Abstract
Nitrogen (N) is the basis of plant growth and development and, is considered as one of the priming agents to elevate a range of stresses. Plants use solar radiations through photosynthesis, which amasses the assimilatory components of crop yield to meet the global demand for food. Nitrogen is the main regulator in the allocation of photosynthetic apparatus which changes of the photosynthesis (Pn) and quantum yield (Fv/Fm) of the plant. In the present study, dynamics of the photosynthetic establishment, N-dependent relation with chlorophyll fluorescence attributes and Rubisco efficacy was evaluated in low-N tolerant (cv. CR Dhan 311) and low-N sensitive (cv. Rasi) rice cultivars under low-N and optimum-N conditions. There was a decrease in the stored leaf N under low-N condition, resulting in the decreased Pn and Fv/Fm efficiency of the plants through depletion in the activity and content of Rubisco. The Pn and Fv/Fm followed the parallel trend of leaf N content during low-N condition along with depletion of intercellular CO2 concentration and overall conductance under low-N condition. Photosynthetic saturation curve cleared abrupt decrease of effective quantum yield in the low-N sensitive rice cultivar than the low-N tolerant rice. Also, the rapid light curve highlighted the unacclimated regulation of photochemical and non-photochemical quenching in the low-N condition. The low-N sensitive rice cultivar triumphed non-photochemical quenching, whereas the low-N tolerant rice cultivar rose gradually during the light curve. Our study suggested that the quantum yield is the key limitation for photosynthesis in low-N condition. Regulation of Rubisco, photochemical and non-photochemical quenching may help plants to grow under low-N level.
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Abbreviations
- cv.:
-
Cultivar
- Ci :
-
Inter-cellular concentration of CO2
- ETR:
-
Electron transfer rate
- Fv/Fm :
-
Maximum photochemical quantum yield
- LNC:
-
Leaf nitrogen content
- NPQ:
-
Non-photochemical fluorescence quenching
- PAR:
-
Photosynthetically active radiation
- Pn :
-
Net photosynthesis
- PSC:
-
Photosynthesis saturation curve
- PSII:
-
Photosystem II
- qP:
-
Photochemical fluorescence quenching
- RLC:
-
Rapid light curve
- Rubisco:
-
Ribulose bisphosphate carboxylase/oxygenase
- SP:
-
Saturation pulse
- Y(II):
-
Effective photochemical quantum yield
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This work was supported by research Grants from DBT-NEWS-India-UK (BT/IN/UK-VNC/44/NR/2015-16).
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A. Ahmad conception and design of the study, acquisition of data, analysis, and interpretation of data, drafting the article and final approval of the submitted version. The author takes responsibility for the integrity of the article as a whole. aahmad.bo@amu.ac.in. A.Y. Tantray conception and design of the study, acquisition of data, interpretation of data, drafting the article, final approval. S.S. Bashir conception of the study, acquisition of data, analysis, and interpretation of data, final approval.
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Tantray, A.Y., Bashir, S.S. & Ahmad, A. Low nitrogen stress regulates chlorophyll fluorescence in coordination with photosynthesis and Rubisco efficiency of rice. Physiol Mol Biol Plants 26, 83–94 (2020). https://doi.org/10.1007/s12298-019-00721-0
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DOI: https://doi.org/10.1007/s12298-019-00721-0