Skip to main content
SpringerLink
Log in

Oxidative stress, leaf photosynthetic capacity and dry matter content in young mangrove plant Rhizophora mucronata Lam. under prolonged submergence and soil water stress

  • Research Article
  • Published:
Physiology and Molecular Biology of Plants Aims and scope Submit manuscript

Abstract

Young plants of Rhizophora mucronata Lam. were tested for oxidative stress, photosynthetic capacity and dry matter accumulation under two abiotic stress conditions; prolonged submergence and soil water stress. The experiment of prolonged submergence was performed in field conditions with two treatment levels; 50% inundation (control) and 100% inundation levels. The experiment of soil water stress was conducted in a plant-house with four treatment levels, 100% water holding capacity (WHC) (control), 50% WHC, 25% WHC and high salinity (> 35 psu). The experimentation period was 18 months. According to the results, antioxidant activity was increased in the 100% inundation level in field conditions and in the 25% WHC, 50% WHC and high salinity levels in plant-house conditions. However, decreased radical scavenging capacity reflected by low 2,2-diphenyl-1-picrylhydrazyl (DPPH) and high IC50 values were only observed in the 25% and 50% WHCs. Plant cell membranes were highly damaged in the 25%, 50% WHCs and high salinity level and a significant decrease in photosynthetic capacity (~ 90% reduction) and in dry matter content of Rhizophora plants were also observed in the same treatment levels. It was recorded that a higher proportion of dry matter is allocated to the root system under the 100% inundation level and it may be an adaptation to keep up the standing stability. Although, the antioxidant and scavenging capacities of young Rhizophora plants have increased under abiotic stress conditions, oxidative stress and its associated impacts on leaf photosynthetic capacity and dry weight contents were unavoidable under persistence of the stress.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Ali AA, Alqurainy F (2006) Activities of antioxidants in plants under environmental stress. In: Motohashi N (ed) The lutein-prevention and treatment for diseases. Transworld Research Network, Trivandrum, pp 187–256

    Google Scholar 

  • Alongi DM (2002) Present state and future of the world’s mangrove forests. Environ Conserv 29:331–349

    Google Scholar 

  • Asada K (2006) Production and scavenging of reactive oxygen species in chloroplasts and their functions. Plant Physiol 141:391–396

    PubMed  PubMed Central  CAS  Google Scholar 

  • Asha KK, Mathew S, Lakshmanan PT (2012) Flavonoids and phenolics compounds in two mangrove species and their antioxidant property. Indian J Geo-Mar Sci 41(3):259–264

    CAS  Google Scholar 

  • Ashraf M (2009) Biotechnological approach of improving plant salt tolerance using antioxidants as markers. Biotechnol Adv 27:84–93

    PubMed  CAS  Google Scholar 

  • Ashraf M, Foolad MR (2007) Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ Exp Bot 59:206–216

    CAS  Google Scholar 

  • Ashraf M, Harris PJC (2013) Photosynthesis under stressful environments: an overview. Photosynthetica 51:163–190

    CAS  Google Scholar 

  • Aziz I, Khan MA (2001) Effect of seawater on the growth, ion content and water potential of Rhizophora mucronata Lam. J Plant Recourse 14:369–373

    Google Scholar 

  • Aziz I, Adnan MY, Khan F (2016) Metabolic implications of salt induced osmolyte accumulation in Avicennia marina. Pak J Bot 48(1):29–36

    Google Scholar 

  • Bajji M, Kinet JM, Lutts S (2001) The use of the electrolyte leakage method for assessing cell membrane stability as a water stress tolerance test in durum wheat. Plant Growth Regul 00:1–10

    Google Scholar 

  • Banerjee D, Chakrabarti S, Hazra AK, Banerjee S, Ray J, Mukherjee B (2008) Antioxidant activity and total phenolics of some mangroves in Sundarbans. Afr J Biotechnol 7(6):805–810

    Google Scholar 

  • Bartosz G (1997) Oxidative stress in plants. Acta Physiol Plant 19:47–64

    CAS  Google Scholar 

  • Benzine IFF, Szeto YT (1999) Total antioxidant capacity of teas by the ferric reducing antioxidant power assay. J Agric Food Chem 47:633–636

    Google Scholar 

  • Blois MS (1958) Antioxidant determination by use of stable free radical. Nature 181:1199–1200

    CAS  Google Scholar 

  • Colmer TD, Voesenek LACJ (2009) Flooding tolerance: suites of plant traits in variable environments. Funct Plant Biol 36:665–681

    PubMed  CAS  Google Scholar 

  • Cotelle N (2001) Role of flavonoids in oxidative stress. Curr Top Med Chem 1(6):569–590

    PubMed  CAS  Google Scholar 

  • Dai J, Mumper RJ (2010) Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules 15(10):7313–7352

    PubMed  PubMed Central  CAS  Google Scholar 

  • D’Archivio M, Filesi C, Di Benedetto R, Gargiulo R, Giovannini C, Masella R (2007) Polyphenols, dietary sources and bioavailability. Annali dellIstituto Superiore di Sanità 43(4):348–361

    Google Scholar 

  • Dionisio-Sese ML, Tobita S (1998) Antioxidant responses of rice seedlings to salinity stress. Plant Sci 135(1):1–9

    CAS  Google Scholar 

  • Dissanayake NP, Madarasinghe SK, Kodikara KAS, Jayatissa LP, Perera AJD, Koedam N, Dahdouh-Guebas F (2014) Preliminary study on the propagule dependency of Rhizophora seedlings. J Dept wildl Conserv 2:141–151

    Google Scholar 

  • Dissanayake NP, Kodikara KAS, Premachandra S, Jayatissa LP (2018) Structural and functional responses of xylem in Rhizophora mucronata Lam. seedlings under drought and hypersaline conditions. J Ruhuna Sci 9(1):13–31

    Google Scholar 

  • DM (Department of Meteorology), 383, Bauddhaloka Mawatha, Colombo 00700, Sri Lanka. Web access: http://www.meteo.gov.lk/. Accessed 18 June 2018

  • Faoro F, Iriti M (2005) Cell death behind invisible symptoms: early diagnosis of ozone injury. Biol Plant 49:585–592

    Google Scholar 

  • Field CD (1998) Rehabilitation of mangrove ecosystems: an overview. Mar Pollut Bull 37:383–392

    CAS  Google Scholar 

  • Flexas J, Bota J, Loreto F, Cornic G, Sharkey TD (2004) Diffusive and metabolic limitations to photosynthesis under drought and salinity in C3 plants. Plant Biol 6:269–279

    PubMed  CAS  Google Scholar 

  • Hameed A, Hussain T, Gulzar S, Aziz I, Gul B, Khan MA (2012) Salt tolerance of a cash crop halophyte Suaeda fruticosa: biochemical responses to salt and exogenous chemical treatments. Acta Physiol Plant 34:2331–2340. https://doi.org/10.1007/s11738-012-1035-6

    Article  CAS  Google Scholar 

  • Hernandez JA, Ohnos E, Corpas FJ, Sevilla L, del Rio LA (1995) Salt-induced oxidative stress in chloroplasts of pea plants. Plant Sci 105:151–167

    CAS  Google Scholar 

  • Hoppe-Speer SCL, Adams JB, Rajkaran A, Bailey D (2011) The response of the red mangrove Rhizophora mucronata Lam. to salinity and inundation in South Africa. Aquat Bot 95:71–76

    Google Scholar 

  • Hussain B, Ali B (2015) Leaf longevity in plants under water stress—a review. Indian J Plant Sci. ISSN: 2319–3824 (Online) An Open Access, Online. International Journal. Available at http://www.cibtech.org/jps.html

  • IUCN Sri Lanka (2009) An assessment of post tsunami restoration and conservation initiatives in coastal stretch of Matara, Sri Lanka

  • Jayatissa LP, Wickramasinghe WAADL, Dahdouh-Guebas F, Huxham M (2008) Interspecific variations in responses of mangrove seedlings to two contrasting salinities. Int Rev Hydrobiol 93(6):700–710

    Google Scholar 

  • Khanbabaee K, van Ree T (2001) Tannins: classification and definition. Nat Prod Rep 18:641–649

    PubMed  CAS  Google Scholar 

  • Kodikara KAS, Mukherjee N, Jayatissa LP, Dahdouh-Guebas F, Koedam N (2017) Have mangrove restoration projects worked? An in-depth study in Sri Lanka. Restor Ecol 25(5):705–716. https://doi.org/10.1111/rec.12492

    Article  Google Scholar 

  • Krauss KW, Doyle TW, Twilley RR, Rivera-Monroy VH, Sullivan JK (2006) Evaluating the relative contributions of hydroperiod and soil fertility on growth of south Florida mangroves. Hydrobiologia 569:311–324

    CAS  Google Scholar 

  • Krishnamoorthy M, Sasikumar JM, Shamna R, Pandiarajan C, Sofia P, Nagarajan B (2011) Antioxidant activities of bark extract from mangroves, Bruguiera cylindrica (L.) Blume and Ceriops decandra Perr. Indian J Pharmacol 43:557–562

    PubMed  PubMed Central  CAS  Google Scholar 

  • Król A, Amarowicz R, Weidner S (2014) Changes in the composition of phenolic compounds and antioxidant properties of grapevine roots and leaves (Vitis vinifera L.) under continuous of long-term drought stress. Acta Physiol Plant 36(6):1491–1499

    Google Scholar 

  • Lauri PE, Marceron A, Normand F, Dambreville A, Regnard JL (2014) Soil water deficit decreases xylem conductance efficiency relative to leaf area and mass in the apple. J Plant Hydrol 1:e0003

    Google Scholar 

  • Li W, Hydamaka AW, Lowry L, Beta T (2009) Comparison of antioxidant capacity and phenolic compounds of berries, chokecherry and seabuckthorn. Cent Eur J Biol 4(4):499–506. https://doi.org/10.2478/s11535-009-0041-1

    Article  CAS  Google Scholar 

  • Li W, Pickard MD, Beta T (2007) Effect of thermal processing on antioxidant properties of purple wheat bran. Food Chem 104:1080–1086

    CAS  Google Scholar 

  • Liu X, Huang B (2000) Heat stress injury in relation to membrane lipid peroxidation in creeping bentgrass. Crop Sci 40:503–510

    CAS  Google Scholar 

  • Malik NH, Mohdsin Z, Razak SBA, Ibrahim K, Zainol MK (2017) Antioxidative activities and flavonoids contents in leaves of selected mangrove species in Setiu Wetlands extracted using different solvents. J Sustain Sci Manag 2017:24–34

    Google Scholar 

  • McKersie BD, Bowley SR, Harjanto E, Leprince O (1996) Water-deficit tolerance and field performance of transgenic alfalfa overexpressing superoxide dismutase. Plant Physiol 11(1):1177–1181

    Google Scholar 

  • Meloni DA, Oliva MA, Martinez CA, Cambraia J (2003) Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Environ Exp Bot 49:69–76

    CAS  Google Scholar 

  • Munns R (2005) Comparative physiology of salt and water stress. Plant Cell Environment 25:239–250

    Google Scholar 

  • Munns R, Tester M (2008) Mechanisms of salinity tolerance. Annu Rev Plant Biol 59:651–681

    PubMed  CAS  Google Scholar 

  • Naidoo G (1985) Responses of the mangrove Rhizophora mucronata to high salinities and low osmotic potentials. S Afr J Bot 52:124–128

    Google Scholar 

  • Naidoo G (2006) Factors contributing to dwarfing in the mangrove Avicennia marina. Ann Bot 97:1095–1101

    PubMed  PubMed Central  CAS  Google Scholar 

  • Nandy DP, Ghose M (2001) Photosynthesis and water-use efficiency of some mangroves from Sundarbans, India. J Plant Biol 44:213–219

    Google Scholar 

  • National Resources Conservation Service (NRCS). Soil quality kit: guides for educators. United Sates Department of Agriculture (USDA), U.S.A. http://www.nrcs.usda.gov/wps/portal/nrcs/site/national/home/. Accessed 25 June 2017

  • Noctor G, Foyer CH (1998) Ascorbate and glutathione: keeping active oxygen under control. Annu Rev Plant Physiol Plant Mol Biol 49:249–279

    PubMed  CAS  Google Scholar 

  • Northeast Region Certified Crop Adviser (2010) Cornell University, Ithaca, NY 14850, United States. http://nrcca.cals.cornell.edu/nutrient/CA5/CA0539.php. Accessed 21 Apr 2017

  • Ou B, Hampsch-Woodill M, Prior RL (2001) Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe. J Agric Food Chem 49:4619–4626

    PubMed  CAS  Google Scholar 

  • Pezeshki SR, De Laune RD, Patrick JWH (1990) Differential response of selected mangroves to soil flooding and salinity: gas exchange and biomass. Can J For Res 20:869–874

    Google Scholar 

  • Poorter H, Nagel O (2000) The role of biomass allocation in the growth response of plants to different levels of light, CO2, nutrients and water: a quantitative review. Aust J Plant Physiol 27:595–607

    CAS  Google Scholar 

  • Ranasinghe P, Premakumara GAS, Wijayarathna CD, Ratnasooriya WD (2012) Antioxidant activity of Caryota urens L. (Kithul) Sap. Trop Agric Res 23(2):117–125

    Google Scholar 

  • Ravindran C, Naveenan T, Varatharajan GR, Rajasabapathy R, Meena RM (2012a) Antioxidants in mangrove plants and endophytic fungal associations. Bot Mar 55:269–279

    CAS  Google Scholar 

  • Ravindran C, Naveenan T, Varatharajan GR, Rajasabapathy R, Meena RM (2012b) Antioxidants in mangrove plants and endophytic fungal associations. Bot Mar 55(3):269–279

    CAS  Google Scholar 

  • Sairam RK, Veerabhadra RK, Srivastava GC (2002) Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Sci 163:1037–1046

    CAS  Google Scholar 

  • Shao HB, Li-Ye Chu LY, Jaleel CA, Zhao CX (2008) Water-deficit stress-induced anatomical changes in higher plants. C. R. Biol 331:215–225

    PubMed  Google Scholar 

  • Siddhuraju P, Becker K (2003) Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves. J Agric Food Chem 51(8):2144–2155

    PubMed  CAS  Google Scholar 

  • Singleton VL, Orthofer R, Lamuela-Raventos RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocaltue reagent. Methods Enzymol 299:152–178

    CAS  Google Scholar 

  • Smirnoff N (1993) The role of active oxygen in the response of plants to water deficit and desiccation. New Phytol 125:27–58

    CAS  PubMed  Google Scholar 

  • Thara MHMT, Dissanayake NP, Kodikara KAS, Perera AJD, Jayatissa LP (2016) Morphological and Anatomical responses of Rhizophora mucronata Lam. to water stress under greenhouse condition. In: Proceedings of 3rd Ruhuna international science and technology conference (RISTCON), vol 3, p 10

  • Thatoi HN, Patra JK, Das SK (2014) Free radical scavenging and antioxidant potential of mangrove plants: a review. Acta Physiol Plant 36(3):561–579

    CAS  Google Scholar 

  • Thitilertdecha N, Teerawutgulrag A, Kilburn JD, Rakariyatham N (2010) Identification of major phenolic compounds from Nephelium lappaceum L. and their antioxidant activities. Molecules 15:1453–1465

    PubMed  PubMed Central  CAS  Google Scholar 

  • Tomlinson PB (1994) The botany of mangroves. Cambridge University Press, Cambridge

    Google Scholar 

  • Valentao P, Fernandes E, Carvalho F, Andrade PB, Seabra RM, Bastos ML (2002) Antioxidative properties of cardoon (Cynara cardunculus L.) infusion against superoxide radical, hydroxyl radical, and hypochlorous acid. J Agric Food Chem 50:4989–4993

    PubMed  CAS  Google Scholar 

  • Verslues PE, Zhu JK (2005) Before and beyond ABA: upstream sensing and internal signals that determine ABA accumulation and response under abiotic stress. Biochem Soc Trans 33:375–379

    PubMed  CAS  Google Scholar 

Download references

Acknowledgement

This work described in this paper was supported by the VLIR-UOS-funded “Green Dyke project” (VLIR Ref.ZEIN2008PR347, Flemish Interuniversity Council – University Development Cooperation), University Grant commission, Sri Lanka (UGC/DRIC/UGC/DRIC/PG/2014AUG/RUH/02) and Faculty of Science, University of Ruhuna, Matara, Sri Lanka (RU/SF/RP/2015/02). We would also thank Mr. Douglas Thisera, SFFL, Pambala, for his field assistance.

Author information

Authors and Affiliations

  1. Department of Botany, University of Ruhuna, Wellamadama, Matara, Sri Lanka

    Kodikara Arachchilage Sunanda Kodikara, Jayatissa Loku Pullukuttige & Sanduni Kanishka Madarasinghe

  2. Herbal Technology Section, Industrial Technology Institute, Colombo, 7, Sri Lanka

    Ranasinghe Pathmasiri

  3. Institute of Sustainable Halophyte Utilization, University of Karachi, Karachi, Pakistan

    Aziz Irfan

  4. Laboratory of Plant Biology and Nature Management, Ecology and Biodiversity, Vrije Universiteit Brussel - VUB, Pleinlaan 2, 1050, Brussels, Belgium

    Kodikara Arachchilage Sunanda Kodikara, Dahdouh-Guebas Farid & Koedam Nico

  5. Laboratory of Systems Ecology and Resource Management, Department of Organism Biology, Faculty of Sciences, Université Libre de Bruxelles - ULB, Av. F.D. Roosevelt 50, CPi 2064/1, 1050, Brussels, Belgium

    Dahdouh-Guebas Farid

Authors
  1. Kodikara Arachchilage Sunanda Kodikara
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ranasinghe Pathmasiri
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Aziz Irfan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jayatissa Loku Pullukuttige
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sanduni Kanishka Madarasinghe
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Dahdouh-Guebas Farid
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Koedam Nico
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

KASK, PR, and IA designed the research; KASK and SKM performed the fieldwork; NK, FGD and, LPJ supervised the research; NK and SKM analyzed the data; KASK and PR wrote the paper; NK, LPJ, IA and FGD edited the manuscript.

Corresponding author

Correspondence to Kodikara Arachchilage Sunanda Kodikara.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 324 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kodikara, K.A.S., Pathmasiri, R., Irfan, A. et al. Oxidative stress, leaf photosynthetic capacity and dry matter content in young mangrove plant Rhizophora mucronata Lam. under prolonged submergence and soil water stress. Physiol Mol Biol Plants 26, 1609–1622 (2020). https://doi.org/10.1007/s12298-020-00843-w

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12298-020-00843-w

Keywords

Search

Navigation