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Vagococcus zengguangii sp. nov., isolated from yak faeces

  • Microbial Systematics and Evolutionary Microbiology
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Abstract

Two unknown Gram-stain-positive, catalase- and oxidasenegative, non-motile, and coccus-shaped bacteria, designated MN-17T and MN-09, were isolated from yaks faeces (Bos grunniens) in the Qinghai-Tibet Plateau of China. 16S rRNA gene sequence-based comparative analyses revealed that the two strains were grouped within the genus Vagococcus, displaying the highest similarity with Vagococcus xieshaowenii CGMCC 1.16436T (98.6%) and Vagococcus elongatus CCUG 51432T (96.4%). Both strains grew optimally at 37°C and pH 7.0 in the presence of 0.5% (w/v) NaCl. The complete genome of MN-17T comprises 2,085 putative genes with a total of 2,190,262 bp and an average G + C content of 36.7 mol%. The major fatty acids were C16:0 (31.2%), C14:0 (28.5%), and C18:1ω9c (13.0%); the predominant respiratory quinone was MK-7 (68.8%); the peptidoglycan type was A4α(l-Lys-d-Asp); and the major polar lipid was diphosphatidylglycerol. Together, these supported the affiliation of strain MN-17T to the genus Vagococcus. In silico DNA-DNA hybridization and the average nucleotide identity values between MN-17T and all recognized species in the genus were 21.6–26.1% and 70.7–83.0%, respectively. MN-17T produced acid from d-cellobiose, d-fructose, glycerol, d-glucose, N-acetyl-glucosamine, gentiobiose, d-mannose, d-maltose, d-ribose, d-saccharose, salicin, d-trehalose, and d-xylose. These results distinguished MN-17T and MN-09 from closely related species in Vagococcus. Thus, we propose that strains MN-17T and MN-09 represent a novel species in the genus Vagococcus, with the name Vagococcus zengguangii sp. The type strain is MN-17T (= CGMCC 1.16726T = GDMCC 1.1589T = JCM 33478T).

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Acknowledgments

This work was supported by grants from National Science and Technology Major Project of China (2018ZX10712001-018, 2017ZX10303405-002, 2017ZX10303405-002-005), National Science and Technology of China (2017FY101202), National Key R&D Program of China (2019YFC1200500 and 2019YFC1200505), Sanming Project of Medicine in Shenzhen (SZSM201811071) and Research Units of Discovery of Unknown Bacteria and Function (2018RU010).

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Authors and Affiliations

  1. Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, P. R. China

    Yajun Ge, Dong Jin, Jing Yang, Shan Lu, Xiaoyan Zhang & Jianguo Xu

  2. Chinese Center for Disease Control and Prevention, State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Changping, Beijing, 102206, P. R. China

    Dong Jin, Jing Yang, Shan Lu, Ying Huang, Han Zheng & Jianguo Xu

  3. Research Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing, P. R. China

    Dong Jin, Jing Yang, Shan Lu & Jianguo Xu

  4. Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, Shangqiu Normal University, Shangqiu, 476000, P. R. China

    Xin-He Lai

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  1. Yajun Ge
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Correspondence to Xiaoyan Zhang or Jianguo Xu.

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12275_2021_406_MOESM1_ESM.pdf

Supplementary data Table S1. Characteristics that differentiate V. zengguangii sp. from other species in the genus Vagococcus

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Ge, Y., Jin, D., Lai, XH. et al. Vagococcus zengguangii sp. nov., isolated from yak faeces. J Microbiol. 59, 1–9 (2021). https://doi.org/10.1007/s12275-021-0406-3

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