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Whole-genome sequencing analysis of Shiga toxin-producing Escherichia coli O22:H8 isolated from cattle prediction pathogenesis and colonization factors and position in STEC universe phylogeny

  • Microbial Genetics, Genomics and Molecular Biology
  • Published:
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Abstract

Shiga toxin-producing Escherichia coli (STEC) is a foodborne pathogen capable of causing illness in humans. In a previous study, our group showed that a STEC isolate belonging to O22:H8 serotype (strain 154) can interfere with STEC O157:H7 colonization both in vitro and in vivo. Using whole-genome sequencing and genomic comparative, we predicted a subset of genes acquired by O22:H8 strain 154 through horizontal gene transfer that might be responsible for the phenotype previously described by our group. Among them were identified genes related to the pathogenesis of non-LEE (locus of enterocyte effacement) STEC, specific metabolic processes, antibiotic resistance and genes encoding for the T6SS-1 that is related to inter-bacterial competition. In addition, we showed that this strain carries stx1c and stx2dact, a mucus-inducible variant. The results obtained in this study provide insights into STEC genomic plasticity and the importance of genomic islands in the adaptation and pathogenesis of this pathogen.

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Data Availability

The Whole-Genome Shotgun project has been deposited in the GenBank database under the accession number CP-067426-CP067431.

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Acknowledgements

We thank the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and Ministerio de Ciencia, Tecnología e Innovación (MINCyT).

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Authors and Affiliations

  1. Agrobiotechnology and Molecular Biology Institute (IABIMO-INTA/CONICET), Hurlingham, B1686IGC, Argentina

    Wanderson Marques Da Silva, Mariano Larzabal, Nahuel Riviere & Angel Cataldi

  2. Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte, 31270-901, Brazil

    Flavia Figueira Aburjaile

  3. Institute of Veterinary Pathobiology (IPEVET-INTA/CONICET), Hurlingham, B1686WAA, Argentina

    Luisina Martorelli

  4. Agricultural Research Service, U.S. Department of Agriculture, U.S. Meat Animal Research Center, Clay Center, Nebraska, 68933, USA

    James Bono

  5. Dairy Chain Research Institute (IDICAL-INTA/CONICET), Rafaela 2300, Santa Fe, S2300, Argentina

    Ariel Amadio

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  1. Wanderson Marques Da Silva
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  2. Mariano Larzabal
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Da Silva, W.M., Larzabal, M., Aburjaile, F.F. et al. Whole-genome sequencing analysis of Shiga toxin-producing Escherichia coli O22:H8 isolated from cattle prediction pathogenesis and colonization factors and position in STEC universe phylogeny. J Microbiol. 60, 689–704 (2022). https://doi.org/10.1007/s12275-022-1616-z

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