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Comparative Transcriptomic Analysis of Flagellar-Associated Genes in Salmonella Typhimurium and Its rnc Mutant

  • Microbial Physiology and Biochemistry
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Abstract

Salmonella enterica serovar Typhimurium (S. Typhimurium) is a globally recognized foodborne pathogen that affects both animals and humans. Endoribonucleases mediate RNA processing and degradation in the adaptation of bacteria to environmental changes and have been linked to the pathogenicity of S. Typhimurium. Not much is known about the specific regulatory mechanisms of these enzymes in S. Typhimurium, particularly in the context of environmental adaptation. Thus, this study carried out a comparative transcriptomic analysis of wild-type S. Typhimurium SL1344 and its mutant (∆rnc), which lacks the rnc gene encoding RNase III, thereby elucidating the detailed regulatory characteristics that can be attributed to the rnc gene. Global gene expression analysis revealed that the ∆rnc strain exhibited 410 upregulated and 301 downregulated genes (fold-change > 1.5 and p < 0.05), as compared to the wild-type strain. Subsequent bioinformatics analysis indicated that these differentially expressed genes are involved in various physiological functions, in both the wild-type and ∆rnc strains. This study provides evidence for the critical role of RNase III as a general positive regulator of flagellar-associated genes and its involvement in the pathogenicity of S. Typhimurium.

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Data availability

The raw RNA-seq data generated in this study have been deposited in the National Center for Biotechnology Information Sequence Read Archive, under the BioProject accession number PRJNA967189 (http://www.ncbi.nlm.nih.gov/sra/PRJNA967189).

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Acknowledgements

We thank Dr. Jinsoo Kim for his technical assistance. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (Ministry of Science and ICT; MSIT) (Grant No. 2022R1F1A1070791 and RS-2023-00210754).

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  1. Department of Microbiology, College of Medicine, Hallym University, Chuncheon, 24252, Republic of Korea

    Seungmok Han, Ji-Won Byun & Minho Lee

  2. Institute of Medical Science, College of Medicine, Hallym University, Chuncheon, 24252, Republic of Korea

    Minho Lee

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Han, S., Byun, JW. & Lee, M. Comparative Transcriptomic Analysis of Flagellar-Associated Genes in Salmonella Typhimurium and Its rnc Mutant. J Microbiol. 62, 33–48 (2024). https://doi.org/10.1007/s12275-023-00099-5

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