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Evaluation of bioactive compounds and antioxidant potential of hydroethanolic extract of Moringa oleifera Lam. from Rajasthan, India

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Abstract

Moringa oleifera Lam., the miracle tree, is widely used as a traditional medicine. The analyses of phytochemicals and antioxidant potential of hydroethanolic extract of various plant parts of M. oleifera revealed that leaves possessed the highest content of total phenolics (9.58 mg/g), β-carotene (14.10 mg/g) and lycopene (2.60 mg/g). Flowers and bark showed the highest content of total flavonoids (3.5 mg/g) and anthocyanin (52.80 mg/g), respectively. Leaves also showed maximum antioxidant potential using nitric oxide scavenging assay (IC50 - 120 µg/ml) and deoxyribose degradation assay (IC50—178 µg/ml). Highest DPPH radical scavenging activity was observed in flowers (IC50—405 µg/ml). The GC–MS study revealed the presence of 29, 36 and 24 compounds in bark, leaf and flower, respectively. The major constituent identified were epiglobulol (41.68% in bark), phytol (23.54% in leaf) and β-sitosterol (15.35% in flower).The phytochemicals identified possess several therapeutic activity, including antioxidant potential, which was confirmed through earlier reports. Moreover, the presence of 1,1,3-triethoxubutane in all the plant parts analyzed, projects it as an important source of waste water treatment as hydrophobic modifiers.

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Acknowledgements

The authors thank Prof. Aditya Shastri, Vice Chancellor and Prof. Vinay Sharma, Head Department of Bioscience and Biotechnology, Banasthali Vidyapith, India, for providing the necessary facilities. Ms. Tanya Gupta is grateful to the DBT, Govt. of India for providing M.Sc. fellowship.

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  1. Department of Bioscience and Biotechnology, Banasthali University, P.O. Banasthali Vidyapith, 304022, Rajasthan, India

    Sharad Vats & Tanya Gupta

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Correspondence to Sharad Vats.

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Vats, S., Gupta, T. Evaluation of bioactive compounds and antioxidant potential of hydroethanolic extract of Moringa oleifera Lam. from Rajasthan, India. Physiol Mol Biol Plants 23, 239–248 (2017). https://doi.org/10.1007/s12298-016-0407-6

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  • DOI: https://doi.org/10.1007/s12298-016-0407-6

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