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Downregulation of MIR100HG Induces Apoptosis in Human Megakaryoblastic Leukemia Cells

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Indian Journal of Hematology and Blood Transfusion Aims and scope Submit manuscript

Abstract

Long noncoding ribonucleic acids (lncRNAs) are ribonucleic acid (RNA) molecules longer than 200 nucleotides without protein-coding capacity. Several studies have shown that lncRNAs play a pivotal role in the initiation, maintenance, and progression of acute myeloid leukemia (AML), which could make them a promising candidate in the diagnosis and treatment of leukemia. Acute Megakaryoblastic leukemia (AMKL) is a rare form of AML with a poor prognosis and low survival. It has been reported that lncRNA MIR100HG is involved several types of malignancies. In the present study, MIR100HG was downregulated in a human acute megakaryoblastic leukemia cell line (M-07e) using Antisense LNA GapmeRs. In order to assess the expression level of MIR100HG, cell viability, apoptosis, and necrosis (late apoptosis), quantitative reverse transcription polymerase chain reaction (qRT-PCR), Methyl-thiazol Tetrazolium assay, AnnexinV, and propidium iodide staining was performed at different time points after the transfection. In addition, the expression level of TGFβ was evaluated by qRT-PCR. Our results revealed that inhibition of MIR100HG might serve as a new method for inhibition of the proliferation of AMKL cells and therefore, could be a promising approach in medicine for targeted therapy in AMKL.

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Acknowledgments

This study was conducted with the financial support of Isfahan University of Medical Sciences (IRAN) with Grant Number: 396739.

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Authors and Affiliations

  1. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Science, Isfahan, 81744-176, Iran

    Parisa Bagheri, Mohammadreza Sharifi & Ava Ghadiri

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  1. Parisa Bagheri
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  2. Mohammadreza Sharifi
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Correspondence to Mohammadreza Sharifi.

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The authors declare no conflict of interest.

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This study was approved by the local Ethics Committee of Isfahan University of Medical Sciences (IRAN); the current work was also approved by the relevant institutional and/or national research Ethics Committee and were performed in per under the ethical standards as laid down in the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.

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Bagheri, P., Sharifi, M. & Ghadiri, A. Downregulation of MIR100HG Induces Apoptosis in Human Megakaryoblastic Leukemia Cells. Indian J Hematol Blood Transfus 37, 232–239 (2021). https://doi.org/10.1007/s12288-020-01324-6

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  • DOI: https://doi.org/10.1007/s12288-020-01324-6

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