Abstract
Information which can be harvested from a biological sample has greatly improved with advancements in diagnostic technologies. However, in developing countries, the awareness about usefulness of bio-banking concept is lacking and centres which do offer it, depend mainly on − 20 or − 80 °C for sample storage due to lack of sophisticated infrastructure like vapour phase nitrogen storage preservation. Hence in these resource constraint settings, timely audit of quality of nucleic acids extractable from samples stored is of utmost importance. In this study, we explore the effect of − 20 °C storage over nucleic acids (DNA/RNA) isolated from blood samples of 180 patients with various leukaemia’s following a bio-banking initiative. We observed that the integrity and quality of both DNA and RNA were maintained in 70 and 80% samples respectively over time as reflected by their concentration measurements and inherent uniform expression of housekeeping gene GAPDH. Only 3.7% of the RNA samples and 4.2% of the DNA samples yielded very low concentrations despite minimizing processing and technical loss. In nutshell, audit of our biobank sample yield highlights that storage of blood samples at − 20 °C does not compromise the fidelity of nucleic acids for future diagnostic and research work in a resource constraint setting.
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Abbreviations
- B-ALL:
-
B cell acute lymphoblastic leukemia
- T-ALL:
-
T cell acute lymphoblastic leukemia
- AML:
-
Acute myeloid leukemia
- FFPE:
-
Formalin fixed paraffin embedded
- PBMC:
-
Peripheral blood mononuclear cells
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Acknowledgements
All faculty staff and DM residents of pediatric hematology oncology unit for providing samples for bio-banking of leukemia cases.
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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Informed consent was taken as a standard practice before biobanking of samples. The article is not funded by any support.
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Kumar, A., Singh, M., Bhatia, P. et al. Audit of Quality and Quantity of Nucleic Acid Yield from Pediatric Acute Leukemia Cases Following a Bio-banking Initiative. Indian J Hematol Blood Transfus 35, 77–82 (2019). https://doi.org/10.1007/s12288-018-0975-4
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DOI: https://doi.org/10.1007/s12288-018-0975-4