 1700, Fribourg, Switzerland

    Abdellatif Essahibi & Didier Reinhardt

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Gritli, T., Boubakri, H., Essahibi, A. et al. Salt stress mitigation in Lathyrus cicera by combining different microbial inocula. Physiol Mol Biol Plants 28, 1191–1206 (2022). https://doi.org/10.1007/s12298-022-01205-4

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