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Platelet Function Analysis by Flowcytometry in Thrombocytopenic Trauma Patients

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Indian Journal of Hematology and Blood Transfusion Aims and scope Submit manuscript

Abstract

Hemostasis is dependent on sufficient quantity and quality of circulating functional platelets. Platelet function in trauma patients with thrombocytopenia and its impact on the clinical outcome is not adequately explored. Whether platelet dysfunction has a role in the pathogenesis of acute traumatic coagulopathy needs to be studied. Blood samples were collected from 70 thrombocytopenic trauma patients before transfusing platelets and assessed for platelet activation and platelet aggregation using flowcytometry. Primary outcome was in-hospital mortality. Platelet dysfunction was identified in 57.1% of thrombocytopenic trauma patients. A weak inverse correlation between percentage of activated platelets and APTT was observed (Spearman coefficient − 0.25, p = 0.03). A sensitivity and specificity of 66.6% was achieved for a cut off of ≤ 6.5% of platelet activation post trauma with ROC-AUC of 0.658 for identifying coagulopathy. No correlation with mortality however was observed (p > 0.05). Platelet dysfunction had a weak association with coagulopathy suggesting limited contribution of platelet dysfunction in pathogenesis of acute traumatic coagulopathy and warrants further research.

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Acknowledgement

This work was partially supported by the Indian Medical Council for Research [Grant No.3/2/Dec.2016/PG Thesis-HRD (11)].

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Authors and Affiliations

  1. Department of Laboratory Medicine, Jai Prakash Narayan Apex Trauma Centre, AIIMS, New Delhi, India

    Tapasyapreeti Mukhopadhyay, Arulselvi Subramanian, Venencia Albert, Anand Kumar & Shyam Prakash

  2. Department of Hematology (Retd), AIIMS, New Delhi, India

    Hara Prasad Pati

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  1. Tapasyapreeti Mukhopadhyay
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  2. Arulselvi Subramanian
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  3. Venencia Albert
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  4. Anand Kumar
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  5. Shyam Prakash
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  6. Hara Prasad Pati
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Correspondence to Arulselvi Subramanian.

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Mukhopadhyay, T., Subramanian, A., Albert, V. et al. Platelet Function Analysis by Flowcytometry in Thrombocytopenic Trauma Patients. Indian J Hematol Blood Transfus 37, 398–403 (2021). https://doi.org/10.1007/s12288-020-01349-x

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  • DOI: https://doi.org/10.1007/s12288-020-01349-x

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