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Clinico-Hematological Profile and Copy Number Abnormalities in a Cohort of STIL-TAL1 and NUP214-ABL1 Positive Pediatric T-Cell Acute Lymphoblastic Leukemia

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Abstract

T cell acute lymphoblastic leukaemia (T-ALL) is a genetically heterogeneous and aggressive form of malignancy. Although a number of recurrent fusion genes are reported in T-ALL, their involvement in disease stratification and therapeutic intervention is still controversial. Considering the prognostic value of STIL-TAL1 fusion and tyrosine kinase inhibitor (TKI) based therapeutic potential of NUP214-ABL1, the present study aimed to investigate their frequency and clinical correlation in pediatric T-ALL cases. Our cohort consisted of 48 T-ALL pediatric cases (age ≤ 12 years) with a median age of 6 years and male to female ratio of 20.5:1. The median TLC of the study group was noted to be 220,000/ cu mm with a range from 26,810/cu mm to 785,430/cu mm. By MLPA and RT-PCR analysis we observed that 11/48 cases (23%) showed STIL-TAL1 fusion and 4/48 cases (8.3%) had NUP214-ABL1 fusion gene. Both of the fusion genes did not show any significant correlation with any of the clinico-hematological or treatment outcome parameters. However, upon analysis of copy number variations (CNVs) with other clinically relevant genes, we found significant correlation between LEF1 (p = 0.024) and PTEN (p = 0.049) gene deletions with STIL/TAL1 fusion in T-ALL patients. NUP214-ABL1 fusion gene did not reveal any association with either CNVs or with survival. Although limited with the small cohort size and follow up, our study supports the similar frequency of these fusions as compared to other Asian and Western studies and also highlights utility of MLPA technique as a good diagnostic modality to screen for both STIL-TAL1 and NUP214-ABL1 fusions in a single assay with additional data on secondary copy number changes.

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Funding

Special Research Grant, PGIMER.

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Author notes

  1. Nilamani Patra and Minu Singh have equally contributed to this work.

Authors and Affiliations

  1. Pediatric Hematology Oncology Unit, Department of Pediatrics, Postgraduate Institute of Medical Education and Research, Chandigarh, India

    Nilamani Patra, Minu Singh, Pankaj Sharma, Amita Trehan, Deepak Bansal & Prateek Bhatia

  2. Department of Hematology, Postgraduate Institute of Medical Education and Research, Chandigarh, India

    Shano Naseem

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  1. Nilamani Patra
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  2. Minu Singh
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  3. Pankaj Sharma
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  4. Amita Trehan
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  7. Prateek Bhatia
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Contributions

PB conceptualised the study; NP, MS, PS performed the experiments; PB, NP, MS analysed the data; PB, NP performed statistical analysis; AT, SN, DB provided patient sample and clinical data; PB, MS wrote and edited the manuscript.

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Correspondence to Prateek Bhatia.

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Patra, N., Singh, M., Sharma, P. et al. Clinico-Hematological Profile and Copy Number Abnormalities in a Cohort of STIL-TAL1 and NUP214-ABL1 Positive Pediatric T-Cell Acute Lymphoblastic Leukemia. Indian J Hematol Blood Transfus 37, 555–562 (2021). https://doi.org/10.1007/s12288-020-01394-6

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