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MALDI-TOF MS: Foundations and a Practical Approach to the Clinically Relevant Filamentous Fungi Identification

  • Clinical Mycology Lab Issues (S Cordoba, Section Editor)
  • Published:
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Abstract

Purpose of Review

In this review, the applicability of matrix-assisted laser ionisation/desorption time-of-flight mass spectrometry (MALDI-TOF) to the identification of clinical relevant filamentous fungi is presented. In addition, the need to implement and validate in-house databases, to fill the gaps in commercial databases, and the importance of permanently updating spectra databases are topics addressed.

Recent Findings

MALDI-TOF MS is recognised as a powerful tool for discriminating complex and cryptic fungal species, as well as for strain typing of filamentous fungi of medical importance.

Summary

MALDI-TOF MS is a versatile technique and an undeniable tool for identification of filamentous fungi as part of the routine of a clinical mycology laboratory. The application of standardised procedures for the implementation and validation of the in-house databases, as well as for the identification of clinical isolates, is essential to obtain results that are comparable to those obtained through sequencing.

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Funding

This work was partially funded by the Comité para el Desarrollo de la Investigación, Universidad de Antioquia, (Medellín, Colombia) Grant No 2604, Laboratorio Clínico Synlab S. A. S, Colombia, Universidad de La Frontera (Temuco, Chile) through the Project DIUFRO PIA17‐0006. N. Lima acknowledges the support of FCT/Portugal under the scope of the strategic funding of UID/BIO/04469/2019 unit and BioTecNorte operation (NORTE-01–0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020—Programa Operacional Regional do Norte.

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Authors and Affiliations

  1. Laboratorio Clínico Synlab S. A. S, Bogotá, Colombia

    Juan C. Gómez-Velásquez & Iván L. Mojica-Figueroa

  2. Department of Chemical Science and Natural Resources, Universidad de La Frontera, 4811-230, Temuco, Chile

    Cledir Santos

  3. CEB-Biological Engineering Centre, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal

    Nelson Lima

  4. Grupo Académico de Epidemiologia Clínica, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia

    Ana C. Mesa-Arango

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  1. Juan C. Gómez-Velásquez
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Gómez-Velásquez, J.C., Mojica-Figueroa, I.L., Santos, C. et al. MALDI-TOF MS: Foundations and a Practical Approach to the Clinically Relevant Filamentous Fungi Identification. Curr Fungal Infect Rep 15, 162–170 (2021). https://doi.org/10.1007/s12281-021-00423-9

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