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Cross Sectional Study of Vitamin D Levels in Western Rajasthan and Meta-Analysis for Estimation of Vitamin D LevelsIn the PDF, in Header of all pages, Journal title should be abbreviated as "Ind J Clin Biochem"

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Abstract

Many studies showed Vitamin D deficiency is highly prevalent in healthy individuals. We planned to study the normal levels of Vitamin D in healthy individuals and make recommendation for defining deficiency of 25(OH)D in Indian population. Normal healthy subjects 18 to 60 years of age were included. Estimation of serum calcium, serum phosphorus, iPTH and bone alkaline phosphatase levels with vitamin D (25(OH)D) levels were done to study the normal 25(OH)D levels and make recommendation for defining deficiency of 25(OH)D in Indian population. Meta-analysis was performed of studies which estimated the mean vitamin D levels in healthy individuals. There was significant positive correlation of serum 25(OH)D levels with calcium levels (r = 0.148; p-value = 0.003). The normal mean values of 25(OH)D levels in total population was 13.5 ± 7.83 ng/ml, iPTH was 59.8 ± 28.84 pg/ml, bone ALP was 14.6 ± 6.66 microg/ml. The normal upper bound of 25(OH)D in 97.5% of total population in our study is less than 33.19 ng/ml. The normal upper bound of iPTH and bone ALP in 97.5% of total population in our study was less than 123.97 pg/ml and 32.19 microg/ml, respectively. Pooled analysis of 33 studies revealed overall mean 25(OH)D levels in total population to be 13.95 ng/ml (95%CI – 12.37–15.54). The concept of initializing treatment based on serum Vitamin D levels using the RDA (20ng/ml) and EAR (16ng/ml) values as “cutoff-points” is not recommended as per Institute of Medicine Committee on Dietary Reference Intakes, Washington DC. Vitamin D levels less than 12.5ng/ml in a symptomatic individual should be the sole criteria for treatment rather than Vitamin D levels alone.

Trial Registration: CTRI/2018/02/011820; CTRI/2018/02/011913.

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Acknowledgements

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Funding

All India Institute of Medical Sciences Jodhpur sponsored all the investigations.

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Authors and Affiliations

  1. Department of Pharmacology, All India Institute of Medical Sciences, Jodhpur, India

    Surjit Singh

  2. Central Institute of Orthopedics, VMMC and Safdarjung Hospital, New Delhi, India

    Divesh Jalan

  3. Department of Community Medicine and Family Medicine, All India Institute of Medical Sciences, Jodhpur, India

    Pankaj Bhardwaj

  4. Department of Biochemistry, All India Institute of Medical Sciences, Jodhpur, India

    Praveen Sharma

  5. Department of Orthopedics, All India Institute of Medical Sciences, Jodhpur, India

    Abhay Elhence

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  1. Surjit Singh
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  4. Praveen Sharma
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Contributions

Study design and planning of Study - All of the authors. Literature search - DJ, SS. Figures – SS, DJ. Tables – AE, DJ, SS. Data collection and analysis - SS, DJ. Data interpretation -, SS, DJ, AE. Writing - SS, AE, DJ. Corrections and Final approval of Manuscript - All of the authors. The corresponding author attests that all listed authors meet authorship criteria as per ICJME and that the manuscript is an honest, accurate, and transparent account of the study being reported.

Corresponding author

Correspondence to Abhay Elhence.

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Ethical approval and consent to participate

Ethical approval taken for study from All India Institute of Medical Sciences Jodhpur Ethics Committee - (IEC AIIMS/IEC/2017/737 and IEC AIIMS/IEC/2017/738).

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Not applicable.

Availability of supporting data

The datasets used and/or analysed during the current study are available from the corresponding author on request.

Competing Interests

Dr. SINGH has nothing to disclose. Dr Jalan D has nothing to disclose. Dr Bhardwaj P has nothing to disclose. Dr. Sharma P has nothing to disclose. Dr. Elhence A has nothing to disclose.

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Singh, S., Jalan, D., Bhardwaj, P. et al. Cross Sectional Study of Vitamin D Levels in Western Rajasthan and Meta-Analysis for Estimation of Vitamin D LevelsIn the PDF, in Header of all pages, Journal title should be abbreviated as "Ind J Clin Biochem". Ind J Clin Biochem (2022). https://doi.org/10.1007/s12291-022-01074-4

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