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LAMMER Kinase Governs the Expression and Cellular Localization of Gas2, a Key Regulator of Flocculation in Schizosaccharomyces pombe

  • Molecular Microbiology and Genomics
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Abstract

It was reported that LAMMER kinase in Schizosaccharomyces pombe plays an important role in cation-dependent and galactose-specific flocculation. Analogous to other flocculating yeasts, when cell wall extracts of the Δlkh1 strain were treated to the wild-type strain, it displayed flocculation. Gas2, a 1,3-β-glucanosyl transferase, was isolated from the EDTA-extracted cell-surface proteins in the Δlkh1 strain. While disruption of the gas2+ gene was not lethal and reduced the flocculation activity of the ∆lkh1 strain, the expression of a secreted form of Gas2, in which the GPI anchor addition sequences had been removed, conferred the ability to flocculate upon the WT strain. The Gas2-mediated flocculation was strongly inhibited by galactose but not by glucose. Immunostaining analysis showed that the cell surface localization of Gas2 was crucial for the flocculation of fission yeast. In addition, we identified the regulation of mbx2+ expression by Lkh1 using RT-qPCR. Taken together, we found that Lkh1 induces asexual flocculation by regulating not only the localization of Gas2 but also the transcription of gas2+ through Mbx2.

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Publicly available datasets were analyzed in this study. The original contributions presented in the study are included in the article/Supplementary Material.

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Acknowledgements

Part of this research was supported by the National Research Foundation of Korea (NRF) (Grant No. 2020R1F1A107307511 & 2021R1A2C10092291231482092640102).

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Author notes

  1. Won-Hwa Kang

    Present address: Y-Biologics Co. Ltd., Daejeon, 34013, Republic of Korea

  2. Yoon-Dong Park

    Present address: Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD, 20814, USA

  3. Joo-Yeon Lim

    Present address: Department of Microbiology and Immunology, Indiana University School of Medicine-Terre Haute, Terre Haute, IN, 47809, USA

  4. Won-Hwa Kang and Yoon-Dong Park contributed equally to this work.

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  1. Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon, 34134, Republic of Korea

    Won-Hwa Kang, Yoon-Dong Park, Joo-Yeon Lim & Hee-Moon Park

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Kang, WH., Park, YD., Lim, JY. et al. LAMMER Kinase Governs the Expression and Cellular Localization of Gas2, a Key Regulator of Flocculation in Schizosaccharomyces pombe. J Microbiol. 62, 21–31 (2024). https://doi.org/10.1007/s12275-023-00097-7

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